Brevibacterium yomogidense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Brevibacteriaceae; Brevibacterium

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2868 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X6XIM2|A0A1X6XIM2_9MICO Glycolate dehydrogenase subunit GlcD OS=Brevibacterium yomogidense OX=946573 GN=FM105_10355 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.74PSRR5 pKa = 11.84ISPRR9 pKa = 11.84TTRR12 pKa = 11.84TIAAAAVLAVTMSGCAGGAGGGTGGGDD39 pKa = 3.16GGGGGTGYY47 pKa = 10.69EE48 pKa = 4.35YY49 pKa = 10.89GASPDD54 pKa = 3.67EE55 pKa = 4.36IKK57 pKa = 10.93AAFADD62 pKa = 4.06VDD64 pKa = 4.36PLTITYY70 pKa = 9.79QPSAQSSEE78 pKa = 4.71GIDD81 pKa = 3.46AYY83 pKa = 10.68RR84 pKa = 11.84AAAFIEE90 pKa = 3.96NLEE93 pKa = 4.23TLSDD97 pKa = 3.67GKK99 pKa = 9.53ITVDD103 pKa = 3.07TTYY106 pKa = 10.73GQGIAGYY113 pKa = 10.39DD114 pKa = 3.94EE115 pKa = 6.03LPDD118 pKa = 3.86ALVDD122 pKa = 3.72GRR124 pKa = 11.84VDD126 pKa = 3.04IAYY129 pKa = 7.33MLPIYY134 pKa = 10.54QPDD137 pKa = 3.6QFPVFHH143 pKa = 6.76AWVGGTTLTGTSPLVDD159 pKa = 3.78EE160 pKa = 5.29LAASAAIGQLTWEE173 pKa = 4.79DD174 pKa = 3.84EE175 pKa = 4.25NLLSEE180 pKa = 5.07FRR182 pKa = 11.84DD183 pKa = 3.6QGIEE187 pKa = 3.7PLTPFNSAGAIMGFCGEE204 pKa = 4.15QHH206 pKa = 6.34VDD208 pKa = 3.09ASDD211 pKa = 3.22WAGGQVRR218 pKa = 11.84ASSQAQITQLNALDD232 pKa = 4.33ASPVSLEE239 pKa = 3.81YY240 pKa = 10.92TEE242 pKa = 4.53TFEE245 pKa = 4.33ALQRR249 pKa = 11.84GTIDD253 pKa = 3.44CTMSAALAAEE263 pKa = 4.21TAGVLEE269 pKa = 4.54VAPHH273 pKa = 5.52ATYY276 pKa = 8.11TTDD279 pKa = 2.74VTFARR284 pKa = 11.84GPGGVYY290 pKa = 10.23AGSAWEE296 pKa = 4.08SWPLAVQQLVFDD308 pKa = 4.02SMEE311 pKa = 4.53DD312 pKa = 3.11EE313 pKa = 4.34FTQARR318 pKa = 11.84RR319 pKa = 11.84GDD321 pKa = 3.41LDD323 pKa = 3.84ANYY326 pKa = 9.97VAAEE330 pKa = 4.17QIRR333 pKa = 11.84EE334 pKa = 4.03QGGSFSEE341 pKa = 4.3MDD343 pKa = 4.04PDD345 pKa = 3.86AQSALLDD352 pKa = 3.8ASQGIVQDD360 pKa = 4.06AAAEE364 pKa = 4.1GHH366 pKa = 6.56LPEE369 pKa = 4.8GTVDD373 pKa = 5.09AIPAAVEE380 pKa = 3.97EE381 pKa = 4.31WRR383 pKa = 11.84GTVEE387 pKa = 3.64EE388 pKa = 5.26LGYY391 pKa = 10.82EE392 pKa = 4.18DD393 pKa = 4.15QGTFADD399 pKa = 3.88YY400 pKa = 11.28DD401 pKa = 3.33EE402 pKa = 5.33WYY404 pKa = 10.33PMDD407 pKa = 4.46DD408 pKa = 3.84EE409 pKa = 5.58EE410 pKa = 4.56YY411 pKa = 9.71LAPVGSTYY419 pKa = 10.4FEE421 pKa = 4.13EE422 pKa = 4.45VMLPHH427 pKa = 6.96RR428 pKa = 11.84PSS430 pKa = 2.95

Molecular weight: 45.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X6WY65|A0A1X6WY65_9MICO Proposed peptidoglycan lipid II flippase MurJ OS=Brevibacterium yomogidense OX=946573 GN=FM105_02150 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 8.12HH4 pKa = 5.4TFQPNNRR11 pKa = 11.84KK12 pKa = 8.98RR13 pKa = 11.84AKK15 pKa = 8.92THH17 pKa = 5.64GFRR20 pKa = 11.84QRR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84RR41 pKa = 11.84ALSAA45 pKa = 3.79

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2868

0

2868

977191

37

1841

340.7

36.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.021 ± 0.075

0.549 ± 0.01

6.813 ± 0.054

6.108 ± 0.048

3.057 ± 0.027

9.165 ± 0.046

2.184 ± 0.025

4.26 ± 0.034

1.891 ± 0.029

9.614 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.041 ± 0.018

1.763 ± 0.024

5.381 ± 0.031

2.891 ± 0.025

7.115 ± 0.058

6.102 ± 0.035

6.233 ± 0.033

8.656 ± 0.044

1.312 ± 0.018

1.844 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski