Streptococcus phage Javan616

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BFZ8|A0A4D6BFZ8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan616 OX=2548282 GN=Javan616_0044 PE=4 SV=1
MM1 pKa = 7.7IDD3 pKa = 3.83GYY5 pKa = 9.22TEE7 pKa = 4.13GGLWIDD13 pKa = 4.23DD14 pKa = 3.79NYY16 pKa = 11.44KK17 pKa = 10.27IIQRR21 pKa = 11.84LSFEE25 pKa = 4.31YY26 pKa = 10.78GGLSGIKK33 pKa = 9.07GKK35 pKa = 10.1YY36 pKa = 9.42RR37 pKa = 11.84IEE39 pKa = 3.79IDD41 pKa = 3.27IEE43 pKa = 4.12EE44 pKa = 4.35VEE46 pKa = 4.15

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BCJ9|A0A4D6BCJ9_9CAUD Uncharacterized protein OS=Streptococcus phage Javan616 OX=2548282 GN=Javan616_0027 PE=4 SV=1
MM1 pKa = 7.04VRR3 pKa = 11.84QKK5 pKa = 10.93GRR7 pKa = 11.84EE8 pKa = 3.87AQVAVEE14 pKa = 4.36SEE16 pKa = 4.45LNRR19 pKa = 11.84SSLRR23 pKa = 11.84VEE25 pKa = 4.72RR26 pKa = 11.84KK27 pKa = 9.85AKK29 pKa = 10.04IYY31 pKa = 10.7APWDD35 pKa = 3.67TGWMSNSIYY44 pKa = 11.3SMYY47 pKa = 10.62QDD49 pKa = 3.22VLKK52 pKa = 10.95YY53 pKa = 10.58VVVSPAEE60 pKa = 3.62YY61 pKa = 10.16SIYY64 pKa = 10.3VEE66 pKa = 3.87KK67 pKa = 10.08GTRR70 pKa = 11.84KK71 pKa = 9.12MMAQPFIHH79 pKa = 7.08PALQEE84 pKa = 3.84EE85 pKa = 4.82APILMKK91 pKa = 10.59RR92 pKa = 11.84LSKK95 pKa = 10.11MFRR98 pKa = 11.84KK99 pKa = 9.77

Molecular weight: 11.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

11958

45

1575

209.8

23.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.459 ± 0.852

0.477 ± 0.149

5.854 ± 0.463

7.342 ± 0.769

3.621 ± 0.164

5.879 ± 0.465

1.455 ± 0.202

7.041 ± 0.268

8.145 ± 0.521

8.187 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.256

6.13 ± 0.202

2.45 ± 0.216

4.19 ± 0.295

4.198 ± 0.354

7.209 ± 0.744

6.757 ± 0.406

6.205 ± 0.198

0.82 ± 0.103

3.939 ± 0.442
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski