Natronomonas sp. CBA1133

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Natronomonas; unclassified Natronomonas

Average proteome isoelectric point is 4.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2582 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M9VVT8|A0A3M9VVT8_9EURY Uncharacterized protein OS=Natronomonas sp. CBA1133 OX=2487041 GN=Nmn1133_09815 PE=4 SV=1
MM1 pKa = 7.04VQTDD5 pKa = 4.04SDD7 pKa = 4.06SQLQRR12 pKa = 11.84GDD14 pKa = 3.68LAPDD18 pKa = 3.71FRR20 pKa = 11.84LVGTEE25 pKa = 4.05GAHH28 pKa = 4.69YY29 pKa = 9.49TPDD32 pKa = 3.31SFADD36 pKa = 3.4NDD38 pKa = 3.67ALLVVFTCNHH48 pKa = 5.68CPYY51 pKa = 10.32AKK53 pKa = 10.56AKK55 pKa = 9.01FAPLNAIAEE64 pKa = 4.5EE65 pKa = 4.26YY66 pKa = 10.64DD67 pKa = 3.29DD68 pKa = 4.48CAVVGINPNDD78 pKa = 3.79ADD80 pKa = 4.35EE81 pKa = 5.09YY82 pKa = 10.65PDD84 pKa = 4.12DD85 pKa = 4.39SYY87 pKa = 12.04EE88 pKa = 4.15RR89 pKa = 11.84MCEE92 pKa = 3.91LVDD95 pKa = 5.28DD96 pKa = 4.38GTIGYY101 pKa = 8.47DD102 pKa = 3.06AYY104 pKa = 11.37LRR106 pKa = 11.84DD107 pKa = 4.19DD108 pKa = 4.7DD109 pKa = 5.34GSVAEE114 pKa = 5.28AYY116 pKa = 10.08GAVCTPDD123 pKa = 3.18PFLFARR129 pKa = 11.84EE130 pKa = 3.97DD131 pKa = 4.11GEE133 pKa = 4.13WRR135 pKa = 11.84LAYY138 pKa = 9.98HH139 pKa = 6.71GRR141 pKa = 11.84LDD143 pKa = 4.79DD144 pKa = 5.24ALNPDD149 pKa = 5.0DD150 pKa = 5.07EE151 pKa = 5.05PSGEE155 pKa = 4.02PGFEE159 pKa = 3.54VRR161 pKa = 11.84QAIDD165 pKa = 3.23AVLSGEE171 pKa = 4.4DD172 pKa = 3.97VSLEE176 pKa = 4.21DD177 pKa = 3.85NPSRR181 pKa = 11.84GCSIKK186 pKa = 10.16WPSAA190 pKa = 3.13

Molecular weight: 20.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M9VGD9|A0A3M9VGD9_9EURY Uncharacterized protein OS=Natronomonas sp. CBA1133 OX=2487041 GN=Nmn1133_12940 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.44SKK4 pKa = 9.2STRR7 pKa = 11.84SGIGRR12 pKa = 11.84VPSIFFATPTGISAPTRR29 pKa = 11.84SGQYY33 pKa = 9.12ATTSTAWKK41 pKa = 9.79LRR43 pKa = 11.84RR44 pKa = 11.84KK45 pKa = 9.0PVSPPNPLPSCRR57 pKa = 11.84GTTSKK62 pKa = 10.7SICGRR67 pKa = 11.84STTSKK72 pKa = 9.37STASPHH78 pKa = 5.1QRR80 pKa = 11.84LASGQFFARR89 pKa = 11.84TKK91 pKa = 8.53PVKK94 pKa = 9.98FVRR97 pKa = 11.84SFRR100 pKa = 11.84SCATLSPRR108 pKa = 11.84SMRR111 pKa = 11.84STASGSRR118 pKa = 11.84RR119 pKa = 11.84PFSRR123 pKa = 11.84APRR126 pKa = 11.84PSWHH130 pKa = 6.75SIRR133 pKa = 11.84PIRR136 pKa = 11.84HH137 pKa = 5.63RR138 pKa = 11.84GSRR141 pKa = 11.84RR142 pKa = 11.84PLTLVV147 pKa = 3.05

Molecular weight: 16.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2582

0

2582

718440

42

1687

278.2

30.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.147 ± 0.074

0.733 ± 0.016

8.294 ± 0.056

8.431 ± 0.07

3.301 ± 0.029

8.432 ± 0.048

2.003 ± 0.023

3.941 ± 0.041

1.789 ± 0.027

9.028 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.713 ± 0.02

2.309 ± 0.027

4.655 ± 0.03

2.677 ± 0.03

6.608 ± 0.048

5.441 ± 0.041

6.852 ± 0.041

8.898 ± 0.054

1.06 ± 0.017

2.686 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski