Roundleaf bat hepatitis B virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Orthohepadnavirus

Average proteome isoelectric point is 9.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3M9X7|U3M9X7_9HEPA DNA-directed DNA polymerase OS=Roundleaf bat hepatitis B virus OX=1508710 PE=3 SV=1
MM1 pKa = 7.41GNISSEE7 pKa = 4.09LLGPLVGFQVVFFLWTKK24 pKa = 10.23ILTIAQSLDD33 pKa = 3.44SWWTSLSFPGGIPEE47 pKa = 4.38CFGLNLQSQTCKK59 pKa = 10.4HH60 pKa = 6.5SPTSCPPTCAGFRR73 pKa = 11.84WMCLRR78 pKa = 11.84RR79 pKa = 11.84FIIYY83 pKa = 10.44LLVLLLCLIFLLVLLDD99 pKa = 3.51WRR101 pKa = 11.84GLLPVCPLNPNNQTTMRR118 pKa = 11.84CTSCTISADD127 pKa = 3.29ALTSFPYY134 pKa = 10.14CCCSKK139 pKa = 10.64PSGGNCTCWPIPSSWAFAKK158 pKa = 9.66FLWEE162 pKa = 3.86LASLRR167 pKa = 11.84FSWLNSLVPWLQWFAGLSPTAWLLAIWMMWYY198 pKa = 9.3WGPGLLNTLSPFMPLFVLFFLLWGYY223 pKa = 10.07II224 pKa = 3.63

Molecular weight: 25.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3M9Y2|U3M9Y2_9HEPA Protein X OS=Roundleaf bat hepatitis B virus OX=1508710 GN=X PE=3 SV=1
MM1 pKa = 7.5HH2 pKa = 7.46PFSRR6 pKa = 11.84LFRR9 pKa = 11.84SIQSLGEE16 pKa = 4.2EE17 pKa = 4.24EE18 pKa = 4.34VHH20 pKa = 6.94ALLGPPEE27 pKa = 4.38GALPLLADD35 pKa = 4.09ADD37 pKa = 4.19LNRR40 pKa = 11.84RR41 pKa = 11.84VAADD45 pKa = 4.73LSLQLPPTASIWVHH59 pKa = 5.29KK60 pKa = 10.81AGGLTGLYY68 pKa = 9.82SDD70 pKa = 4.79KK71 pKa = 10.65PKK73 pKa = 10.89SFNPHH78 pKa = 4.36WKK80 pKa = 8.34TPEE83 pKa = 3.75FPQLHH88 pKa = 6.52LRR90 pKa = 11.84PDD92 pKa = 4.25LIEE95 pKa = 4.28YY96 pKa = 7.61LTHH99 pKa = 7.28RR100 pKa = 11.84LGPLTSAEE108 pKa = 3.71KK109 pKa = 10.45RR110 pKa = 11.84RR111 pKa = 11.84FRR113 pKa = 11.84LCIPARR119 pKa = 11.84FFPNKK124 pKa = 8.65TKK126 pKa = 10.78YY127 pKa = 9.41FPLYY131 pKa = 10.4KK132 pKa = 10.13GIKK135 pKa = 9.15PYY137 pKa = 11.16YY138 pKa = 8.5PDD140 pKa = 3.58NLLTHH145 pKa = 7.0FFLTSHH151 pKa = 6.39YY152 pKa = 10.92LRR154 pKa = 11.84TLWEE158 pKa = 4.22AGVTYY163 pKa = 10.56LRR165 pKa = 11.84EE166 pKa = 4.32TQSSASFNGSPYY178 pKa = 9.86PWEE181 pKa = 4.18QKK183 pKa = 7.31QHH185 pKa = 4.1NHH187 pKa = 3.88GTEE190 pKa = 4.38RR191 pKa = 11.84INSKK195 pKa = 9.96PSRR198 pKa = 11.84VLPGTRR204 pKa = 11.84STTRR208 pKa = 11.84LRR210 pKa = 11.84KK211 pKa = 9.77AGNGPCTRR219 pKa = 11.84PLHH222 pKa = 6.58LLGQSPLQRR231 pKa = 11.84SRR233 pKa = 11.84QRR235 pKa = 11.84EE236 pKa = 3.77ASSLGKK242 pKa = 10.42GRR244 pKa = 11.84SSPRR248 pKa = 11.84LGSRR252 pKa = 11.84THH254 pKa = 5.99TSTRR258 pKa = 11.84RR259 pKa = 11.84PFGVSRR265 pKa = 11.84DD266 pKa = 3.81LPGGTNHH273 pKa = 6.36FPPRR277 pKa = 11.84WLDD280 pKa = 3.29HH281 pKa = 6.69KK282 pKa = 10.97NSNRR286 pKa = 11.84QTSHH290 pKa = 5.44HH291 pKa = 6.96HH292 pKa = 5.27STSTYY297 pKa = 6.31QTKK300 pKa = 8.53QAKK303 pKa = 9.67GGAKK307 pKa = 9.93GRR309 pKa = 11.84ILPNSYY315 pKa = 7.78TTATSYY321 pKa = 8.51RR322 pKa = 11.84THH324 pKa = 6.5QSEE327 pKa = 4.33AFSDD331 pKa = 3.95TSHH334 pKa = 6.39NTTQSEE340 pKa = 4.26QGEE343 pKa = 4.67DD344 pKa = 3.03THH346 pKa = 8.78SPYY349 pKa = 10.92SSGAGHH355 pKa = 6.45SSSPEE360 pKa = 3.44HH361 pKa = 6.67DD362 pKa = 3.6QPDD365 pKa = 3.61VEE367 pKa = 4.6GAIRR371 pKa = 11.84PSPLCAWSYY380 pKa = 10.75LRR382 pKa = 11.84SHH384 pKa = 7.41PACGPHH390 pKa = 7.07CLKK393 pKa = 10.66QCTLLLDD400 pKa = 3.75DD401 pKa = 5.12WGPCSRR407 pKa = 11.84HH408 pKa = 5.54GEE410 pKa = 4.09HH411 pKa = 7.92LIRR414 pKa = 11.84TPRR417 pKa = 11.84TPRR420 pKa = 11.84RR421 pKa = 11.84ISGGVFLVDD430 pKa = 3.82KK431 pKa = 10.6NPNNSAEE438 pKa = 4.07SRR440 pKa = 11.84LVVDD444 pKa = 5.52FSQFSRR450 pKa = 11.84GDD452 pKa = 3.37SRR454 pKa = 11.84VFWPKK459 pKa = 9.96FAVPNLQALTNLLSTNLCWLSLDD482 pKa = 3.51VSAAFYY488 pKa = 9.93HH489 pKa = 6.73IPLSPAAMPHH499 pKa = 6.39LLVGSPGLEE508 pKa = 3.52GLAASVSPQPKK519 pKa = 9.68QPDD522 pKa = 3.32NHH524 pKa = 6.51EE525 pKa = 3.82MHH527 pKa = 6.99ILHH530 pKa = 6.34NLCGRR535 pKa = 11.84ANLLSILLLFKK546 pKa = 10.33TFGRR550 pKa = 11.84EE551 pKa = 3.73LHH553 pKa = 6.39LLAHH557 pKa = 5.99SFIMGFRR564 pKa = 11.84KK565 pKa = 9.59IPMGVGLSPFLLAQFTSALASVVRR589 pKa = 11.84RR590 pKa = 11.84TFAHH594 pKa = 6.26CVAFSYY600 pKa = 10.57MDD602 pKa = 3.85DD603 pKa = 3.59VVLGARR609 pKa = 11.84TPQHH613 pKa = 6.43LEE615 pKa = 3.56SVYY618 pKa = 11.29ASVCSLFSSLGIHH631 pKa = 6.95LNPSKK636 pKa = 9.86TKK638 pKa = 8.1WWGKK642 pKa = 8.9HH643 pKa = 3.95LHH645 pKa = 5.99FMGLVLSPAGALPQTKK661 pKa = 8.97HH662 pKa = 5.07QSRR665 pKa = 11.84ALSCLKK671 pKa = 10.39RR672 pKa = 11.84LPTYY676 pKa = 10.76VVLDD680 pKa = 3.74WKK682 pKa = 10.74LLQRR686 pKa = 11.84LTGLLGFLAPFTACGYY702 pKa = 7.21PALMPLYY709 pKa = 10.49AAIQARR715 pKa = 11.84RR716 pKa = 11.84GFEE719 pKa = 3.51FSPAYY724 pKa = 9.74KK725 pKa = 10.61GFLLQLYY732 pKa = 9.76SHH734 pKa = 7.73LLPVARR740 pKa = 11.84QRR742 pKa = 11.84RR743 pKa = 11.84AVCQVFADD751 pKa = 4.09ATPTGWGLVNHH762 pKa = 5.99QLAAYY767 pKa = 8.71KK768 pKa = 10.24AGRR771 pKa = 11.84FPRR774 pKa = 11.84PLPIHH779 pKa = 5.99CAEE782 pKa = 5.51LIAACLARR790 pKa = 11.84RR791 pKa = 11.84WSGARR796 pKa = 11.84VLGVDD801 pKa = 3.36NTIVCSGKK809 pKa = 8.55FTHH812 pKa = 6.96FPWLLGCTANWMLRR826 pKa = 11.84GTSFCYY832 pKa = 10.54VPSEE836 pKa = 4.58LNPADD841 pKa = 3.82APSRR845 pKa = 11.84GLLGILLAPPPLLFRR860 pKa = 11.84PSTGRR865 pKa = 11.84TSLFAVSPPAPFHH878 pKa = 6.61RR879 pKa = 11.84PDD881 pKa = 3.31RR882 pKa = 11.84VSFASPVRR890 pKa = 11.84TFKK893 pKa = 10.45DD894 pKa = 2.97TWRR897 pKa = 11.84PPP899 pKa = 3.11

Molecular weight: 99.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1481

141

899

370.3

41.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.022 ± 0.854

3.038 ± 0.942

2.836 ± 0.411

3.174 ± 0.567

5.267 ± 0.52

6.482 ± 0.492

3.781 ± 1.066

2.903 ± 0.703

2.903 ± 0.648

13.369 ± 1.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.485 ± 0.437

2.903 ± 0.186

8.778 ± 0.316

3.579 ± 0.328

7.765 ± 1.177

8.845 ± 0.841

6.077 ± 0.364

4.456 ± 0.305

2.903 ± 1.134

2.431 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski