Aspergillus pseudonomiae
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13384 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N6IHN6|A0A5N6IHN6_9EURO Uncharacterized protein OS=Aspergillus pseudonomiae OX=1506151 GN=BDV32DRAFT_3048 PE=4 SV=1
MM1 pKa = 7.39 YY2 pKa = 10.72 LNQLVLAGLALCGSALASPTPAKK25 pKa = 10.44 RR26 pKa = 11.84 ADD28 pKa = 3.67 EE29 pKa = 4.22 KK30 pKa = 11.29 VGYY33 pKa = 10.41 LSVYY37 pKa = 6.69 WTTDD41 pKa = 3.14 DD42 pKa = 3.55 EE43 pKa = 4.89 SVYY46 pKa = 10.52 FALSDD51 pKa = 3.6 NDD53 pKa = 4.0 DD54 pKa = 4.0 PLGFSTINSGSAVVSPTLGTKK75 pKa = 9.92 AVRR78 pKa = 11.84 DD79 pKa = 3.6 TSIIAGQGDD88 pKa = 3.86 DD89 pKa = 4.0 AGKK92 pKa = 10.19 YY93 pKa = 8.8 WIIGTDD99 pKa = 3.63 LNIGEE104 pKa = 4.77 TTWDD108 pKa = 3.25 AAVRR112 pKa = 11.84 TGSRR116 pKa = 11.84 AIYY119 pKa = 9.3 VWEE122 pKa = 4.19 STDD125 pKa = 3.71 LVNWSEE131 pKa = 4.12 NTLVTVEE138 pKa = 4.92 DD139 pKa = 3.99 STAGMVWAPDD149 pKa = 4.56 AIWDD153 pKa = 4.1 PEE155 pKa = 3.82 QGQYY159 pKa = 9.27 FVHH162 pKa = 6.3 WASRR166 pKa = 11.84 FYY168 pKa = 11.16 AADD171 pKa = 3.85 DD172 pKa = 4.0 TEE174 pKa = 4.5 HH175 pKa = 6.64 TGDD178 pKa = 3.38 ATTGNVLRR186 pKa = 11.84 YY187 pKa = 9.93 AYY189 pKa = 9.52 TSDD192 pKa = 3.08 FKK194 pKa = 10.96 TFSEE198 pKa = 4.19 PQDD201 pKa = 4.06 YY202 pKa = 10.67 IVGTTDD208 pKa = 4.8 VIDD211 pKa = 3.8 LCLIQLDD218 pKa = 4.55 SNTLLRR224 pKa = 11.84 SYY226 pKa = 10.95 VNSSSADD233 pKa = 3.47 GLPVEE238 pKa = 4.9 ISTNGLLGDD247 pKa = 3.87 WSLLGSIADD256 pKa = 3.71 SAGYY260 pKa = 7.29 EE261 pKa = 3.92 APYY264 pKa = 10.4 FFADD268 pKa = 3.27 NAGGGKK274 pKa = 10.58 GYY276 pKa = 7.74 MTADD280 pKa = 3.72 LVGSSPGISGWTSDD294 pKa = 4.14 DD295 pKa = 3.65 LSSGVLTKK303 pKa = 10.13 DD304 pKa = 3.01 TSHH307 pKa = 7.41 DD308 pKa = 3.67 LTFMRR313 pKa = 11.84 HH314 pKa = 4.69 NSVLAVTQSQYY325 pKa = 11.62 DD326 pKa = 3.51 ALKK329 pKa = 11.21 AMM331 pKa = 5.08
Molecular weight: 35.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.884
IPC_protein 3.923
Toseland 3.681
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.872
Rodwell 3.732
Grimsley 3.592
Solomon 3.91
Lehninger 3.872
Nozaki 4.024
DTASelect 4.317
Thurlkill 3.745
EMBOSS 3.884
Sillero 4.037
Patrickios 1.303
IPC_peptide 3.91
IPC2_peptide 4.012
IPC2.peptide.svr19 3.908
Protein with the highest isoelectric point:
>tr|A0A5N6HXM4|A0A5N6HXM4_9EURO Uncharacterized protein OS=Aspergillus pseudonomiae OX=1506151 GN=BDV32DRAFT_150549 PE=4 SV=1
MM1 pKa = 7.96 DD2 pKa = 6.01 SIGSTGRR9 pKa = 11.84 GRR11 pKa = 11.84 NKK13 pKa = 9.9 IQLLKK18 pKa = 9.35 PSRR21 pKa = 11.84 MRR23 pKa = 11.84 SRR25 pKa = 11.84 TLVHH29 pKa = 5.19 VTFSGVLLRR38 pKa = 11.84 LLTGLLLVYY47 pKa = 10.16 RR48 pKa = 11.84 HH49 pKa = 6.58 SILRR53 pKa = 11.84 AYY55 pKa = 9.97 VIGYY59 pKa = 9.58 LSSTTPRR66 pKa = 11.84 VIASLRR72 pKa = 11.84 QIWRR76 pKa = 11.84 NDD78 pKa = 2.78 LDD80 pKa = 3.91 YY81 pKa = 10.36 QQKK84 pKa = 10.04 RR85 pKa = 11.84 RR86 pKa = 11.84 VLVRR90 pKa = 11.84 ALTSAIRR97 pKa = 11.84 LDD99 pKa = 3.91 SFPTFCASLVGGSTVFPLVIFRR121 pKa = 11.84 LAEE124 pKa = 4.06 LLSQKK129 pKa = 10.29 VDD131 pKa = 4.51 FKK133 pKa = 11.2 CSRR136 pKa = 11.84 LEE138 pKa = 3.66 SARR141 pKa = 11.84 FLRR144 pKa = 11.84 LARR147 pKa = 11.84 LICTFVSAWLSFEE160 pKa = 4.12 LLNRR164 pKa = 11.84 KK165 pKa = 8.93 PIRR168 pKa = 11.84 LQGIEE173 pKa = 4.28 GTLADD178 pKa = 4.35 NGSDD182 pKa = 3.26 EE183 pKa = 5.23 AAGKK187 pKa = 8.45 QQGCQNTIHH196 pKa = 6.42 NRR198 pKa = 11.84 PQFAGRR204 pKa = 11.84 TMDD207 pKa = 3.33 LTLFSFTRR215 pKa = 11.84 AVDD218 pKa = 4.12 LVACIIWARR227 pKa = 11.84 WRR229 pKa = 11.84 RR230 pKa = 11.84 WRR232 pKa = 11.84 SARR235 pKa = 11.84 GRR237 pKa = 11.84 WSRR240 pKa = 11.84 AEE242 pKa = 3.86 SLAPKK247 pKa = 9.11 LTDD250 pKa = 2.9 SGVFAASAAIVMWAWFYY267 pKa = 11.4 LPEE270 pKa = 4.93 RR271 pKa = 11.84 LPKK274 pKa = 10.42 SYY276 pKa = 10.54 GKK278 pKa = 9.77 WIGEE282 pKa = 4.12 VAKK285 pKa = 10.63 VDD287 pKa = 3.53 SRR289 pKa = 11.84 LIEE292 pKa = 3.67 ALRR295 pKa = 11.84 RR296 pKa = 11.84 VRR298 pKa = 11.84 RR299 pKa = 11.84 GVFVYY304 pKa = 10.28 GKK306 pKa = 8.77 DD307 pKa = 3.14 TGQAPLLQSMCKK319 pKa = 10.25 DD320 pKa = 3.61 YY321 pKa = 10.93 GWPIEE326 pKa = 4.2 WGDD329 pKa = 3.57 PAKK332 pKa = 10.04 TIPIPCEE339 pKa = 3.88 MVHH342 pKa = 6.01 MSCGPNCEE350 pKa = 3.69 KK351 pKa = 10.63 HH352 pKa = 5.5 AVSRR356 pKa = 11.84 FVRR359 pKa = 11.84 TFGFACATYY368 pKa = 10.22 IPLQILFRR376 pKa = 11.84 LKK378 pKa = 10.31 RR379 pKa = 11.84 LKK381 pKa = 10.66 SVLSLRR387 pKa = 11.84 RR388 pKa = 11.84 AVSDD392 pKa = 3.56 ALRR395 pKa = 11.84 SSVFLASFVSIFYY408 pKa = 10.75 YY409 pKa = 9.68 SVCLARR415 pKa = 11.84 TRR417 pKa = 11.84 IGPKK421 pKa = 9.43 VFPRR425 pKa = 11.84 DD426 pKa = 3.24 VVTPMMWDD434 pKa = 2.97 SGLCVGAGCLMCGWSILVEE453 pKa = 4.44 SPSKK457 pKa = 10.38 RR458 pKa = 11.84 QEE460 pKa = 3.62 LALFVAPRR468 pKa = 11.84 AAATVLPRR476 pKa = 11.84 FYY478 pKa = 10.89 DD479 pKa = 2.98 KK480 pKa = 10.71 QYY482 pKa = 10.8 QYY484 pKa = 11.29 RR485 pKa = 11.84 EE486 pKa = 4.17 RR487 pKa = 11.84 ITFAVSAALLLTCLQEE503 pKa = 4.15 RR504 pKa = 11.84 PGLVRR509 pKa = 11.84 GVFGRR514 pKa = 11.84 IATSVLKK521 pKa = 10.85
Molecular weight: 58.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.663
IPC_protein 10.496
Toseland 10.599
ProMoST 10.35
Dawson 10.716
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 10.877
Grimsley 10.774
Solomon 10.818
Lehninger 10.789
Nozaki 10.613
DTASelect 10.438
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.657
Patrickios 10.54
IPC_peptide 10.818
IPC2_peptide 9.721
IPC2.peptide.svr19 8.496
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13384
0
13384
6142267
49
6889
458.9
50.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.247 ± 0.017
1.374 ± 0.009
5.563 ± 0.015
5.999 ± 0.021
3.86 ± 0.013
6.789 ± 0.015
2.469 ± 0.009
5.146 ± 0.017
4.539 ± 0.016
9.255 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.181 ± 0.008
3.678 ± 0.008
5.888 ± 0.022
4.023 ± 0.015
6.031 ± 0.018
8.251 ± 0.025
5.934 ± 0.013
6.273 ± 0.016
1.536 ± 0.008
2.962 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here