Yersinia phage phiR1-37

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 367 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4KKM5|G4KKM5_9CAUD Homing endonuclease family OS=Yersinia phage phiR1-37 OX=331278 GN=g287 PE=4 SV=1
MM1 pKa = 7.46IVPIIIHH8 pKa = 6.15PNEE11 pKa = 4.25TNDD14 pKa = 3.43SFGVSVPGLKK24 pKa = 10.46GCFSAGDD31 pKa = 3.8TLEE34 pKa = 4.55EE35 pKa = 3.94ATQNVAEE42 pKa = 4.49ALDD45 pKa = 4.55LYY47 pKa = 11.16VEE49 pKa = 4.6HH50 pKa = 6.77CHH52 pKa = 6.3EE53 pKa = 4.62EE54 pKa = 4.24EE55 pKa = 4.59INVNGNGKK63 pKa = 9.99VMINTNIEE71 pKa = 3.92HH72 pKa = 7.3AFDD75 pKa = 3.5QSEE78 pKa = 4.19GGIVVCIMYY87 pKa = 10.46EE88 pKa = 3.76NDD90 pKa = 3.53DD91 pKa = 4.03AVSVDD96 pKa = 3.12ISS98 pKa = 3.31

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4KKA1|G4KKA1_9CAUD G162 protein OS=Yersinia phage phiR1-37 OX=331278 GN=g162 PE=4 SV=1
MM1 pKa = 7.55VEE3 pKa = 4.09TNTYY7 pKa = 8.18TVHH10 pKa = 5.07STRR13 pKa = 11.84LKK15 pKa = 10.56RR16 pKa = 11.84IVNPILRR23 pKa = 11.84KK24 pKa = 8.83IQYY27 pKa = 7.7RR28 pKa = 11.84TNTPYY33 pKa = 10.93VIASEE38 pKa = 4.28FDD40 pKa = 3.16SSKK43 pKa = 9.79NTFIRR48 pKa = 11.84YY49 pKa = 8.61AIRR52 pKa = 11.84RR53 pKa = 11.84VGYY56 pKa = 9.93DD57 pKa = 3.15RR58 pKa = 11.84NVDD61 pKa = 3.75PDD63 pKa = 3.72KK64 pKa = 10.91STCIYY69 pKa = 10.51LKK71 pKa = 9.13TGKK74 pKa = 9.94EE75 pKa = 3.38IRR77 pKa = 11.84ITIKK81 pKa = 10.56YY82 pKa = 8.98YY83 pKa = 9.72IRR85 pKa = 11.84KK86 pKa = 8.93LFKK89 pKa = 10.62

Molecular weight: 10.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

367

0

367

81518

21

2553

222.1

25.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.173 ± 0.194

0.889 ± 0.055

6.437 ± 0.116

6.606 ± 0.182

5.39 ± 0.097

4.956 ± 0.264

1.651 ± 0.081

8.089 ± 0.14

8.471 ± 0.192

8.469 ± 0.133

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.622 ± 0.074

6.944 ± 0.113

3.085 ± 0.063

2.468 ± 0.074

3.965 ± 0.093

7.699 ± 0.146

5.921 ± 0.152

5.969 ± 0.096

0.655 ± 0.038

4.54 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski