Armatimonadetes bacterium Uphvl-Ar2
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2524 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y0H9W7|A0A1Y0H9W7_9BACT Type-5 uracil-DNA glycosylase OS=Armatimonadetes bacterium Uphvl-Ar2 OX=2004468 GN=CCB81_11840 PE=3 SV=1
MM1 pKa = 7.73 SITLTPRR8 pKa = 11.84 ASQEE12 pKa = 3.88 LRR14 pKa = 11.84 DD15 pKa = 4.15 LMTGQEE21 pKa = 4.42 KK22 pKa = 10.56 PNAHH26 pKa = 5.94 LRR28 pKa = 11.84 VWVAGGGCSGLSYY41 pKa = 11.19 GMALDD46 pKa = 4.15 EE47 pKa = 4.96 NEE49 pKa = 4.77 PDD51 pKa = 3.71 ADD53 pKa = 4.0 DD54 pKa = 5.86 QIFEE58 pKa = 4.25 CDD60 pKa = 3.36 GVKK63 pKa = 10.44 VVVDD67 pKa = 4.23 AMSLQYY73 pKa = 10.26 MGGSSVDD80 pKa = 3.66 YY81 pKa = 10.66 VDD83 pKa = 6.0 DD84 pKa = 3.73 MMGGGFKK91 pKa = 9.99 IEE93 pKa = 4.5 NPNATKK99 pKa = 10.8 GCGCGSSFSTEE110 pKa = 4.4 DD111 pKa = 3.53 GPQGGGGCGSCGCASS126 pKa = 3.29
Molecular weight: 12.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.844
IPC2_protein 4.037
IPC_protein 3.973
Toseland 3.77
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.126
Wikipedia 3.897
Rodwell 3.808
Grimsley 3.681
Solomon 3.948
Lehninger 3.91
Nozaki 4.088
DTASelect 4.304
Thurlkill 3.834
EMBOSS 3.91
Sillero 4.101
Patrickios 0.655
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 3.971
Protein with the highest isoelectric point:
>tr|A0A1Y0H5M7|A0A1Y0H5M7_9BACT Uncharacterized protein OS=Armatimonadetes bacterium Uphvl-Ar2 OX=2004468 GN=CCB81_00690 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.4 RR12 pKa = 11.84 SKK14 pKa = 8.5 THH16 pKa = 5.45 GFRR19 pKa = 11.84 IRR21 pKa = 11.84 MRR23 pKa = 11.84 TQDD26 pKa = 3.29 GQNVLRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LGAA44 pKa = 3.96
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.44
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.112
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2524
0
2524
792677
34
2299
314.1
34.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.773 ± 0.066
0.83 ± 0.017
5.395 ± 0.03
6.252 ± 0.05
3.61 ± 0.031
8.572 ± 0.047
2.003 ± 0.026
4.649 ± 0.031
3.271 ± 0.037
9.526 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.644 ± 0.024
3.125 ± 0.032
5.477 ± 0.038
3.785 ± 0.028
7.001 ± 0.044
5.433 ± 0.032
5.809 ± 0.04
7.702 ± 0.038
1.641 ± 0.023
2.5 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here