Klebsiella phage vB_Kp3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0IK53|A0A0P0IK53_9CAUD Exonuclease OS=Klebsiella phage vB_Kp3 OX=1701806 PE=4 SV=1
MM1 pKa = 7.15EE2 pKa = 5.76AAPKK6 pKa = 10.36ALTAAIEE13 pKa = 4.17GLYY16 pKa = 10.67FPDD19 pKa = 5.25PPQQLVDD26 pKa = 3.62NFGGEE31 pKa = 4.17LEE33 pKa = 4.38DD34 pKa = 3.79YY35 pKa = 10.34RR36 pKa = 11.84PEE38 pKa = 4.08VEE40 pKa = 4.81LSAEE44 pKa = 4.23IVDD47 pKa = 4.03SFYY50 pKa = 10.54FYY52 pKa = 11.26QRR54 pKa = 11.84IDD56 pKa = 3.72TQWRR60 pKa = 11.84TGPGGLIGLDD70 pKa = 3.43YY71 pKa = 11.42NVIPVMADD79 pKa = 3.42LYY81 pKa = 10.88GVKK84 pKa = 9.97FDD86 pKa = 4.68RR87 pKa = 11.84LLMDD91 pKa = 5.48DD92 pKa = 4.25LAIMEE97 pKa = 4.4GAAIRR102 pKa = 11.84VLMSRR107 pKa = 11.84SDD109 pKa = 3.39TAA111 pKa = 3.84

Molecular weight: 12.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0IXW2|A0A0P0IXW2_9CAUD Uncharacterized protein OS=Klebsiella phage vB_Kp3 OX=1701806 PE=4 SV=1
MM1 pKa = 7.39SVLIIWCVMAVLVGIAADD19 pKa = 3.62RR20 pKa = 11.84RR21 pKa = 11.84GRR23 pKa = 11.84SGLGWSLLALLISPLLAILFLLVMRR48 pKa = 11.84NIAAEE53 pKa = 4.01EE54 pKa = 3.62EE55 pKa = 3.93RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84IEE60 pKa = 3.82EE61 pKa = 3.66RR62 pKa = 11.84RR63 pKa = 11.84HH64 pKa = 5.72IEE66 pKa = 3.63TIAAIRR72 pKa = 11.84GNKK75 pKa = 7.78EE76 pKa = 3.32

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

15356

55

3358

260.3

28.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.139 ± 0.493

1.042 ± 0.176

6.206 ± 0.193

5.685 ± 0.537

3.504 ± 0.271

7.912 ± 0.357

1.472 ± 0.23

5.946 ± 0.196

5.425 ± 0.424

6.909 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.82 ± 0.301

4.93 ± 0.398

3.666 ± 0.331

4.591 ± 0.308

5.757 ± 0.35

6.095 ± 0.614

6.558 ± 0.789

6.714 ± 0.227

1.537 ± 0.112

3.093 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski