Streptococcus satellite phage Javan391

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLA6|A0A4D5ZLA6_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan391 OX=2558674 GN=JavanS391_0021 PE=3 SV=1
MM1 pKa = 7.2KK2 pKa = 9.64TLSDD6 pKa = 3.59TPKK9 pKa = 9.76TFTFTTTLPNFEE21 pKa = 4.31QAQKK25 pKa = 10.83AGSALIGYY33 pKa = 7.54MIGTYY38 pKa = 7.49EE39 pKa = 4.14QSAIDD44 pKa = 3.19ITYY47 pKa = 9.12TGNGNLKK54 pKa = 10.61AEE56 pKa = 4.23YY57 pKa = 9.71TSDD60 pKa = 3.47KK61 pKa = 11.18DD62 pKa = 3.45LTEE65 pKa = 3.99SFEE68 pKa = 5.38RR69 pKa = 11.84ITNSLNHH76 pKa = 6.52TDD78 pKa = 4.41DD79 pKa = 4.37FEE81 pKa = 6.99DD82 pKa = 4.69YY83 pKa = 11.06EE84 pKa = 6.23DD85 pKa = 5.59DD86 pKa = 6.03FEE88 pKa = 8.21DD89 pKa = 5.79DD90 pKa = 4.55DD91 pKa = 4.4LTIKK95 pKa = 10.91GDD97 pKa = 3.69LDD99 pKa = 3.73TYY101 pKa = 9.69TALIGSFNTLGEE113 pKa = 4.17AQAFTEE119 pKa = 4.58NLDD122 pKa = 4.44DD123 pKa = 6.72DD124 pKa = 4.55LTNGNNFIFEE134 pKa = 4.26QLPDD138 pKa = 3.73TVVLYY143 pKa = 8.74VSPQDD148 pKa = 3.9TIASSTIQKK157 pKa = 9.78LQEE160 pKa = 4.07AYY162 pKa = 10.05VKK164 pKa = 10.73HH165 pKa = 5.21EE166 pKa = 4.43AEE168 pKa = 4.28YY169 pKa = 10.95EE170 pKa = 3.85PDD172 pKa = 3.49TFRR175 pKa = 11.84LSYY178 pKa = 10.89HH179 pKa = 6.44DD180 pKa = 3.81MRR182 pKa = 11.84VSQLQEE188 pKa = 3.77LDD190 pKa = 3.5KK191 pKa = 11.05DD192 pKa = 3.88TLINDD197 pKa = 4.1IIAYY201 pKa = 9.15EE202 pKa = 4.44LEE204 pKa = 4.08LLDD207 pKa = 3.89YY208 pKa = 11.08ADD210 pKa = 5.15RR211 pKa = 11.84LLSDD215 pKa = 3.93EE216 pKa = 5.06PLPLSDD222 pKa = 3.05QHH224 pKa = 7.67GFEE227 pKa = 4.53TIEE230 pKa = 4.04LLGDD234 pKa = 3.73DD235 pKa = 4.96VIDD238 pKa = 4.37LVKK241 pKa = 10.76EE242 pKa = 3.72LDD244 pKa = 3.68TNKK247 pKa = 10.01EE248 pKa = 3.34FDD250 pKa = 4.86GIHH253 pKa = 6.46SYY255 pKa = 10.62IIGGG259 pKa = 3.63

Molecular weight: 29.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZLK0|A0A4D5ZLK0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan391 OX=2558674 GN=JavanS391_0010 PE=4 SV=1
MM1 pKa = 7.85LITIEE6 pKa = 4.1HH7 pKa = 6.27AEE9 pKa = 4.08KK10 pKa = 10.85VRR12 pKa = 11.84VKK14 pKa = 10.5RR15 pKa = 11.84GRR17 pKa = 11.84LDD19 pKa = 3.36LTKK22 pKa = 10.58SEE24 pKa = 4.17TAKK27 pKa = 10.66RR28 pKa = 11.84MNVTPRR34 pKa = 11.84TLQKK38 pKa = 10.09IEE40 pKa = 3.99QGDD43 pKa = 3.45YY44 pKa = 10.91DD45 pKa = 4.06APKK48 pKa = 10.61KK49 pKa = 10.74SLLVLL54 pKa = 4.08

Molecular weight: 6.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

2874

40

525

136.9

15.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.628 ± 0.289

0.452 ± 0.12

6.263 ± 0.758

7.864 ± 0.724

4.071 ± 0.37

4.349 ± 0.365

1.427 ± 0.26

7.794 ± 0.374

10.16 ± 0.755

10.369 ± 0.633

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.644 ± 0.286

7.029 ± 0.425

3.166 ± 0.598

3.41 ± 0.357

4.106 ± 0.426

5.567 ± 0.422

6.089 ± 0.689

4.628 ± 0.402

0.905 ± 0.198

5.08 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski