Amino acid dipepetide frequency for Loigolactobacillus backii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.447AlaAla: 9.447 ± 0.368
0.406AlaCys: 0.406 ± 0.027
5.047AlaAsp: 5.047 ± 0.104
4.28AlaGlu: 4.28 ± 0.088
3.203AlaPhe: 3.203 ± 0.074
6.252AlaGly: 6.252 ± 0.122
1.557AlaHis: 1.557 ± 0.049
6.036AlaIle: 6.036 ± 0.099
6.236AlaLys: 6.236 ± 0.134
8.236AlaLeu: 8.236 ± 0.147
2.107AlaMet: 2.107 ± 0.06
4.18AlaAsn: 4.18 ± 0.087
2.537AlaPro: 2.537 ± 0.07
4.146AlaGln: 4.146 ± 0.113
2.85AlaArg: 2.85 ± 0.071
5.297AlaSer: 5.297 ± 0.35
6.345AlaThr: 6.345 ± 0.192
6.026AlaVal: 6.026 ± 0.114
0.657AlaTrp: 0.657 ± 0.03
2.624AlaTyr: 2.624 ± 0.061
0.0AlaXaa: 0.0 ± 0.0
Cys
0.385CysAla: 0.385 ± 0.023
0.066CysCys: 0.066 ± 0.011
0.259CysAsp: 0.259 ± 0.022
0.25CysGlu: 0.25 ± 0.021
0.25CysPhe: 0.25 ± 0.016
0.477CysGly: 0.477 ± 0.029
0.171CysHis: 0.171 ± 0.017
0.261CysIle: 0.261 ± 0.018
0.165CysLys: 0.165 ± 0.017
0.597CysLeu: 0.597 ± 0.029
0.089CysMet: 0.089 ± 0.013
0.162CysAsn: 0.162 ± 0.017
0.262CysPro: 0.262 ± 0.023
0.266CysGln: 0.266 ± 0.022
0.209CysArg: 0.209 ± 0.018
0.303CysSer: 0.303 ± 0.02
0.262CysThr: 0.262 ± 0.019
0.316CysVal: 0.316 ± 0.024
0.074CysTrp: 0.074 ± 0.01
0.191CysTyr: 0.191 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
4.1AspAla: 4.1 ± 0.12
0.261AspCys: 0.261 ± 0.018
3.069AspAsp: 3.069 ± 0.084
3.334AspGlu: 3.334 ± 0.085
2.734AspPhe: 2.734 ± 0.063
3.32AspGly: 3.32 ± 0.094
1.361AspHis: 1.361 ± 0.041
3.246AspIle: 3.246 ± 0.071
3.102AspLys: 3.102 ± 0.081
5.686AspLeu: 5.686 ± 0.091
1.318AspMet: 1.318 ± 0.044
2.232AspAsn: 2.232 ± 0.065
2.335AspPro: 2.335 ± 0.108
3.076AspGln: 3.076 ± 0.074
2.158AspArg: 2.158 ± 0.06
2.787AspSer: 2.787 ± 0.075
2.779AspThr: 2.779 ± 0.072
3.767AspVal: 3.767 ± 0.079
0.696AspTrp: 0.696 ± 0.036
2.553AspTyr: 2.553 ± 0.059
0.0AspXaa: 0.0 ± 0.0
Glu
4.113GluAla: 4.113 ± 0.087
0.17GluCys: 0.17 ± 0.014
2.344GluAsp: 2.344 ± 0.071
2.597GluGlu: 2.597 ± 0.073
1.856GluPhe: 1.856 ± 0.057
2.367GluGly: 2.367 ± 0.079
1.152GluHis: 1.152 ± 0.043
3.612GluIle: 3.612 ± 0.085
3.948GluLys: 3.948 ± 0.094
5.651GluLeu: 5.651 ± 0.098
1.58GluMet: 1.58 ± 0.056
2.553GluAsn: 2.553 ± 0.057
1.654GluPro: 1.654 ± 0.054
3.207GluGln: 3.207 ± 0.08
2.54GluArg: 2.54 ± 0.075
2.299GluSer: 2.299 ± 0.066
3.236GluThr: 3.236 ± 0.076
3.483GluVal: 3.483 ± 0.082
0.385GluTrp: 0.385 ± 0.028
1.376GluTyr: 1.376 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
3.399PheAla: 3.399 ± 0.073
0.264PheCys: 0.264 ± 0.022
2.512PheAsp: 2.512 ± 0.06
1.963PheGlu: 1.963 ± 0.058
1.91PhePhe: 1.91 ± 0.066
3.197PheGly: 3.197 ± 0.076
0.839PheHis: 0.839 ± 0.039
3.053PheIle: 3.053 ± 0.087
2.578PheLys: 2.578 ± 0.065
3.842PheLeu: 3.842 ± 0.102
1.046PheMet: 1.046 ± 0.044
2.15PheAsn: 2.15 ± 0.06
1.423PhePro: 1.423 ± 0.046
1.568PheGln: 1.568 ± 0.044
1.371PheArg: 1.371 ± 0.043
2.936PheSer: 2.936 ± 0.066
2.623PheThr: 2.623 ± 0.054
2.903PheVal: 2.903 ± 0.067
0.514PheTrp: 0.514 ± 0.029
1.606PheTyr: 1.606 ± 0.056
0.0PheXaa: 0.0 ± 0.0
Gly
4.972GlyAla: 4.972 ± 0.107
0.39GlyCys: 0.39 ± 0.025
3.244GlyAsp: 3.244 ± 0.083
3.074GlyGlu: 3.074 ± 0.068
3.017GlyPhe: 3.017 ± 0.069
4.388GlyGly: 4.388 ± 0.095
1.52GlyHis: 1.52 ± 0.046
5.304GlyIle: 5.304 ± 0.101
4.219GlyLys: 4.219 ± 0.089
6.728GlyLeu: 6.728 ± 0.121
1.792GlyMet: 1.792 ± 0.051
2.786GlyAsn: 2.786 ± 0.071
1.641GlyPro: 1.641 ± 0.05
3.117GlyGln: 3.117 ± 0.088
2.616GlyArg: 2.616 ± 0.078
3.806GlySer: 3.806 ± 0.08
4.455GlyThr: 4.455 ± 0.139
4.802GlyVal: 4.802 ± 0.102
0.69GlyTrp: 0.69 ± 0.039
2.745GlyTyr: 2.745 ± 0.072
0.0GlyXaa: 0.0 ± 0.0
His
1.453HisAla: 1.453 ± 0.053
0.148HisCys: 0.148 ± 0.017
1.17HisAsp: 1.17 ± 0.043
1.128HisGlu: 1.128 ± 0.042
1.163HisPhe: 1.163 ± 0.046
1.425HisGly: 1.425 ± 0.051
0.665HisHis: 0.665 ± 0.032
1.23HisIle: 1.23 ± 0.046
0.936HisLys: 0.936 ± 0.037
2.408HisLeu: 2.408 ± 0.069
0.499HisMet: 0.499 ± 0.025
0.92HisAsn: 0.92 ± 0.038
0.979HisPro: 0.979 ± 0.039
1.152HisGln: 1.152 ± 0.044
0.913HisArg: 0.913 ± 0.039
1.022HisSer: 1.022 ± 0.039
0.995HisThr: 0.995 ± 0.036
1.433HisVal: 1.433 ± 0.044
0.261HisTrp: 0.261 ± 0.019
1.142HisTyr: 1.142 ± 0.044
0.0HisXaa: 0.0 ± 0.0
Ile
5.865IleAla: 5.865 ± 0.113
0.439IleCys: 0.439 ± 0.023
3.94IleAsp: 3.94 ± 0.078
3.35IleGlu: 3.35 ± 0.077
2.911IlePhe: 2.911 ± 0.08
5.058IleGly: 5.058 ± 0.114
1.278IleHis: 1.278 ± 0.044
4.903IleIle: 4.903 ± 0.099
4.132IleLys: 4.132 ± 0.083
6.465IleLeu: 6.465 ± 0.122
1.61IleMet: 1.61 ± 0.057
3.43IleAsn: 3.43 ± 0.071
2.689IlePro: 2.689 ± 0.059
2.793IleGln: 2.793 ± 0.068
2.55IleArg: 2.55 ± 0.066
4.275IleSer: 4.275 ± 0.085
4.443IleThr: 4.443 ± 0.11
4.915IleVal: 4.915 ± 0.098
0.632IleTrp: 0.632 ± 0.028
2.161IleTyr: 2.161 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
4.984LysAla: 4.984 ± 0.101
0.148LysCys: 0.148 ± 0.016
3.141LysAsp: 3.141 ± 0.076
3.472LysGlu: 3.472 ± 0.082
1.821LysPhe: 1.821 ± 0.057
3.088LysGly: 3.088 ± 0.066
1.246LysHis: 1.246 ± 0.045
4.293LysIle: 4.293 ± 0.08
4.842LysLys: 4.842 ± 0.102
5.97LysLeu: 5.97 ± 0.084
2.006LysMet: 2.006 ± 0.049
3.254LysAsn: 3.254 ± 0.077
2.224LysPro: 2.224 ± 0.061
4.293LysGln: 4.293 ± 0.099
3.492LysArg: 3.492 ± 0.08
3.311LysSer: 3.311 ± 0.071
4.354LysThr: 4.354 ± 0.104
4.385LysVal: 4.385 ± 0.095
0.602LysTrp: 0.602 ± 0.033
1.924LysTyr: 1.924 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
10.128LeuAla: 10.128 ± 0.163
0.577LeuCys: 0.577 ± 0.029
5.179LeuAsp: 5.179 ± 0.092
4.594LeuGlu: 4.594 ± 0.096
4.316LeuPhe: 4.316 ± 0.111
6.585LeuGly: 6.585 ± 0.116
1.839LeuHis: 1.839 ± 0.061
7.123LeuIle: 7.123 ± 0.132
6.366LeuLys: 6.366 ± 0.107
10.479LeuLeu: 10.479 ± 0.228
2.402LeuMet: 2.402 ± 0.072
5.004LeuAsn: 5.004 ± 0.095
4.524LeuPro: 4.524 ± 0.083
4.361LeuGln: 4.361 ± 0.086
4.113LeuArg: 4.113 ± 0.081
6.003LeuSer: 6.003 ± 0.11
7.776LeuThr: 7.776 ± 0.141
7.071LeuVal: 7.071 ± 0.126
0.814LeuTrp: 0.814 ± 0.038
2.86LeuTyr: 2.86 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
2.544MetAla: 2.544 ± 0.054
0.12MetCys: 0.12 ± 0.012
1.117MetAsp: 1.117 ± 0.041
0.945MetGlu: 0.945 ± 0.043
0.839MetPhe: 0.839 ± 0.035
1.567MetGly: 1.567 ± 0.052
0.453MetHis: 0.453 ± 0.029
1.68MetIle: 1.68 ± 0.055
1.652MetLys: 1.652 ± 0.046
2.392MetLeu: 2.392 ± 0.067
0.662MetMet: 0.662 ± 0.034
1.185MetAsn: 1.185 ± 0.04
1.026MetPro: 1.026 ± 0.038
1.277MetGln: 1.277 ± 0.044
1.094MetArg: 1.094 ± 0.043
1.444MetSer: 1.444 ± 0.043
2.041MetThr: 2.041 ± 0.056
1.58MetVal: 1.58 ± 0.048
0.162MetTrp: 0.162 ± 0.017
0.623MetTyr: 0.623 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.486AsnAla: 3.486 ± 0.079
0.25AsnCys: 0.25 ± 0.021
2.706AsnAsp: 2.706 ± 0.075
2.583AsnGlu: 2.583 ± 0.062
2.013AsnPhe: 2.013 ± 0.055
3.518AsnGly: 3.518 ± 0.087
1.09AsnHis: 1.09 ± 0.039
2.754AsnIle: 2.754 ± 0.061
2.55AsnLys: 2.55 ± 0.062
4.598AsnLeu: 4.598 ± 0.079
1.219AsnMet: 1.219 ± 0.042
2.348AsnAsn: 2.348 ± 0.089
2.104AsnPro: 2.104 ± 0.079
2.872AsnGln: 2.872 ± 0.079
1.994AsnArg: 1.994 ± 0.064
2.691AsnSer: 2.691 ± 0.092
2.635AsnThr: 2.635 ± 0.08
3.074AsnVal: 3.074 ± 0.065
0.615AsnTrp: 0.615 ± 0.031
2.087AsnTyr: 2.087 ± 0.077
0.0AsnXaa: 0.0 ± 0.0
Pro
3.275ProAla: 3.275 ± 0.088
0.141ProCys: 0.141 ± 0.014
2.238ProAsp: 2.238 ± 0.064
2.752ProGlu: 2.752 ± 0.072
1.645ProPhe: 1.645 ± 0.055
2.199ProGly: 2.199 ± 0.065
0.678ProHis: 0.678 ± 0.035
2.636ProIle: 2.636 ± 0.053
2.549ProLys: 2.549 ± 0.084
3.552ProLeu: 3.552 ± 0.084
0.686ProMet: 0.686 ± 0.03
1.824ProAsn: 1.824 ± 0.065
0.64ProPro: 0.64 ± 0.034
1.775ProGln: 1.775 ± 0.053
1.277ProArg: 1.277 ± 0.048
1.921ProSer: 1.921 ± 0.058
2.887ProThr: 2.887 ± 0.117
2.901ProVal: 2.901 ± 0.073
0.304ProTrp: 0.304 ± 0.018
1.306ProTyr: 1.306 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
4.784GlnAla: 4.784 ± 0.113
0.145GlnCys: 0.145 ± 0.015
2.135GlnAsp: 2.135 ± 0.058
2.411GlnGlu: 2.411 ± 0.064
1.909GlnPhe: 1.909 ± 0.047
2.401GlnGly: 2.401 ± 0.057
1.166GlnHis: 1.166 ± 0.042
3.559GlnIle: 3.559 ± 0.071
3.339GlnLys: 3.339 ± 0.082
6.157GlnLeu: 6.157 ± 0.155
1.192GlnMet: 1.192 ± 0.044
2.44GlnAsn: 2.44 ± 0.078
1.925GlnPro: 1.925 ± 0.047
3.976GlnGln: 3.976 ± 0.129
2.493GlnArg: 2.493 ± 0.066
2.575GlnSer: 2.575 ± 0.077
3.643GlnThr: 3.643 ± 0.095
3.713GlnVal: 3.713 ± 0.073
0.464GlnTrp: 0.464 ± 0.027
1.592GlnTyr: 1.592 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
2.843ArgAla: 2.843 ± 0.072
0.201ArgCys: 0.201 ± 0.018
2.155ArgAsp: 2.155 ± 0.063
2.196ArgGlu: 2.196 ± 0.061
1.904ArgPhe: 1.904 ± 0.057
2.257ArgGly: 2.257 ± 0.07
1.1ArgHis: 1.1 ± 0.039
2.655ArgIle: 2.655 ± 0.069
2.547ArgLys: 2.547 ± 0.067
4.594ArgLeu: 4.594 ± 0.093
0.98ArgMet: 0.98 ± 0.041
1.804ArgAsn: 1.804 ± 0.059
1.704ArgPro: 1.704 ± 0.05
2.837ArgGln: 2.837 ± 0.074
2.229ArgArg: 2.229 ± 0.07
2.052ArgSer: 2.052 ± 0.056
2.277ArgThr: 2.277 ± 0.063
2.649ArgVal: 2.649 ± 0.08
0.367ArgTrp: 0.367 ± 0.024
1.783ArgTyr: 1.783 ± 0.059
0.0ArgXaa: 0.0 ± 0.0
Ser
5.012SerAla: 5.012 ± 0.305
0.294SerCys: 0.294 ± 0.021
3.345SerAsp: 3.345 ± 0.088
3.016SerGlu: 3.016 ± 0.076
2.641SerPhe: 2.641 ± 0.06
4.552SerGly: 4.552 ± 0.117
1.206SerHis: 1.206 ± 0.046
3.44SerIle: 3.44 ± 0.069
3.115SerLys: 3.115 ± 0.072
5.863SerLeu: 5.863 ± 0.11
1.322SerMet: 1.322 ± 0.04
2.606SerAsn: 2.606 ± 0.092
1.861SerPro: 1.861 ± 0.049
2.652SerGln: 2.652 ± 0.072
2.312SerArg: 2.312 ± 0.06
4.063SerSer: 4.063 ± 0.313
3.743SerThr: 3.743 ± 0.16
4.071SerVal: 4.071 ± 0.08
0.577SerTrp: 0.577 ± 0.028
2.132SerTyr: 2.132 ± 0.065
0.0SerXaa: 0.0 ± 0.0
Thr
6.636ThrAla: 6.636 ± 0.239
0.298ThrCys: 0.298 ± 0.023
4.017ThrAsp: 4.017 ± 0.113
3.194ThrGlu: 3.194 ± 0.071
2.588ThrPhe: 2.588 ± 0.067
4.873ThrGly: 4.873 ± 0.12
1.191ThrHis: 1.191 ± 0.04
4.735ThrIle: 4.735 ± 0.095
4.395ThrLys: 4.395 ± 0.08
6.444ThrLeu: 6.444 ± 0.116
1.311ThrMet: 1.311 ± 0.042
3.285ThrAsn: 3.285 ± 0.086
3.097ThrPro: 3.097 ± 0.142
2.793ThrGln: 2.793 ± 0.073
2.044ThrArg: 2.044 ± 0.063
4.05ThrSer: 4.05 ± 0.176
5.379ThrThr: 5.379 ± 0.162
4.975ThrVal: 4.975 ± 0.142
0.612ThrTrp: 0.612 ± 0.033
2.314ThrTyr: 2.314 ± 0.082
0.0ThrXaa: 0.0 ± 0.0
Val
6.847ValAla: 6.847 ± 0.13
0.413ValCys: 0.413 ± 0.026
3.909ValAsp: 3.909 ± 0.1
3.127ValGlu: 3.127 ± 0.086
2.624ValPhe: 2.624 ± 0.069
4.864ValGly: 4.864 ± 0.09
1.324ValHis: 1.324 ± 0.045
4.927ValIle: 4.927 ± 0.101
4.277ValLys: 4.277 ± 0.079
6.713ValLeu: 6.713 ± 0.113
1.702ValMet: 1.702 ± 0.054
3.182ValAsn: 3.182 ± 0.067
2.791ValPro: 2.791 ± 0.064
2.717ValGln: 2.717 ± 0.067
2.556ValArg: 2.556 ± 0.066
4.471ValSer: 4.471 ± 0.087
5.69ValThr: 5.69 ± 0.126
5.251ValVal: 5.251 ± 0.097
0.558ValTrp: 0.558 ± 0.028
2.143ValTyr: 2.143 ± 0.064
0.0ValXaa: 0.0 ± 0.0
Trp
0.602TrpAla: 0.602 ± 0.032
0.068TrpCys: 0.068 ± 0.009
0.418TrpAsp: 0.418 ± 0.027
0.321TrpGlu: 0.321 ± 0.026
0.51TrpPhe: 0.51 ± 0.027
0.593TrpGly: 0.593 ± 0.031
0.27TrpHis: 0.27 ± 0.018
0.569TrpIle: 0.569 ± 0.026
0.365TrpLys: 0.365 ± 0.024
1.493TrpLeu: 1.493 ± 0.058
0.176TrpMet: 0.176 ± 0.015
0.382TrpAsn: 0.382 ± 0.023
0.349TrpPro: 0.349 ± 0.023
0.756TrpGln: 0.756 ± 0.036
0.54TrpArg: 0.54 ± 0.025
0.51TrpSer: 0.51 ± 0.027
0.481TrpThr: 0.481 ± 0.03
0.593TrpVal: 0.593 ± 0.033
0.149TrpTrp: 0.149 ± 0.016
0.404TrpTyr: 0.404 ± 0.036
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.592TyrAla: 2.592 ± 0.066
0.216TyrCys: 0.216 ± 0.02
1.966TyrAsp: 1.966 ± 0.069
1.535TyrGlu: 1.535 ± 0.053
1.845TyrPhe: 1.845 ± 0.058
2.326TyrGly: 2.326 ± 0.059
0.933TyrHis: 0.933 ± 0.037
1.826TyrIle: 1.826 ± 0.048
1.538TyrLys: 1.538 ± 0.048
4.248TyrLeu: 4.248 ± 0.074
0.707TyrMet: 0.707 ± 0.032
1.461TyrAsn: 1.461 ± 0.053
1.421TyrPro: 1.421 ± 0.048
2.384TyrGln: 2.384 ± 0.067
1.785TyrArg: 1.785 ± 0.053
2.003TyrSer: 2.003 ± 0.065
2.185TyrThr: 2.185 ± 0.1
2.181TyrVal: 2.181 ± 0.057
0.417TyrTrp: 0.417 ± 0.023
1.454TyrTyr: 1.454 ± 0.056
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2375 proteins (717253 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski