Changjiang tombus-like virus 16

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG49|A0A1L3KG49_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 16 OX=1922809 PE=4 SV=1
MM1 pKa = 7.39ATFDD5 pKa = 3.66LGAAEE10 pKa = 4.25RR11 pKa = 11.84QLDD14 pKa = 3.8GAIGVISKK22 pKa = 9.93GFKK25 pKa = 10.12LGVMVCGVCTAGYY38 pKa = 9.18LAYY41 pKa = 10.2RR42 pKa = 11.84YY43 pKa = 9.9VPRR46 pKa = 11.84LWRR49 pKa = 11.84LMEE52 pKa = 4.24VMDD55 pKa = 4.97HH56 pKa = 6.47SLTGKK61 pKa = 9.01EE62 pKa = 3.7RR63 pKa = 11.84AMEE66 pKa = 4.18LQSKK70 pKa = 8.97AVEE73 pKa = 4.11YY74 pKa = 11.21VPDD77 pKa = 3.84EE78 pKa = 4.49DD79 pKa = 4.2TDD81 pKa = 3.8EE82 pKa = 4.67EE83 pKa = 4.33EE84 pKa = 4.96ADD86 pKa = 4.66VGAEE90 pKa = 4.78DD91 pKa = 4.44NDD93 pKa = 3.85APPGLGRR100 pKa = 11.84RR101 pKa = 11.84GPAARR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84ARR110 pKa = 11.84KK111 pKa = 8.96EE112 pKa = 3.27KK113 pKa = 8.63HH114 pKa = 5.45TGMDD118 pKa = 4.0GVPLTNYY125 pKa = 8.41YY126 pKa = 11.09ASIVSEE132 pKa = 3.93ARR134 pKa = 11.84VHH136 pKa = 5.96YY137 pKa = 9.72GYY139 pKa = 10.75KK140 pKa = 10.47GYY142 pKa = 10.91SSYY145 pKa = 11.44NAALARR151 pKa = 11.84AYY153 pKa = 8.5MVRR156 pKa = 11.84LMTEE160 pKa = 3.69HH161 pKa = 6.7NVRR164 pKa = 11.84KK165 pKa = 9.79AHH167 pKa = 5.27MLEE170 pKa = 4.04NLEE173 pKa = 4.53DD174 pKa = 3.5MVNAVFAVSAVDD186 pKa = 4.5RR187 pKa = 11.84RR188 pKa = 11.84AQLEE192 pKa = 4.25RR193 pKa = 11.84EE194 pKa = 4.12AGLLFGWMRR203 pKa = 11.84RR204 pKa = 11.84AQNCC208 pKa = 3.24

Molecular weight: 23.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG49|A0A1L3KG49_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 16 OX=1922809 PE=4 SV=1
MM1 pKa = 7.73AKK3 pKa = 10.11NKK5 pKa = 10.26KK6 pKa = 10.1SDD8 pKa = 3.59VKK10 pKa = 10.57KK11 pKa = 10.96SKK13 pKa = 10.89AGTSKK18 pKa = 10.05YY19 pKa = 8.71QKK21 pKa = 10.14KK22 pKa = 10.19AFGMYY27 pKa = 9.6KK28 pKa = 9.92QIEE31 pKa = 4.63AGTLDD36 pKa = 3.59RR37 pKa = 11.84AALEE41 pKa = 4.13AAVMYY46 pKa = 10.41SDD48 pKa = 4.06PCGADD53 pKa = 3.33LVPTVYY59 pKa = 10.41PGDD62 pKa = 3.48AGYY65 pKa = 10.48INRR68 pKa = 11.84FNTINTVGTVAGQTATVMIWRR89 pKa = 11.84PGTQGCVEE97 pKa = 4.32YY98 pKa = 10.67GVADD102 pKa = 3.74GGVGVVTAYY111 pKa = 7.93NTNFPGLTFLNTNASKK127 pKa = 9.32TRR129 pKa = 11.84CGAFCATVRR138 pKa = 11.84PVAAPNTCTGTIYY151 pKa = 10.64FGVVNARR158 pKa = 11.84AVRR161 pKa = 11.84SGLNLSPNAMIPLCSEE177 pKa = 4.29SVSASQALMAPLEE190 pKa = 4.43VRR192 pKa = 11.84WSPGDD197 pKa = 3.19FDD199 pKa = 4.46SRR201 pKa = 11.84YY202 pKa = 10.44SEE204 pKa = 4.21VNNPNVAADD213 pKa = 3.87DD214 pKa = 3.8SDD216 pKa = 4.56YY217 pKa = 11.84NCLLVVAIGLPAATGIQYY235 pKa = 10.33RR236 pKa = 11.84ATSIMEE242 pKa = 3.99WCPNSNLGIANDD254 pKa = 3.67ATRR257 pKa = 11.84VKK259 pKa = 10.49PSACDD264 pKa = 3.49KK265 pKa = 10.64EE266 pKa = 4.82CVLNFLKK273 pKa = 10.82KK274 pKa = 9.68KK275 pKa = 10.49DD276 pKa = 3.94KK277 pKa = 10.68NWWWSLGNKK286 pKa = 6.09TLKK289 pKa = 9.57VAKK292 pKa = 8.81TVASGYY298 pKa = 9.92YY299 pKa = 9.54SGGAIGALGSLTKK312 pKa = 10.52FMM314 pKa = 5.62

Molecular weight: 33.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1021

208

499

340.3

37.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.696 ± 1.605

2.351 ± 0.331

4.995 ± 0.243

5.387 ± 1.091

3.134 ± 0.415

8.227 ± 0.459

1.567 ± 0.561

3.232 ± 0.503

5.093 ± 0.585

9.403 ± 1.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.036 ± 0.623

4.016 ± 1.104

4.505 ± 0.454

3.232 ± 0.761

7.15 ± 1.317

6.17 ± 0.641

5.191 ± 0.894

8.423 ± 0.115

1.469 ± 0.179

3.722 ± 0.554
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski