Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lactobacillus; Lactobacillus johnsonii

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1809 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q74JS7|Q74JS7_LACJO Glycos_transf_1 domain-containing protein OS=Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) OX=257314 GN=LJ_1028 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 10.25DD3 pKa = 3.41SEE5 pKa = 4.67IEE7 pKa = 4.34LIGQNAMNWLKK18 pKa = 11.07EE19 pKa = 3.96NIHH22 pKa = 6.41FIKK25 pKa = 10.41AGSDD29 pKa = 3.36IEE31 pKa = 4.52VATPLIGAYY40 pKa = 10.18GDD42 pKa = 5.79LVYY45 pKa = 10.82CWIEE49 pKa = 4.41KK50 pKa = 10.18EE51 pKa = 3.63DD52 pKa = 3.81DD53 pKa = 3.68YY54 pKa = 12.12YY55 pKa = 11.37RR56 pKa = 11.84ITDD59 pKa = 3.94DD60 pKa = 4.29GGTLFKK66 pKa = 10.79LDD68 pKa = 4.1PGQEE72 pKa = 4.01NMDD75 pKa = 4.27LLEE78 pKa = 4.13EE79 pKa = 4.43AMDD82 pKa = 3.67IVIGAGFEE90 pKa = 4.2FDD92 pKa = 4.3EE93 pKa = 4.72EE94 pKa = 4.3TLEE97 pKa = 4.17IYY99 pKa = 10.44QVVDD103 pKa = 3.13QDD105 pKa = 5.8DD106 pKa = 3.79IPATLNDD113 pKa = 3.67LTQLQVALTYY123 pKa = 10.46LASS126 pKa = 3.53

Molecular weight: 14.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q74L39|Q74L39_LACJO Uncharacterized protein OS=Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) OX=257314 GN=LJ_0386 PE=4 SV=1
MM1 pKa = 7.39MISIPIFILAIIIGIICLWVGFRR24 pKa = 11.84IKK26 pKa = 10.69GNQAVFGRR34 pKa = 11.84VLSTNRR40 pKa = 11.84QTWARR45 pKa = 11.84FWLTAGWVIIIIAGCYY61 pKa = 9.18LLFMIILHH69 pKa = 6.81FYY71 pKa = 11.31LKK73 pKa = 9.84MM74 pKa = 4.19

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1809

0

1809

587476

38

4734

324.8

36.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.026 ± 0.101

0.534 ± 0.016

5.901 ± 0.068

5.95 ± 0.067

4.406 ± 0.065

6.304 ± 0.057

1.921 ± 0.03

7.715 ± 0.063

7.978 ± 0.068

9.516 ± 0.098

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.541 ± 0.042

5.809 ± 0.076

3.531 ± 0.052

3.849 ± 0.037

3.541 ± 0.053

6.231 ± 0.14

5.705 ± 0.094

6.716 ± 0.053

1.066 ± 0.025

3.762 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski