Staphylococcus phage phiNM3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Biseptimavirus; Staphylococcus virus phiNM3

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0EWY1|A0EWY1_9CAUD Uncharacterized protein OS=Staphylococcus phage phiNM3 OX=387909 PE=4 SV=1
MM1 pKa = 7.51NNRR4 pKa = 11.84EE5 pKa = 4.1QIEE8 pKa = 3.98QSVISASAYY17 pKa = 10.04NGNDD21 pKa = 3.29TEE23 pKa = 4.52GLLKK27 pKa = 10.49EE28 pKa = 4.44IEE30 pKa = 4.24DD31 pKa = 4.28VYY33 pKa = 11.57KK34 pKa = 10.64KK35 pKa = 10.72AQAFDD40 pKa = 4.61EE41 pKa = 4.37ILEE44 pKa = 4.31GMTNAIQHH52 pKa = 5.16SVKK55 pKa = 10.6EE56 pKa = 4.24GVEE59 pKa = 3.63LDD61 pKa = 3.64EE62 pKa = 5.37AVGIMAGQVVYY73 pKa = 10.26KK74 pKa = 10.69YY75 pKa = 10.72EE76 pKa = 3.9EE77 pKa = 4.01EE78 pKa = 4.27QEE80 pKa = 4.12NEE82 pKa = 4.0HH83 pKa = 6.86

Molecular weight: 9.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0EWX8|A0EWX8_9CAUD Uncharacterized protein OS=Staphylococcus phage phiNM3 OX=387909 PE=4 SV=1
MM1 pKa = 7.29ARR3 pKa = 11.84KK4 pKa = 9.59ARR6 pKa = 11.84IVTINDD12 pKa = 2.81KK13 pKa = 10.44PYY15 pKa = 10.06RR16 pKa = 11.84FSKK19 pKa = 11.02FEE21 pKa = 3.76MEE23 pKa = 5.0LIEE26 pKa = 4.34SHH28 pKa = 6.88GITAGMVSKK37 pKa = 10.43RR38 pKa = 11.84VKK40 pKa = 10.67DD41 pKa = 3.3GWEE44 pKa = 3.56LHH46 pKa = 5.97EE47 pKa = 6.15AMDD50 pKa = 4.24APEE53 pKa = 4.22GTRR56 pKa = 11.84LSEE59 pKa = 3.86YY60 pKa = 10.23RR61 pKa = 11.84EE62 pKa = 3.95KK63 pKa = 10.39KK64 pKa = 8.51TIEE67 pKa = 3.99RR68 pKa = 11.84LEE70 pKa = 3.92QARR73 pKa = 11.84LEE75 pKa = 4.26RR76 pKa = 11.84KK77 pKa = 9.75LEE79 pKa = 4.03RR80 pKa = 11.84KK81 pKa = 9.41RR82 pKa = 11.84KK83 pKa = 9.67RR84 pKa = 11.84EE85 pKa = 3.71AEE87 pKa = 3.97LRR89 pKa = 11.84RR90 pKa = 11.84KK91 pKa = 9.76KK92 pKa = 10.2PHH94 pKa = 6.56LFNVPQKK101 pKa = 9.93HH102 pKa = 4.99PRR104 pKa = 11.84RR105 pKa = 11.84RR106 pKa = 11.84YY107 pKa = 9.11CVLPVGKK114 pKa = 10.03RR115 pKa = 11.84HH116 pKa = 5.84IRR118 pKa = 11.84EE119 pKa = 4.08SS120 pKa = 3.36

Molecular weight: 14.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

12774

33

1509

196.5

22.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.605 ± 0.439

0.462 ± 0.083

6.302 ± 0.325

7.891 ± 0.512

4.047 ± 0.298

5.644 ± 0.708

1.808 ± 0.18

7.821 ± 0.342

9.739 ± 0.38

8.079 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.349 ± 0.197

6.889 ± 0.306

2.591 ± 0.163

3.992 ± 0.305

4.259 ± 0.276

5.793 ± 0.291

5.574 ± 0.252

5.84 ± 0.218

1.033 ± 0.184

4.282 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski