Freshwater phage uvFW-CGR-AMD-COM-C449

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0XU86|A0A1B0XU86_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMD-COM-C449 OX=1838155 GN=uvFWCGRAMDCOMC449_041 PE=4 SV=1
MM1 pKa = 7.2SAQYY5 pKa = 9.55FAKK8 pKa = 10.52LDD10 pKa = 3.81GNNVVVDD17 pKa = 3.93VAVTTAEE24 pKa = 4.04FMAANPDD31 pKa = 4.01RR32 pKa = 11.84YY33 pKa = 9.84PGIWVEE39 pKa = 3.86TFINVEE45 pKa = 3.94GKK47 pKa = 8.13TYY49 pKa = 10.98AGVGYY54 pKa = 8.07TFDD57 pKa = 3.52GTDD60 pKa = 3.84FVAPIVEE67 pKa = 4.3EE68 pKa = 4.47SVV70 pKa = 3.09

Molecular weight: 7.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0XU83|A0A1B0XU83_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMD-COM-C449 OX=1838155 GN=uvFWCGRAMDCOMC449_019 PE=4 SV=1
MM1 pKa = 6.48TTLEE5 pKa = 3.94AVGIFTSAVCLAFPLWGAYY24 pKa = 8.61ILRR27 pKa = 11.84AYY29 pKa = 10.49LDD31 pKa = 3.78NKK33 pKa = 7.8PTRR36 pKa = 11.84AEE38 pKa = 3.65RR39 pKa = 11.84WHH41 pKa = 6.75RR42 pKa = 11.84NHH44 pKa = 6.66EE45 pKa = 4.13NVLRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84EE52 pKa = 4.01QINRR56 pKa = 11.84RR57 pKa = 11.84GWW59 pKa = 3.09

Molecular weight: 7.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

10553

30

1851

178.9

19.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.357 ± 0.43

0.768 ± 0.208

5.496 ± 0.303

5.524 ± 0.347

2.938 ± 0.184

8.746 ± 0.814

1.374 ± 0.21

5.24 ± 0.245

5.941 ± 0.508

7.514 ± 0.341

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.625 ± 0.203

4.406 ± 0.184

4.53 ± 0.334

4.16 ± 0.339

5.098 ± 0.351

5.96 ± 0.371

7.912 ± 0.67

6.519 ± 0.345

1.753 ± 0.176

3.137 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski