Salmonella phage SE-W109

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; unclassified Jerseyvirus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A678P1K3|A0A678P1K3_9CAUD Uncharacterized protein OS=Salmonella phage SE-W109 OX=1897529 GN=SaThSEW109_0012 PE=4 SV=1
MM1 pKa = 8.57VEE3 pKa = 3.9EE4 pKa = 5.34HH5 pKa = 7.17YY6 pKa = 9.79DD7 pKa = 3.14TAYY10 pKa = 8.95ITDD13 pKa = 4.03TSPLLRR19 pKa = 11.84AFLYY23 pKa = 10.14TGLHH27 pKa = 6.14SPVQSLIDD35 pKa = 3.52AQGASDD41 pKa = 3.98ALL43 pKa = 3.89

Molecular weight: 4.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A678P6U2|A0A678P6U2_9CAUD Uncharacterized protein OS=Salmonella phage SE-W109 OX=1897529 GN=SaThSEW109_0024 PE=4 SV=1
MM1 pKa = 7.52SLATDD6 pKa = 3.7ILKK9 pKa = 10.24HH10 pKa = 6.28AGINLAPPSSAVTKK24 pKa = 10.32KK25 pKa = 7.19VTRR28 pKa = 11.84VKK30 pKa = 10.59EE31 pKa = 3.94KK32 pKa = 10.79VKK34 pKa = 10.47RR35 pKa = 11.84KK36 pKa = 8.52PKK38 pKa = 10.04PRR40 pKa = 11.84VKK42 pKa = 10.0PVNEE46 pKa = 4.14MPDD49 pKa = 3.55VYY51 pKa = 10.73PRR53 pKa = 11.84IPGVHH58 pKa = 4.6QPKK61 pKa = 9.16YY62 pKa = 10.53CVGKK66 pKa = 10.16GLWRR70 pKa = 11.84AHH72 pKa = 6.43SYY74 pKa = 10.54DD75 pKa = 3.01GKK77 pKa = 10.98KK78 pKa = 10.05VVNLGEE84 pKa = 4.35FSSQARR90 pKa = 11.84AHH92 pKa = 6.01MAVKK96 pKa = 10.46LYY98 pKa = 10.67KK99 pKa = 9.52LWRR102 pKa = 11.84KK103 pKa = 9.48RR104 pKa = 11.84GHH106 pKa = 6.17SNIPHH111 pKa = 6.42KK112 pKa = 10.53PSIRR116 pKa = 11.84LYY118 pKa = 8.83TFRR121 pKa = 6.0

Molecular weight: 13.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12593

39

903

199.9

22.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.585 ± 0.55

0.961 ± 0.15

6.019 ± 0.237

6.726 ± 0.441

3.835 ± 0.18

7.838 ± 0.353

1.715 ± 0.176

4.868 ± 0.197

6.027 ± 0.365

7.742 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.708 ± 0.148

4.304 ± 0.219

3.907 ± 0.236

3.732 ± 0.241

5.59 ± 0.218

5.964 ± 0.275

6.472 ± 0.297

7.194 ± 0.28

1.445 ± 0.153

3.367 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski