Morchella conica CCBAS932
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11578 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N4KT50|A0A3N4KT50_9PEZI Endoplasmic reticulum transmembrane protein OS=Morchella conica CCBAS932 OX=1392247 GN=P167DRAFT_534724 PE=3 SV=1
MM1 pKa = 7.49 GNAPIQVVRR10 pKa = 11.84 CSALCVHH17 pKa = 6.25 EE18 pKa = 4.39 TFNDD22 pKa = 3.35 EE23 pKa = 6.27 DD24 pKa = 3.98 EE25 pKa = 6.28 DD26 pKa = 4.18 EE27 pKa = 6.24 DD28 pKa = 4.16 EE29 pKa = 6.65 DD30 pKa = 4.31 EE31 pKa = 6.65 DD32 pKa = 4.31 EE33 pKa = 6.65 DD34 pKa = 4.31 EE35 pKa = 6.65 DD36 pKa = 4.31 EE37 pKa = 6.65 DD38 pKa = 4.31 EE39 pKa = 6.65 DD40 pKa = 4.31 EE41 pKa = 6.65 DD42 pKa = 4.31 EE43 pKa = 6.69 DD44 pKa = 4.16 EE45 pKa = 6.49 DD46 pKa = 4.16 EE47 pKa = 6.09 DD48 pKa = 3.86 EE49 pKa = 5.59 GEE51 pKa = 4.46 GEE53 pKa = 4.91 GEE55 pKa = 4.52 GEE57 pKa = 4.03 GDD59 pKa = 3.39 KK60 pKa = 11.49 AGGDD64 pKa = 3.78 DD65 pKa = 3.64 YY66 pKa = 11.87 KK67 pKa = 11.39 KK68 pKa = 10.56 NCEE71 pKa = 3.63 FLLRR75 pKa = 11.84 FVSVGPGALPHH86 pKa = 6.37 PFSYY90 pKa = 10.88 VSSKK94 pKa = 11.16 SLDD97 pKa = 3.62 PACTNLSTCLGDD109 pKa = 3.46 MQHH112 pKa = 6.08 NAEE115 pKa = 4.3 SEE117 pKa = 4.34 VTRR120 pKa = 11.84 ICQTSSLVCWNQGPVV135 pKa = 2.85
Molecular weight: 14.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.643
IPC_protein 3.63
Toseland 3.427
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.528
Rodwell 3.465
Grimsley 3.338
Solomon 3.605
Lehninger 3.554
Nozaki 3.732
DTASelect 3.91
Thurlkill 3.478
EMBOSS 3.541
Sillero 3.757
Patrickios 1.024
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.696
Protein with the highest isoelectric point:
>tr|A0A3N4LHE4|A0A3N4LHE4_9PEZI Ankyrin OS=Morchella conica CCBAS932 OX=1392247 GN=P167DRAFT_127481 PE=4 SV=1
MM1 pKa = 7.85 PLTTRR6 pKa = 11.84 RR7 pKa = 11.84 AHH9 pKa = 5.92 ATRR12 pKa = 11.84 STTVARR18 pKa = 11.84 KK19 pKa = 8.47 PSLMYY24 pKa = 10.81 RR25 pKa = 11.84 LTHH28 pKa = 6.04 PAGTRR33 pKa = 11.84 TVGTRR38 pKa = 11.84 STAGTRR44 pKa = 11.84 ATRR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 TKK51 pKa = 9.79 ATGPAPVSTHH61 pKa = 4.44 RR62 pKa = 11.84 RR63 pKa = 11.84 ARR65 pKa = 11.84 TKK67 pKa = 8.65 PTLGARR73 pKa = 11.84 IHH75 pKa = 6.54 GMAKK79 pKa = 9.81 KK80 pKa = 10.38 VAGTLTGSRR89 pKa = 11.84 TKK91 pKa = 10.35 KK92 pKa = 9.47 AQGNAMVNGRR102 pKa = 11.84 TPGSRR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 RR110 pKa = 11.84 HH111 pKa = 5.13 LL112 pKa = 3.58
Molecular weight: 12.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.374
IPC_protein 12.822
Toseland 12.983
ProMoST 13.481
Dawson 12.983
Bjellqvist 12.983
Wikipedia 13.466
Rodwell 12.618
Grimsley 13.027
Solomon 13.481
Lehninger 13.378
Nozaki 12.983
DTASelect 12.983
Thurlkill 12.983
EMBOSS 13.481
Sillero 12.983
Patrickios 12.325
IPC_peptide 13.481
IPC2_peptide 12.471
IPC2.peptide.svr19 9.199
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11577
1
11578
4742244
49
5498
409.6
45.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.19 ± 0.024
1.262 ± 0.01
5.329 ± 0.017
6.692 ± 0.029
3.609 ± 0.015
7.155 ± 0.024
2.357 ± 0.009
5.005 ± 0.017
5.222 ± 0.024
8.71 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.217 ± 0.009
3.706 ± 0.015
6.129 ± 0.028
3.636 ± 0.024
6.138 ± 0.02
8.088 ± 0.027
6.184 ± 0.022
6.238 ± 0.017
1.363 ± 0.009
2.766 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here