Yaba-like disease virus (YLDV)
Average proteome isoelectric point is 7.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 150 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9DHI9|Q9DHI9_YLDV 124R protein OS=Yaba-like disease virus OX=132475 GN=124R PE=4 SV=1
MM1 pKa = 7.95 IIPYY5 pKa = 9.96 IIVSCSLIILIVYY18 pKa = 8.93 LFLINKK24 pKa = 8.61 KK25 pKa = 8.59 YY26 pKa = 10.41 CKK28 pKa = 10.18 KK29 pKa = 10.33 FLKK32 pKa = 10.41 KK33 pKa = 10.01 KK34 pKa = 9.88 NKK36 pKa = 8.72 PVCIEE41 pKa = 4.01 LKK43 pKa = 10.69 KK44 pKa = 9.69 ITNTNKK50 pKa = 9.68 TSTLNSEE57 pKa = 4.61 DD58 pKa = 3.41 WTDD61 pKa = 3.29 MGSCEE66 pKa = 3.51 IYY68 pKa = 11.19 ANFRR72 pKa = 11.84 SSKK75 pKa = 9.78 RR76 pKa = 11.84 EE77 pKa = 3.53 KK78 pKa = 10.63 SFKK81 pKa = 10.98 LKK83 pKa = 10.71 DD84 pKa = 3.49 EE85 pKa = 4.29 QQNSSSTSSSDD96 pKa = 3.5 SEE98 pKa = 4.08 FDD100 pKa = 3.42 STINTDD106 pKa = 4.41 DD107 pKa = 4.17 EE108 pKa = 4.77 SEE110 pKa = 4.09 KK111 pKa = 11.14 LNWDD115 pKa = 3.52 NEE117 pKa = 4.37 EE118 pKa = 3.79 YY119 pKa = 10.45 KK120 pKa = 10.6 YY121 pKa = 11.02 DD122 pKa = 3.79 YY123 pKa = 10.66 GKK125 pKa = 10.36 SEE127 pKa = 4.05 STVYY131 pKa = 10.72 DD132 pKa = 4.25 LPQDD136 pKa = 4.25 NITEE140 pKa = 4.13 DD141 pKa = 3.16 TVYY144 pKa = 11.11 DD145 pKa = 3.57 VTDD148 pKa = 3.31 NVEE151 pKa = 4.32 NITYY155 pKa = 9.75 DD156 pKa = 3.77 LPQDD160 pKa = 3.98 SIIYY164 pKa = 10.31 DD165 pKa = 3.98 LDD167 pKa = 3.83 GKK169 pKa = 11.15 SDD171 pKa = 3.48 VAVYY175 pKa = 9.31 DD176 pKa = 3.63 IPEE179 pKa = 4.39 SEE181 pKa = 4.63 DD182 pKa = 3.32 NVYY185 pKa = 10.99 EE186 pKa = 4.13 NNICLEE192 pKa = 4.06 SCFDD196 pKa = 3.73 DD197 pKa = 3.97 VKK199 pKa = 11.51 YY200 pKa = 11.05 NSPLNNFNKK209 pKa = 10.24 NYY211 pKa = 10.48 YY212 pKa = 10.21 SYY214 pKa = 9.77 NTNDD218 pKa = 3.04 FVSNVV223 pKa = 3.15
Molecular weight: 25.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.044
IPC2_protein 4.24
IPC_protein 4.215
Toseland 4.012
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.088
Rodwell 4.037
Grimsley 3.923
Solomon 4.177
Lehninger 4.139
Nozaki 4.291
DTASelect 4.507
Thurlkill 4.037
EMBOSS 4.101
Sillero 4.329
Patrickios 2.613
IPC_peptide 4.177
IPC2_peptide 4.304
IPC2.peptide.svr19 4.244
Protein with the highest isoelectric point:
>tr|Q9DHR7|Q9DHR7_YLDV 45L protein OS=Yaba-like disease virus OX=132475 GN=45L PE=4 SV=1
MM1 pKa = 7.49 ALSTRR6 pKa = 11.84 EE7 pKa = 3.79 IFSAIGITCMALLMIVSGGALAFKK31 pKa = 10.48 SLAPHH36 pKa = 6.49 RR37 pKa = 11.84 VISMRR42 pKa = 11.84 SVTFNKK48 pKa = 9.88 VITILEE54 pKa = 4.36 YY55 pKa = 9.75 IAILIFVPGTIALYY69 pKa = 9.93 SAYY72 pKa = 9.58 IKK74 pKa = 9.84 TLIVGG79 pKa = 4.05
Molecular weight: 8.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.195
IPC2_protein 9.341
IPC_protein 9.472
Toseland 9.692
ProMoST 9.531
Dawson 9.984
Bjellqvist 9.75
Wikipedia 10.204
Rodwell 10.204
Grimsley 10.087
Solomon 10.072
Lehninger 10.028
Nozaki 9.765
DTASelect 9.721
Thurlkill 9.809
EMBOSS 10.116
Sillero 9.911
Patrickios 7.512
IPC_peptide 10.058
IPC2_peptide 8.668
IPC2.peptide.svr19 8.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
150
0
150
45790
53
1901
305.3
35.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.813 ± 0.121
2.16 ± 0.098
5.337 ± 0.125
5.364 ± 0.141
5.844 ± 0.121
3.542 ± 0.119
1.581 ± 0.081
10.29 ± 0.185
9.777 ± 0.18
9.292 ± 0.179
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.326 ± 0.083
8.812 ± 0.197
2.819 ± 0.095
2.018 ± 0.075
2.669 ± 0.099
7.938 ± 0.164
5.259 ± 0.131
6.283 ± 0.133
0.66 ± 0.044
5.215 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here