Salmonella phage 19

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 194 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N6WGI9|A0A0N6WGI9_9CAUD RNA polymerase binding protein OS=Salmonella phage 19 OX=1654883 GN=SP19_178 PE=4 SV=1
MM1 pKa = 7.57ASDD4 pKa = 3.88VCQYY8 pKa = 11.84DD9 pKa = 4.0DD10 pKa = 4.78VEE12 pKa = 5.56PDD14 pKa = 3.67LMLSGAKK21 pKa = 9.35RR22 pKa = 11.84DD23 pKa = 3.62CDD25 pKa = 3.96FNLVEE30 pKa = 4.8IYY32 pKa = 10.72DD33 pKa = 4.12WFLSIPDD40 pKa = 3.69QKK42 pKa = 10.85EE43 pKa = 3.98GRR45 pKa = 11.84CAFF48 pKa = 3.97

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N6WGH8|A0A0N6WGH8_9CAUD Uncharacterized protein OS=Salmonella phage 19 OX=1654883 GN=SP19_68 PE=4 SV=1
MM1 pKa = 7.37FKK3 pKa = 10.72SCFGRR8 pKa = 11.84LNRR11 pKa = 11.84HH12 pKa = 4.48RR13 pKa = 11.84TEE15 pKa = 4.42KK16 pKa = 10.39ILRR19 pKa = 11.84SKK21 pKa = 10.04MMQGDD26 pKa = 3.8SAAYY30 pKa = 5.31TTNTGSQQSVSLTT43 pKa = 3.46

Molecular weight: 4.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

194

0

194

13346

37

639

68.8

7.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.961 ± 0.29

1.701 ± 0.178

5.919 ± 0.224

5.26 ± 0.283

3.971 ± 0.245

6.819 ± 0.608

1.888 ± 0.142

5.14 ± 0.211

5.305 ± 0.272

8.887 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.889 ± 0.208

4.406 ± 0.245

4.046 ± 0.27

4.226 ± 0.218

5.32 ± 0.277

7.231 ± 0.277

6.152 ± 0.227

7.65 ± 0.272

1.918 ± 0.121

3.312 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski