Influenza A virus (strain A/Gull/Minnesota/945/1980 H13N6)
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q20NW0|M2_I80AD Matrix protein 2 OS=Influenza A virus (strain A/Gull/Minnesota/945/1980 H13N6) OX=385597 GN=M PE=3 SV=1
MM1 pKa = 7.38 SLLTEE6 pKa = 4.52 VEE8 pKa = 4.08 THH10 pKa = 5.77 TRR12 pKa = 11.84 SGWEE16 pKa = 4.0 CRR18 pKa = 11.84 CNDD21 pKa = 3.26 SSDD24 pKa = 3.85 PLVIAASIIGILHH37 pKa = 7.1 LILWILDD44 pKa = 3.39 RR45 pKa = 11.84 LFFKK49 pKa = 10.78 CIYY52 pKa = 9.95 RR53 pKa = 11.84 RR54 pKa = 11.84 LKK56 pKa = 10.55 YY57 pKa = 10.03 GLKK60 pKa = 10.09 RR61 pKa = 11.84 GPSTEE66 pKa = 4.02 GVPEE70 pKa = 4.23 SMRR73 pKa = 11.84 EE74 pKa = 3.7 EE75 pKa = 4.18 YY76 pKa = 10.29 QQEE79 pKa = 4.08 KK80 pKa = 10.22 QSAVDD85 pKa = 3.17 VDD87 pKa = 4.49 DD88 pKa = 3.92 GHH90 pKa = 6.42 FVNIEE95 pKa = 3.83 LEE97 pKa = 4.08
Molecular weight: 11.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.013
IPC2_protein 5.118
IPC_protein 4.978
Toseland 4.927
ProMoST 4.965
Dawson 4.965
Bjellqvist 5.143
Wikipedia 4.825
Rodwell 4.889
Grimsley 4.851
Solomon 4.965
Lehninger 4.927
Nozaki 5.092
DTASelect 5.207
Thurlkill 4.94
EMBOSS 4.876
Sillero 5.169
Patrickios 3.935
IPC_peptide 4.978
IPC2_peptide 5.169
IPC2.peptide.svr19 5.121
Protein with the highest isoelectric point:
>sp|Q0A3Y1|HEMA_I80AD Hemagglutinin OS=Influenza A virus (strain A/Gull/Minnesota/945/1980 H13N6) OX=385597 GN=HA PE=3 SV=1
MM1 pKa = 7.78 ASQGTKK7 pKa = 9.71 RR8 pKa = 11.84 SYY10 pKa = 10.66 EE11 pKa = 3.79 QMEE14 pKa = 4.56 TGGEE18 pKa = 3.87 RR19 pKa = 11.84 QNANDD24 pKa = 2.76 IRR26 pKa = 11.84 ASVGRR31 pKa = 11.84 MVGGIGRR38 pKa = 11.84 FYY40 pKa = 10.72 IQMCTEE46 pKa = 4.89 LKK48 pKa = 10.86 LSDD51 pKa = 3.5 NEE53 pKa = 3.85 GRR55 pKa = 11.84 LIQNSITIEE64 pKa = 4.06 RR65 pKa = 11.84 MVLSAFDD72 pKa = 3.57 EE73 pKa = 4.31 RR74 pKa = 11.84 RR75 pKa = 11.84 NKK77 pKa = 10.12 YY78 pKa = 10.38 LEE80 pKa = 4.07 EE81 pKa = 4.55 HH82 pKa = 7.1 PSTGRR87 pKa = 11.84 DD88 pKa = 3.37 PKK90 pKa = 10.32 KK91 pKa = 9.29 TGGPIYY97 pKa = 10.21 RR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 DD101 pKa = 3.62 GKK103 pKa = 8.78 WVRR106 pKa = 11.84 EE107 pKa = 3.66 LVLYY111 pKa = 10.56 DD112 pKa = 3.54 KK113 pKa = 11.5 EE114 pKa = 3.98 EE115 pKa = 3.79 LRR117 pKa = 11.84 RR118 pKa = 11.84 IWRR121 pKa = 11.84 QANNGEE127 pKa = 4.33 DD128 pKa = 3.23 ATAGLTHH135 pKa = 7.15 LMIWHH140 pKa = 6.74 SNLNDD145 pKa = 3.0 ATYY148 pKa = 10.71 QRR150 pKa = 11.84 TRR152 pKa = 11.84 ALVRR156 pKa = 11.84 TGMDD160 pKa = 2.98 PRR162 pKa = 11.84 MCSLMQGSTLPRR174 pKa = 11.84 RR175 pKa = 11.84 SGAAGAAVKK184 pKa = 10.56 GVGTMVMEE192 pKa = 5.61 LIRR195 pKa = 11.84 MIKK198 pKa = 10.22 RR199 pKa = 11.84 GVNDD203 pKa = 3.48 RR204 pKa = 11.84 NFWRR208 pKa = 11.84 GEE210 pKa = 3.54 NGRR213 pKa = 11.84 RR214 pKa = 11.84 TRR216 pKa = 11.84 IAYY219 pKa = 9.45 EE220 pKa = 3.95 RR221 pKa = 11.84 MCNILKK227 pKa = 10.75 GKK229 pKa = 9.34 FQTAAQRR236 pKa = 11.84 AMMDD240 pKa = 3.11 QVRR243 pKa = 11.84 EE244 pKa = 4.04 SRR246 pKa = 11.84 NPGNAEE252 pKa = 3.62 IEE254 pKa = 4.16 DD255 pKa = 4.8 LIFLARR261 pKa = 11.84 SALILRR267 pKa = 11.84 GAVAHH272 pKa = 6.58 KK273 pKa = 10.4 SCLPACVYY281 pKa = 10.62 GLAVASGYY289 pKa = 10.7 DD290 pKa = 3.72 FEE292 pKa = 6.06 RR293 pKa = 11.84 EE294 pKa = 4.56 GYY296 pKa = 9.53 SLVGIDD302 pKa = 5.28 PFRR305 pKa = 11.84 LLQNSQVFSLIRR317 pKa = 11.84 PNEE320 pKa = 4.06 NPAHH324 pKa = 6.86 KK325 pKa = 10.49 SQLVWMACHH334 pKa = 6.25 SAAFEE339 pKa = 4.24 DD340 pKa = 4.17 LRR342 pKa = 11.84 VSSFIRR348 pKa = 11.84 GTRR351 pKa = 11.84 VLPRR355 pKa = 11.84 GQLSTRR361 pKa = 11.84 GVQIASNEE369 pKa = 3.87 NMEE372 pKa = 4.49 TMNSSTLEE380 pKa = 3.86 LRR382 pKa = 11.84 SKK384 pKa = 10.2 YY385 pKa = 8.65 WAIRR389 pKa = 11.84 TRR391 pKa = 11.84 SGGNTNQQRR400 pKa = 11.84 ASAGQVSVQPTFSVQRR416 pKa = 11.84 NLPFEE421 pKa = 4.38 RR422 pKa = 11.84 ATIMAAFTGNPEE434 pKa = 3.67 GRR436 pKa = 11.84 TSDD439 pKa = 3.15 MRR441 pKa = 11.84 TEE443 pKa = 4.9 IIRR446 pKa = 11.84 MMEE449 pKa = 3.63 NSRR452 pKa = 11.84 PEE454 pKa = 4.02 DD455 pKa = 3.21 VSFQGRR461 pKa = 11.84 GVFEE465 pKa = 4.95 LSDD468 pKa = 3.67 EE469 pKa = 4.35 KK470 pKa = 10.77 ATNPIVPSFDD480 pKa = 3.22 MSNEE484 pKa = 3.24 GSYY487 pKa = 11.04 FFGDD491 pKa = 3.16 NAEE494 pKa = 4.48 EE495 pKa = 4.16 YY496 pKa = 11.1 DD497 pKa = 3.62 NN498 pKa = 4.9
Molecular weight: 56.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.936
IPC2_protein 8.858
IPC_protein 8.946
Toseland 9.385
ProMoST 9.297
Dawson 9.721
Bjellqvist 9.531
Wikipedia 9.94
Rodwell 9.823
Grimsley 9.809
Solomon 9.765
Lehninger 9.721
Nozaki 9.589
DTASelect 9.472
Thurlkill 9.56
EMBOSS 9.853
Sillero 9.692
Patrickios 5.143
IPC_peptide 9.765
IPC2_peptide 8.434
IPC2.peptide.svr19 7.776
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
4776
57
759
398.0
45.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.863 ± 0.37
1.843 ± 0.355
4.606 ± 0.189
7.601 ± 0.578
3.769 ± 0.313
6.533 ± 0.419
1.759 ± 0.163
6.742 ± 0.389
5.653 ± 0.443
8.019 ± 0.389
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.811 ± 0.417
5.59 ± 0.512
3.874 ± 0.248
3.874 ± 0.417
6.951 ± 0.604
7.286 ± 0.237
6.658 ± 0.338
5.36 ± 0.371
1.549 ± 0.157
2.659 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here