Amino acid dipepetide frequency for Lewinella sp. 4G2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.271AlaAla: 9.271 ± 0.14	0.829AlaCys: 0.829 ± 0.029	5.869AlaAsp: 5.869 ± 0.115	5.591AlaGlu: 5.591 ± 0.079	3.508AlaPhe: 3.508 ± 0.056	8.117AlaGly: 8.117 ± 0.115	1.29AlaHis: 1.29 ± 0.038	4.856AlaIle: 4.856 ± 0.075	3.471AlaLys: 3.471 ± 0.072	7.894AlaLeu: 7.894 ± 0.137	1.851AlaMet: 1.851 ± 0.045	3.78AlaAsn: 3.78 ± 0.075	3.866AlaPro: 3.866 ± 0.071	3.073AlaGln: 3.073 ± 0.049	4.314AlaArg: 4.314 ± 0.078	4.813AlaSer: 4.813 ± 0.062	6.278AlaThr: 6.278 ± 0.16	5.502AlaVal: 5.502 ± 0.086	1.004AlaTrp: 1.004 ± 0.031	2.99AlaTyr: 2.99 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.742CysAla: 0.742 ± 0.036	0.129CysCys: 0.129 ± 0.01	0.63CysAsp: 0.63 ± 0.051	0.527CysGlu: 0.527 ± 0.03	0.395CysPhe: 0.395 ± 0.016	0.861CysGly: 0.861 ± 0.043	0.19CysHis: 0.19 ± 0.012	0.397CysIle: 0.397 ± 0.018	0.239CysLys: 0.239 ± 0.013	0.781CysLeu: 0.781 ± 0.034	0.14CysMet: 0.14 ± 0.008	0.411CysAsn: 0.411 ± 0.03	0.517CysPro: 0.517 ± 0.038	0.3CysGln: 0.3 ± 0.017	0.403CysArg: 0.403 ± 0.02	0.645CysSer: 0.645 ± 0.036	0.668CysThr: 0.668 ± 0.043	0.543CysVal: 0.543 ± 0.029	0.092CysTrp: 0.092 ± 0.009	0.26CysTyr: 0.26 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	5.645AspAla: 5.645 ± 0.224	0.641AspCys: 0.641 ± 0.046	4.098AspAsp: 4.098 ± 0.171	4.149AspGlu: 4.149 ± 0.081	3.297AspPhe: 3.297 ± 0.054	6.275AspGly: 6.275 ± 0.198	1.172AspHis: 1.172 ± 0.037	3.241AspIle: 3.241 ± 0.055	2.109AspLys: 2.109 ± 0.056	6.624AspLeu: 6.624 ± 0.079	1.112AspMet: 1.112 ± 0.047	2.799AspAsn: 2.799 ± 0.099	3.163AspPro: 3.163 ± 0.077	2.33AspGln: 2.33 ± 0.052	3.727AspArg: 3.727 ± 0.07	2.939AspSer: 2.939 ± 0.073	3.228AspThr: 3.228 ± 0.13	4.415AspVal: 4.415 ± 0.092	0.957AspTrp: 0.957 ± 0.03	2.712AspTyr: 2.712 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	5.576GluAla: 5.576 ± 0.081	0.397GluCys: 0.397 ± 0.029	3.87GluAsp: 3.87 ± 0.077	4.531GluGlu: 4.531 ± 0.075	2.553GluPhe: 2.553 ± 0.07	4.523GluGly: 4.523 ± 0.067	1.015GluHis: 1.015 ± 0.033	3.623GluIle: 3.623 ± 0.052	2.693GluLys: 2.693 ± 0.064	6.572GluLeu: 6.572 ± 0.094	1.657GluMet: 1.657 ± 0.036	2.727GluAsn: 2.727 ± 0.056	2.319GluPro: 2.319 ± 0.056	2.312GluGln: 2.312 ± 0.051	3.892GluArg: 3.892 ± 0.076	2.856GluSer: 2.856 ± 0.047	3.546GluThr: 3.546 ± 0.084	4.835GluVal: 4.835 ± 0.061	0.759GluTrp: 0.759 ± 0.025	2.085GluTyr: 2.085 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	3.788PheAla: 3.788 ± 0.08	0.413PheCys: 0.413 ± 0.03	3.069PheAsp: 3.069 ± 0.076	2.519PheGlu: 2.519 ± 0.052	2.123PhePhe: 2.123 ± 0.049	3.807PheGly: 3.807 ± 0.073	0.727PheHis: 0.727 ± 0.025	2.134PheIle: 2.134 ± 0.05	1.495PheLys: 1.495 ± 0.04	4.108PheLeu: 4.108 ± 0.081	0.775PheMet: 0.775 ± 0.026	2.128PheAsn: 2.128 ± 0.044	1.904PhePro: 1.904 ± 0.043	1.428PheGln: 1.428 ± 0.037	2.471PheArg: 2.471 ± 0.056	3.13PheSer: 3.13 ± 0.051	3.594PheThr: 3.594 ± 0.087	2.953PheVal: 2.953 ± 0.062	0.526PheTrp: 0.526 ± 0.018	1.549PheTyr: 1.549 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	6.456GlyAla: 6.456 ± 0.098	0.983GlyCys: 0.983 ± 0.063	5.162GlyAsp: 5.162 ± 0.169	5.215GlyGlu: 5.215 ± 0.087	3.592GlyPhe: 3.592 ± 0.053	6.967GlyGly: 6.967 ± 0.121	1.37GlyHis: 1.37 ± 0.038	4.61GlyIle: 4.61 ± 0.081	3.728GlyLys: 3.728 ± 0.08	7.226GlyLeu: 7.226 ± 0.089	1.843GlyMet: 1.843 ± 0.043	3.806GlyAsn: 3.806 ± 0.094	2.684GlyPro: 2.684 ± 0.055	3.022GlyGln: 3.022 ± 0.052	4.291GlyArg: 4.291 ± 0.077	4.811GlySer: 4.811 ± 0.078	5.768GlyThr: 5.768 ± 0.161	5.317GlyVal: 5.317 ± 0.088	1.042GlyTrp: 1.042 ± 0.032	2.896GlyTyr: 2.896 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.275HisAla: 1.275 ± 0.039	0.17HisCys: 0.17 ± 0.011	0.932HisAsp: 0.932 ± 0.031	0.921HisGlu: 0.921 ± 0.032	0.982HisPhe: 0.982 ± 0.031	1.249HisGly: 1.249 ± 0.041	0.522HisHis: 0.522 ± 0.022	0.774HisIle: 0.774 ± 0.027	0.556HisLys: 0.556 ± 0.022	2.029HisLeu: 2.029 ± 0.051	0.232HisMet: 0.232 ± 0.014	0.611HisAsn: 0.611 ± 0.023	1.106HisPro: 1.106 ± 0.031	0.676HisGln: 0.676 ± 0.024	1.136HisArg: 1.136 ± 0.035	0.801HisSer: 0.801 ± 0.03	0.858HisThr: 0.858 ± 0.028	1.068HisVal: 1.068 ± 0.032	0.259HisTrp: 0.259 ± 0.013	0.808HisTyr: 0.808 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	4.698IleAla: 4.698 ± 0.07	0.543IleCys: 0.543 ± 0.028	3.971IleAsp: 3.971 ± 0.059	3.5IleGlu: 3.5 ± 0.059	2.234IlePhe: 2.234 ± 0.05	4.252IleGly: 4.252 ± 0.066	1.024IleHis: 1.024 ± 0.033	3.104IleIle: 3.104 ± 0.064	2.266IleLys: 2.266 ± 0.055	4.662IleLeu: 4.662 ± 0.075	0.957IleMet: 0.957 ± 0.031	2.76IleAsn: 2.76 ± 0.054	2.576IlePro: 2.576 ± 0.059	1.778IleGln: 1.778 ± 0.039	2.826IleArg: 2.826 ± 0.054	3.565IleSer: 3.565 ± 0.053	4.051IleThr: 4.051 ± 0.113	3.743IleVal: 3.743 ± 0.06	0.516IleTrp: 0.516 ± 0.021	1.844IleTyr: 1.844 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	3.464LysAla: 3.464 ± 0.075	0.187LysCys: 0.187 ± 0.011	2.33LysAsp: 2.33 ± 0.055	2.648LysGlu: 2.648 ± 0.067	1.474LysPhe: 1.474 ± 0.038	2.68LysGly: 2.68 ± 0.053	0.719LysHis: 0.719 ± 0.025	2.286LysIle: 2.286 ± 0.059	2.226LysLys: 2.226 ± 0.064	3.804LysLeu: 3.804 ± 0.085	1.184LysMet: 1.184 ± 0.032	1.586LysAsn: 1.586 ± 0.043	1.635LysPro: 1.635 ± 0.042	1.478LysGln: 1.478 ± 0.039	2.32LysArg: 2.32 ± 0.055	2.186LysSer: 2.186 ± 0.055	2.192LysThr: 2.192 ± 0.046	2.781LysVal: 2.781 ± 0.062	0.518LysTrp: 0.518 ± 0.019	1.47LysTyr: 1.47 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	8.791LeuAla: 8.791 ± 0.125	0.823LeuCys: 0.823 ± 0.026	6.047LeuAsp: 6.047 ± 0.089	5.869LeuGlu: 5.869 ± 0.083	4.2LeuPhe: 4.2 ± 0.09	6.839LeuGly: 6.839 ± 0.103	1.657LeuHis: 1.657 ± 0.051	4.995LeuIle: 4.995 ± 0.093	3.976LeuLys: 3.976 ± 0.087	10.09LeuLeu: 10.09 ± 0.16	1.796LeuMet: 1.796 ± 0.042	4.336LeuAsn: 4.336 ± 0.067	5.155LeuPro: 5.155 ± 0.071	3.315LeuGln: 3.315 ± 0.068	6.054LeuArg: 6.054 ± 0.104	6.4LeuSer: 6.4 ± 0.087	6.425LeuThr: 6.425 ± 0.094	6.095LeuVal: 6.095 ± 0.084	0.902LeuTrp: 0.902 ± 0.028	3.053LeuTyr: 3.053 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	1.928MetAla: 1.928 ± 0.045	0.126MetCys: 0.126 ± 0.008	1.296MetAsp: 1.296 ± 0.044	1.241MetGlu: 1.241 ± 0.036	0.583MetPhe: 0.583 ± 0.021	1.462MetGly: 1.462 ± 0.036	0.335MetHis: 0.335 ± 0.018	1.161MetIle: 1.161 ± 0.03	1.052MetLys: 1.052 ± 0.035	1.819MetLeu: 1.819 ± 0.044	0.493MetMet: 0.493 ± 0.02	0.878MetAsn: 0.878 ± 0.029	0.99MetPro: 0.99 ± 0.029	0.752MetGln: 0.752 ± 0.022	1.229MetArg: 1.229 ± 0.034	1.192MetSer: 1.192 ± 0.031	1.351MetThr: 1.351 ± 0.04	1.402MetVal: 1.402 ± 0.044	0.154MetTrp: 0.154 ± 0.01	0.493MetTyr: 0.493 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.432AsnAla: 3.432 ± 0.059	0.483AsnCys: 0.483 ± 0.039	2.879AsnAsp: 2.879 ± 0.083	2.465AsnGlu: 2.465 ± 0.045	2.134AsnPhe: 2.134 ± 0.054	4.248AsnGly: 4.248 ± 0.134	0.683AsnHis: 0.683 ± 0.023	2.372AsnIle: 2.372 ± 0.046	1.514AsnLys: 1.514 ± 0.041	4.34AsnLeu: 4.34 ± 0.059	0.743AsnMet: 0.743 ± 0.028	2.074AsnAsn: 2.074 ± 0.061	2.641AsnPro: 2.641 ± 0.052	1.63AsnGln: 1.63 ± 0.04	2.353AsnArg: 2.353 ± 0.051	2.468AsnSer: 2.468 ± 0.054	2.468AsnThr: 2.468 ± 0.052	3.034AsnVal: 3.034 ± 0.059	0.684AsnTrp: 0.684 ± 0.023	1.935AsnTyr: 1.935 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	4.767ProAla: 4.767 ± 0.074	0.278ProCys: 0.278 ± 0.021	3.327ProAsp: 3.327 ± 0.077	3.382ProGlu: 3.382 ± 0.062	2.013ProPhe: 2.013 ± 0.04	4.121ProGly: 4.121 ± 0.101	0.648ProHis: 0.648 ± 0.026	2.453ProIle: 2.453 ± 0.045	1.591ProLys: 1.591 ± 0.042	3.796ProLeu: 3.796 ± 0.066	0.756ProMet: 0.756 ± 0.024	2.304ProAsn: 2.304 ± 0.048	1.821ProPro: 1.821 ± 0.064	1.42ProGln: 1.42 ± 0.033	1.834ProArg: 1.834 ± 0.045	2.6ProSer: 2.6 ± 0.049	3.201ProThr: 3.201 ± 0.054	3.442ProVal: 3.442 ± 0.068	0.473ProTrp: 0.473 ± 0.019	1.472ProTyr: 1.472 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.899GlnAla: 2.899 ± 0.058	0.212GlnCys: 0.212 ± 0.016	2.124GlnAsp: 2.124 ± 0.06	2.132GlnGlu: 2.132 ± 0.049	1.627GlnPhe: 1.627 ± 0.032	2.223GlnGly: 2.223 ± 0.049	0.614GlnHis: 0.614 ± 0.024	1.965GlnIle: 1.965 ± 0.043	1.298GlnLys: 1.298 ± 0.036	4.206GlnLeu: 4.206 ± 0.084	0.798GlnMet: 0.798 ± 0.026	1.414GlnAsn: 1.414 ± 0.038	1.719GlnPro: 1.719 ± 0.04	1.683GlnGln: 1.683 ± 0.042	2.358GlnArg: 2.358 ± 0.048	1.899GlnSer: 1.899 ± 0.038	2.224GlnThr: 2.224 ± 0.053	2.42GlnVal: 2.42 ± 0.045	0.426GlnTrp: 0.426 ± 0.021	1.233GlnTyr: 1.233 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	4.366ArgAla: 4.366 ± 0.078	0.355ArgCys: 0.355 ± 0.018	3.134ArgAsp: 3.134 ± 0.06	3.589ArgGlu: 3.589 ± 0.077	2.627ArgPhe: 2.627 ± 0.058	3.685ArgGly: 3.685 ± 0.065	1.038ArgHis: 1.038 ± 0.034	3.42ArgIle: 3.42 ± 0.058	2.568ArgLys: 2.568 ± 0.06	5.531ArgLeu: 5.531 ± 0.102	1.388ArgMet: 1.388 ± 0.033	2.425ArgAsn: 2.425 ± 0.046	2.387ArgPro: 2.387 ± 0.046	2.278ArgGln: 2.278 ± 0.055	3.345ArgArg: 3.345 ± 0.07	3.141ArgSer: 3.141 ± 0.065	3.204ArgThr: 3.204 ± 0.066	3.685ArgVal: 3.685 ± 0.061	0.739ArgTrp: 0.739 ± 0.024	2.364ArgTyr: 2.364 ± 0.054	0.0ArgXaa: 0.0 ± 0.0
Ser	5.04SerAla: 5.04 ± 0.081	0.597SerCys: 0.597 ± 0.03	3.376SerAsp: 3.376 ± 0.075	2.934SerGlu: 2.934 ± 0.053	2.906SerPhe: 2.906 ± 0.055	5.29SerGly: 5.29 ± 0.106	0.904SerHis: 0.904 ± 0.03	3.51SerIle: 3.51 ± 0.055	2.003SerLys: 2.003 ± 0.055	5.914SerLeu: 5.914 ± 0.071	1.009SerMet: 1.009 ± 0.029	2.506SerAsn: 2.506 ± 0.046	2.833SerPro: 2.833 ± 0.048	1.788SerGln: 1.788 ± 0.042	2.986SerArg: 2.986 ± 0.066	3.671SerSer: 3.671 ± 0.079	3.871SerThr: 3.871 ± 0.083	4.216SerVal: 4.216 ± 0.059	0.733SerTrp: 0.733 ± 0.025	2.131SerTyr: 2.131 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	5.695ThrAla: 5.695 ± 0.108	0.652ThrCys: 0.652 ± 0.047	4.58ThrAsp: 4.58 ± 0.147	3.874ThrGlu: 3.874 ± 0.073	3.088ThrPhe: 3.088 ± 0.059	5.636ThrGly: 5.636 ± 0.138	0.959ThrHis: 0.959 ± 0.03	4.093ThrIle: 4.093 ± 0.132	2.059ThrLys: 2.059 ± 0.044	6.276ThrLeu: 6.276 ± 0.122	1.046ThrMet: 1.046 ± 0.035	2.76ThrAsn: 2.76 ± 0.068	3.279ThrPro: 3.279 ± 0.078	1.989ThrGln: 1.989 ± 0.051	2.64ThrArg: 2.64 ± 0.049	3.765ThrSer: 3.765 ± 0.096	4.586ThrThr: 4.586 ± 0.199	5.507ThrVal: 5.507 ± 0.195	0.737ThrTrp: 0.737 ± 0.027	2.693ThrTyr: 2.693 ± 0.073	0.0ThrXaa: 0.0 ± 0.0
Val	6.428ValAla: 6.428 ± 0.076	0.661ValCys: 0.661 ± 0.038	4.785ValAsp: 4.785 ± 0.085	4.373ValGlu: 4.373 ± 0.069	2.913ValPhe: 2.913 ± 0.057	5.001ValGly: 5.001 ± 0.066	1.11ValHis: 1.11 ± 0.034	3.93ValIle: 3.93 ± 0.058	2.652ValLys: 2.652 ± 0.054	6.025ValLeu: 6.025 ± 0.077	1.348ValMet: 1.348 ± 0.038	3.148ValAsn: 3.148 ± 0.071	3.077ValPro: 3.077 ± 0.069	2.141ValGln: 2.141 ± 0.036	3.751ValArg: 3.751 ± 0.061	4.425ValSer: 4.425 ± 0.067	5.347ValThr: 5.347 ± 0.217	5.088ValVal: 5.088 ± 0.115	0.77ValTrp: 0.77 ± 0.024	2.352ValTyr: 2.352 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	0.885TrpAla: 0.885 ± 0.03	0.096TrpCys: 0.096 ± 0.007	0.683TrpAsp: 0.683 ± 0.023	0.73TrpGlu: 0.73 ± 0.025	0.485TrpPhe: 0.485 ± 0.02	0.826TrpGly: 0.826 ± 0.027	0.249TrpHis: 0.249 ± 0.012	0.576TrpIle: 0.576 ± 0.022	0.546TrpLys: 0.546 ± 0.021	1.239TrpLeu: 1.239 ± 0.036	0.347TrpMet: 0.347 ± 0.018	0.549TrpAsn: 0.549 ± 0.023	0.443TrpPro: 0.443 ± 0.021	0.509TrpGln: 0.509 ± 0.021	0.731TrpArg: 0.731 ± 0.023	0.893TrpSer: 0.893 ± 0.04	0.776TrpThr: 0.776 ± 0.029	0.772TrpVal: 0.772 ± 0.024	0.218TrpTrp: 0.218 ± 0.013	0.461TrpTyr: 0.461 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.021TyrAla: 3.021 ± 0.065	0.296TyrCys: 0.296 ± 0.015	2.472TyrAsp: 2.472 ± 0.044	2.152TyrGlu: 2.152 ± 0.063	1.908TyrPhe: 1.908 ± 0.05	2.725TyrGly: 2.725 ± 0.06	0.739TyrHis: 0.739 ± 0.027	1.369TyrIle: 1.369 ± 0.033	1.125TyrLys: 1.125 ± 0.037	3.883TyrLeu: 3.883 ± 0.089	0.463TyrMet: 0.463 ± 0.022	1.577TyrAsn: 1.577 ± 0.043	1.584TyrPro: 1.584 ± 0.048	1.575TyrGln: 1.575 ± 0.035	2.607TyrArg: 2.607 ± 0.055	2.019TyrSer: 2.019 ± 0.043	2.276TyrThr: 2.276 ± 0.061	2.505TyrVal: 2.505 ± 0.048	0.505TyrTrp: 0.505 ± 0.021	1.499TyrTyr: 1.499 ± 0.047	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3730 proteins (1497538 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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