Momordica charantia associated gemycircularvirus
Average proteome isoelectric point is 7.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345S7U9|A0A345S7U9_9VIRU Replication-associated protein OS=Momordica charantia associated gemycircularvirus OX=2291613 PE=3 SV=1
MM1 pKa = 7.84 PSFVCNFRR9 pKa = 11.84 YY10 pKa = 10.28 GLITYY15 pKa = 7.3 PQCDD19 pKa = 3.52 GLDD22 pKa = 3.43 PFRR25 pKa = 11.84 VMEE28 pKa = 4.46 RR29 pKa = 11.84 FSSLGAEE36 pKa = 4.35 CIIGRR41 pKa = 11.84 EE42 pKa = 3.83 RR43 pKa = 11.84 HH44 pKa = 5.72 EE45 pKa = 5.01 DD46 pKa = 3.46 GGLHH50 pKa = 5.74 LHH52 pKa = 6.24 VFADD56 pKa = 4.7 FGRR59 pKa = 11.84 KK60 pKa = 8.14 FRR62 pKa = 11.84 SRR64 pKa = 11.84 KK65 pKa = 7.79 TDD67 pKa = 2.98 VFDD70 pKa = 3.81 VDD72 pKa = 3.65 GRR74 pKa = 11.84 HH75 pKa = 6.28 PNVEE79 pKa = 4.04 PSKK82 pKa = 9.22 GTPEE86 pKa = 3.79 KK87 pKa = 10.85 GYY89 pKa = 10.88 DD90 pKa = 3.47 YY91 pKa = 10.69 AIKK94 pKa = 10.83 DD95 pKa = 3.47 GDD97 pKa = 4.33 VVCGGLGRR105 pKa = 11.84 PEE107 pKa = 3.97 PSGGGDD113 pKa = 3.43 GASSAKK119 pKa = 8.61 WARR122 pKa = 11.84 ITQAEE127 pKa = 4.11 NRR129 pKa = 11.84 DD130 pKa = 3.9 EE131 pKa = 4.33 FWEE134 pKa = 4.38 LVHH137 pKa = 7.01 EE138 pKa = 5.14 LDD140 pKa = 4.73 PKK142 pKa = 10.9 AAACSFNALSKK153 pKa = 9.33 YY154 pKa = 10.43 ADD156 pKa = 3.0 WRR158 pKa = 11.84 FAEE161 pKa = 4.33 VPPEE165 pKa = 3.98 YY166 pKa = 10.36 EE167 pKa = 3.34 HH168 pKa = 7.5 DD169 pKa = 3.61 GRR171 pKa = 11.84 IEE173 pKa = 4.01 FVPGDD178 pKa = 3.59 ADD180 pKa = 4.72 GRR182 pKa = 11.84 ADD184 pKa = 3.28 WVSQSGIRR192 pKa = 11.84 LAEE195 pKa = 4.12 PFLAGTRR202 pKa = 11.84 VKK204 pKa = 10.87 SLVLWGKK211 pKa = 10.19 SRR213 pKa = 11.84 TGKK216 pKa = 6.05 TTWARR221 pKa = 11.84 SLGPHH226 pKa = 6.31 VYY228 pKa = 10.18 CVGLVSGTEE237 pKa = 4.02 CAKK240 pKa = 10.98 GLDD243 pKa = 3.16 ARR245 pKa = 11.84 YY246 pKa = 9.64 AVFDD250 pKa = 5.19 DD251 pKa = 3.36 IRR253 pKa = 11.84 GGLGFFHH260 pKa = 7.61 GYY262 pKa = 9.93 KK263 pKa = 9.57 EE264 pKa = 3.99 WLGAQPHH271 pKa = 5.37 VPIKK275 pKa = 10.13 EE276 pKa = 4.14 LYY278 pKa = 9.69 RR279 pKa = 11.84 EE280 pKa = 4.04 PRR282 pKa = 11.84 YY283 pKa = 9.43 MRR285 pKa = 11.84 WNKK288 pKa = 9.74 PSIWVCNRR296 pKa = 11.84 DD297 pKa = 3.62 PRR299 pKa = 11.84 LEE301 pKa = 4.31 AYY303 pKa = 8.55 PPNATPDD310 pKa = 3.91 WEE312 pKa = 4.27 WMDD315 pKa = 4.74 DD316 pKa = 3.47 NVDD319 pKa = 4.4 FIEE322 pKa = 4.46 VTDD325 pKa = 4.0 SLIASISHH333 pKa = 7.17 ASTEE337 pKa = 4.06
Molecular weight: 37.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.342
IPC2_protein 5.347
IPC_protein 5.347
Toseland 5.512
ProMoST 5.486
Dawson 5.461
Bjellqvist 5.55
Wikipedia 5.41
Rodwell 5.41
Grimsley 5.55
Solomon 5.461
Lehninger 5.436
Nozaki 5.652
DTASelect 5.83
Thurlkill 5.664
EMBOSS 5.626
Sillero 5.741
Patrickios 4.062
IPC_peptide 5.474
IPC2_peptide 5.741
IPC2.peptide.svr19 5.715
Protein with the highest isoelectric point:
>tr|A0A345S7U9|A0A345S7U9_9VIRU Replication-associated protein OS=Momordica charantia associated gemycircularvirus OX=2291613 PE=3 SV=1
MM1 pKa = 7.95 AYY3 pKa = 9.64 RR4 pKa = 11.84 RR5 pKa = 11.84 SVRR8 pKa = 11.84 RR9 pKa = 11.84 APARR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 PTRR18 pKa = 11.84 KK19 pKa = 8.77 SSYY22 pKa = 7.52 GSRR25 pKa = 11.84 YY26 pKa = 8.58 RR27 pKa = 11.84 RR28 pKa = 11.84 SYY30 pKa = 7.13 TAKK33 pKa = 10.15 RR34 pKa = 11.84 RR35 pKa = 11.84 FTRR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 8.93 RR41 pKa = 11.84 PMSRR45 pKa = 11.84 KK46 pKa = 9.14 SLLNVTSRR54 pKa = 11.84 KK55 pKa = 9.88 KK56 pKa = 9.81 QDD58 pKa = 2.94 TMLGYY63 pKa = 11.12 SNLANDD69 pKa = 4.13 STDD72 pKa = 2.91 GSNKK76 pKa = 8.33 FHH78 pKa = 7.02 SGAGILKK85 pKa = 9.23 GGRR88 pKa = 11.84 MYY90 pKa = 10.49 IIPWIATARR99 pKa = 11.84 DD100 pKa = 3.65 LSPTDD105 pKa = 3.44 GTLGTVAQKK114 pKa = 10.95 ASRR117 pKa = 11.84 TSTEE121 pKa = 3.71 CYY123 pKa = 9.18 MRR125 pKa = 11.84 GLRR128 pKa = 11.84 EE129 pKa = 4.33 RR130 pKa = 11.84 IQIQTNNGTSWQWRR144 pKa = 11.84 RR145 pKa = 11.84 ICFTFKK151 pKa = 11.06 GPLLVNATTGSEE163 pKa = 3.99 TQRR166 pKa = 11.84 YY167 pKa = 8.48 QIEE170 pKa = 4.48 TNQGFARR177 pKa = 11.84 VMSDD181 pKa = 3.61 FALGTAADD189 pKa = 4.26 PLNGVLFKK197 pKa = 11.03 GLQSVDD203 pKa = 2.37 WSSYY207 pKa = 7.47 FTAKK211 pKa = 9.67 TDD213 pKa = 3.38 NSLVNIMYY221 pKa = 9.37 DD222 pKa = 3.01 QTRR225 pKa = 11.84 IISSGNASGVMRR237 pKa = 11.84 NFKK240 pKa = 9.79 LWHH243 pKa = 6.22 PMNKK247 pKa = 9.33 NLYY250 pKa = 8.36 YY251 pKa = 10.85 NEE253 pKa = 4.64 DD254 pKa = 3.89 EE255 pKa = 4.58 IGATTGTSVYY265 pKa = 9.16 STSSKK270 pKa = 10.77 RR271 pKa = 11.84 GMGDD275 pKa = 3.32 YY276 pKa = 10.78 YY277 pKa = 11.59 VVDD280 pKa = 5.52 FILAGTGSNINDD292 pKa = 3.41 LMSFEE297 pKa = 5.22 PDD299 pKa = 2.85 STLYY303 pKa = 8.55 WHH305 pKa = 6.72 EE306 pKa = 4.03 RR307 pKa = 3.41
Molecular weight: 34.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.809
IPC_protein 10.511
Toseland 10.365
ProMoST 10.204
Dawson 10.555
Bjellqvist 10.292
Wikipedia 10.789
Rodwell 10.789
Grimsley 10.643
Solomon 10.613
Lehninger 10.57
Nozaki 10.35
DTASelect 10.292
Thurlkill 10.409
EMBOSS 10.774
Sillero 10.482
Patrickios 10.35
IPC_peptide 10.613
IPC2_peptide 9.194
IPC2.peptide.svr19 8.612
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
644
307
337
322.0
36.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.988 ± 0.566
1.553 ± 0.639
6.522 ± 1.159
4.969 ± 1.675
4.348 ± 0.542
9.161 ± 0.722
2.019 ± 0.738
4.193 ± 0.261
4.658 ± 0.161
6.366 ± 0.105
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.329 ± 0.889
4.037 ± 1.294
4.814 ± 1.334
2.174 ± 0.768
9.006 ± 1.004
8.23 ± 1.555
6.366 ± 2.183
5.28 ± 0.971
2.64 ± 0.486
4.348 ± 0.612
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here