Amino acid dipepetide frequency for Amycolatopsis saalfeldensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.396AlaAla: 21.396 ± 0.143	0.98AlaCys: 0.98 ± 0.019	8.319AlaAsp: 8.319 ± 0.067	9.085AlaGlu: 9.085 ± 0.074	3.634AlaPhe: 3.634 ± 0.04	14.392AlaGly: 14.392 ± 0.084	2.626AlaHis: 2.626 ± 0.031	4.082AlaIle: 4.082 ± 0.039	3.294AlaLys: 3.294 ± 0.041	13.959AlaLeu: 13.959 ± 0.09	2.477AlaMet: 2.477 ± 0.03	2.346AlaAsn: 2.346 ± 0.032	6.611AlaPro: 6.611 ± 0.062	3.861AlaGln: 3.861 ± 0.04	9.5AlaArg: 9.5 ± 0.068	6.211AlaSer: 6.211 ± 0.052	7.265AlaThr: 7.265 ± 0.059	12.104AlaVal: 12.104 ± 0.081	1.926AlaTrp: 1.926 ± 0.027	2.438AlaTyr: 2.438 ± 0.025	0.0AlaXaa: 0.0 ± 0.0
Cys	0.996CysAla: 0.996 ± 0.021	0.096CysCys: 0.096 ± 0.007	0.405CysAsp: 0.405 ± 0.011	0.353CysGlu: 0.353 ± 0.01	0.212CysPhe: 0.212 ± 0.009	0.859CysGly: 0.859 ± 0.017	0.187CysHis: 0.187 ± 0.007	0.15CysIle: 0.15 ± 0.007	0.099CysLys: 0.099 ± 0.006	0.699CysLeu: 0.699 ± 0.015	0.102CysMet: 0.102 ± 0.006	0.13CysAsn: 0.13 ± 0.007	0.466CysPro: 0.466 ± 0.014	0.178CysGln: 0.178 ± 0.008	0.527CysArg: 0.527 ± 0.014	0.469CysSer: 0.469 ± 0.013	0.477CysThr: 0.477 ± 0.015	0.582CysVal: 0.582 ± 0.016	0.123CysTrp: 0.123 ± 0.006	0.154CysTyr: 0.154 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	7.393AspAla: 7.393 ± 0.07	0.381AspCys: 0.381 ± 0.014	3.254AspAsp: 3.254 ± 0.041	3.838AspGlu: 3.838 ± 0.042	1.636AspPhe: 1.636 ± 0.028	6.108AspGly: 6.108 ± 0.055	1.349AspHis: 1.349 ± 0.023	1.677AspIle: 1.677 ± 0.028	1.048AspLys: 1.048 ± 0.022	6.374AspLeu: 6.374 ± 0.047	0.628AspMet: 0.628 ± 0.016	1.077AspAsn: 1.077 ± 0.024	4.365AspPro: 4.365 ± 0.047	1.799AspGln: 1.799 ± 0.028	4.621AspArg: 4.621 ± 0.04	2.655AspSer: 2.655 ± 0.029	2.901AspThr: 2.901 ± 0.033	5.114AspVal: 5.114 ± 0.047	0.954AspTrp: 0.954 ± 0.017	1.251AspTyr: 1.251 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	6.705GluAla: 6.705 ± 0.064	0.32GluCys: 0.32 ± 0.009	2.692GluAsp: 2.692 ± 0.033	2.723GluGlu: 2.723 ± 0.038	1.733GluPhe: 1.733 ± 0.027	3.619GluGly: 3.619 ± 0.038	1.578GluHis: 1.578 ± 0.023	2.253GluIle: 2.253 ± 0.026	1.267GluLys: 1.267 ± 0.025	7.138GluLeu: 7.138 ± 0.062	0.836GluMet: 0.836 ± 0.016	1.059GluAsn: 1.059 ± 0.021	3.504GluPro: 3.504 ± 0.045	2.254GluGln: 2.254 ± 0.029	4.875GluArg: 4.875 ± 0.05	2.527GluSer: 2.527 ± 0.028	2.869GluThr: 2.869 ± 0.033	4.636GluVal: 4.636 ± 0.053	0.751GluTrp: 0.751 ± 0.016	1.032GluTyr: 1.032 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	4.166PheAla: 4.166 ± 0.042	0.263PheCys: 0.263 ± 0.01	2.216PheAsp: 2.216 ± 0.028	1.456PheGlu: 1.456 ± 0.023	0.95PhePhe: 0.95 ± 0.021	3.457PheGly: 3.457 ± 0.04	0.635PheHis: 0.635 ± 0.015	0.71PheIle: 0.71 ± 0.017	0.457PheLys: 0.457 ± 0.015	2.716PheLeu: 2.716 ± 0.035	0.342PheMet: 0.342 ± 0.012	0.501PheAsn: 0.501 ± 0.012	1.473PhePro: 1.473 ± 0.024	0.682PheGln: 0.682 ± 0.016	1.868PheArg: 1.868 ± 0.025	1.671PheSer: 1.671 ± 0.025	2.177PheThr: 2.177 ± 0.027	2.427PheVal: 2.427 ± 0.03	0.43PheTrp: 0.43 ± 0.013	0.596PheTyr: 0.596 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	10.974GlyAla: 10.974 ± 0.073	0.795GlyCys: 0.795 ± 0.019	5.077GlyAsp: 5.077 ± 0.045	5.157GlyGlu: 5.157 ± 0.041	3.236GlyPhe: 3.236 ± 0.033	8.925GlyGly: 8.925 ± 0.091	2.279GlyHis: 2.279 ± 0.03	3.658GlyIle: 3.658 ± 0.034	2.562GlyLys: 2.562 ± 0.031	10.0GlyLeu: 10.0 ± 0.07	1.933GlyMet: 1.933 ± 0.026	1.924GlyAsn: 1.924 ± 0.034	5.076GlyPro: 5.076 ± 0.054	3.054GlyGln: 3.054 ± 0.046	7.208GlyArg: 7.208 ± 0.052	5.519GlySer: 5.519 ± 0.057	6.006GlyThr: 6.006 ± 0.051	7.921GlyVal: 7.921 ± 0.059	1.875GlyTrp: 1.875 ± 0.03	2.432GlyTyr: 2.432 ± 0.032	0.0GlyXaa: 0.0 ± 0.0
His	2.513HisAla: 2.513 ± 0.031	0.201HisCys: 0.201 ± 0.008	1.322HisAsp: 1.322 ± 0.022	1.2HisGlu: 1.2 ± 0.022	0.659HisPhe: 0.659 ± 0.017	2.324HisGly: 2.324 ± 0.03	0.629HisHis: 0.629 ± 0.018	0.583HisIle: 0.583 ± 0.013	0.293HisLys: 0.293 ± 0.01	2.304HisLeu: 2.304 ± 0.035	0.267HisMet: 0.267 ± 0.009	0.404HisAsn: 0.404 ± 0.011	1.678HisPro: 1.678 ± 0.025	0.698HisGln: 0.698 ± 0.016	1.922HisArg: 1.922 ± 0.026	1.069HisSer: 1.069 ± 0.019	1.172HisThr: 1.172 ± 0.018	1.739HisVal: 1.739 ± 0.023	0.367HisTrp: 0.367 ± 0.011	0.502HisTyr: 0.502 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	5.109IleAla: 5.109 ± 0.046	0.25IleCys: 0.25 ± 0.01	2.213IleAsp: 2.213 ± 0.028	2.015IleGlu: 2.015 ± 0.029	0.731IlePhe: 0.731 ± 0.016	3.852IleGly: 3.852 ± 0.042	0.575IleHis: 0.575 ± 0.013	0.876IleIle: 0.876 ± 0.02	0.683IleLys: 0.683 ± 0.013	2.473IleLeu: 2.473 ± 0.033	0.441IleMet: 0.441 ± 0.013	0.698IleAsn: 0.698 ± 0.017	1.823IlePro: 1.823 ± 0.027	0.714IleGln: 0.714 ± 0.017	2.253IleArg: 2.253 ± 0.027	1.868IleSer: 1.868 ± 0.026	2.387IleThr: 2.387 ± 0.031	3.008IleVal: 3.008 ± 0.036	0.361IleTrp: 0.361 ± 0.013	0.551IleTyr: 0.551 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	2.788LysAla: 2.788 ± 0.039	0.099LysCys: 0.099 ± 0.005	1.126LysAsp: 1.126 ± 0.022	0.933LysGlu: 0.933 ± 0.021	0.568LysPhe: 0.568 ± 0.015	1.473LysGly: 1.473 ± 0.026	0.464LysHis: 0.464 ± 0.013	0.95LysIle: 0.95 ± 0.018	0.653LysLys: 0.653 ± 0.018	2.341LysLeu: 2.341 ± 0.032	0.395LysMet: 0.395 ± 0.012	0.487LysAsn: 0.487 ± 0.016	1.573LysPro: 1.573 ± 0.03	0.768LysGln: 0.768 ± 0.017	1.443LysArg: 1.443 ± 0.021	1.126LysSer: 1.126 ± 0.022	1.367LysThr: 1.367 ± 0.025	1.86LysVal: 1.86 ± 0.029	0.279LysTrp: 0.279 ± 0.009	0.453LysTyr: 0.453 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	16.165LeuAla: 16.165 ± 0.095	0.756LeuCys: 0.756 ± 0.016	6.877LeuAsp: 6.877 ± 0.058	4.682LeuGlu: 4.682 ± 0.055	2.78LeuPhe: 2.78 ± 0.038	9.932LeuGly: 9.932 ± 0.069	2.163LeuHis: 2.163 ± 0.03	3.377LeuIle: 3.377 ± 0.04	1.906LeuLys: 1.906 ± 0.032	11.014LeuLeu: 11.014 ± 0.096	1.462LeuMet: 1.462 ± 0.025	1.811LeuAsn: 1.811 ± 0.028	6.492LeuPro: 6.492 ± 0.055	2.194LeuGln: 2.194 ± 0.03	8.516LeuArg: 8.516 ± 0.065	5.708LeuSer: 5.708 ± 0.051	6.713LeuThr: 6.713 ± 0.048	9.565LeuVal: 9.565 ± 0.069	1.285LeuTrp: 1.285 ± 0.025	1.69LeuTyr: 1.69 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	2.084MetAla: 2.084 ± 0.026	0.104MetCys: 0.104 ± 0.006	0.795MetAsp: 0.795 ± 0.018	0.547MetGlu: 0.547 ± 0.013	0.458MetPhe: 0.458 ± 0.013	1.172MetGly: 1.172 ± 0.02	0.315MetHis: 0.315 ± 0.01	0.669MetIle: 0.669 ± 0.016	0.389MetLys: 0.389 ± 0.012	1.681MetLeu: 1.681 ± 0.025	0.259MetMet: 0.259 ± 0.009	0.402MetAsn: 0.402 ± 0.012	1.105MetPro: 1.105 ± 0.021	0.411MetGln: 0.411 ± 0.013	1.281MetArg: 1.281 ± 0.019	1.246MetSer: 1.246 ± 0.019	1.556MetThr: 1.556 ± 0.021	1.253MetVal: 1.253 ± 0.02	0.191MetTrp: 0.191 ± 0.009	0.266MetTyr: 0.266 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	2.34AsnAla: 2.34 ± 0.029	0.142AsnCys: 0.142 ± 0.008	0.967AsnAsp: 0.967 ± 0.021	0.852AsnGlu: 0.852 ± 0.016	0.533AsnPhe: 0.533 ± 0.015	2.118AsnGly: 2.118 ± 0.037	0.411AsnHis: 0.411 ± 0.012	0.612AsnIle: 0.612 ± 0.015	0.362AsnLys: 0.362 ± 0.011	1.895AsnLeu: 1.895 ± 0.03	0.273AsnMet: 0.273 ± 0.01	0.469AsnAsn: 0.469 ± 0.015	1.547AsnPro: 1.547 ± 0.027	0.613AsnGln: 0.613 ± 0.016	1.308AsnArg: 1.308 ± 0.021	1.043AsnSer: 1.043 ± 0.023	1.156AsnThr: 1.156 ± 0.023	1.51AsnVal: 1.51 ± 0.029	0.304AsnTrp: 0.304 ± 0.01	0.443AsnTyr: 0.443 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.091ProAla: 9.091 ± 0.068	0.32ProCys: 0.32 ± 0.011	4.384ProAsp: 4.384 ± 0.037	4.081ProGlu: 4.081 ± 0.043	1.557ProPhe: 1.557 ± 0.022	6.893ProGly: 6.893 ± 0.073	1.193ProHis: 1.193 ± 0.025	1.621ProIle: 1.621 ± 0.027	1.254ProLys: 1.254 ± 0.024	5.173ProLeu: 5.173 ± 0.046	1.011ProMet: 1.011 ± 0.02	1.05ProAsn: 1.05 ± 0.021	3.603ProPro: 3.603 ± 0.061	1.676ProGln: 1.676 ± 0.03	3.843ProArg: 3.843 ± 0.044	3.478ProSer: 3.478 ± 0.039	2.936ProThr: 2.936 ± 0.041	5.43ProVal: 5.43 ± 0.052	0.903ProTrp: 0.903 ± 0.017	1.144ProTyr: 1.144 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	3.905GlnAla: 3.905 ± 0.043	0.178GlnCys: 0.178 ± 0.008	1.343GlnAsp: 1.343 ± 0.023	1.245GlnGlu: 1.245 ± 0.022	0.833GlnPhe: 0.833 ± 0.018	2.153GlnGly: 2.153 ± 0.034	0.673GlnHis: 0.673 ± 0.015	1.067GlnIle: 1.067 ± 0.021	0.559GlnLys: 0.559 ± 0.015	3.471GlnLeu: 3.471 ± 0.034	0.423GlnMet: 0.423 ± 0.012	0.606GlnAsn: 0.606 ± 0.017	1.937GlnPro: 1.937 ± 0.043	1.418GlnGln: 1.418 ± 0.035	2.475GlnArg: 2.475 ± 0.037	1.396GlnSer: 1.396 ± 0.027	1.412GlnThr: 1.412 ± 0.025	2.579GlnVal: 2.579 ± 0.034	0.512GlnTrp: 0.512 ± 0.013	0.61GlnTyr: 0.61 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	9.464ArgAla: 9.464 ± 0.062	0.567ArgCys: 0.567 ± 0.016	3.938ArgAsp: 3.938 ± 0.043	4.467ArgGlu: 4.467 ± 0.047	2.436ArgPhe: 2.436 ± 0.031	5.587ArgGly: 5.587 ± 0.05	1.905ArgHis: 1.905 ± 0.032	2.958ArgIle: 2.958 ± 0.032	1.732ArgLys: 1.732 ± 0.026	8.462ArgLeu: 8.462 ± 0.061	1.62ArgMet: 1.62 ± 0.025	1.354ArgAsn: 1.354 ± 0.02	4.742ArgPro: 4.742 ± 0.05	2.28ArgGln: 2.28 ± 0.031	7.334ArgArg: 7.334 ± 0.071	3.874ArgSer: 3.874 ± 0.038	4.595ArgThr: 4.595 ± 0.045	5.984ArgVal: 5.984 ± 0.054	1.401ArgTrp: 1.401 ± 0.027	1.765ArgTyr: 1.765 ± 0.027	0.0ArgXaa: 0.0 ± 0.0
Ser	7.203SerAla: 7.203 ± 0.054	0.422SerCys: 0.422 ± 0.014	2.748SerAsp: 2.748 ± 0.034	2.316SerGlu: 2.316 ± 0.025	1.651SerPhe: 1.651 ± 0.027	6.28SerGly: 6.28 ± 0.061	0.992SerHis: 0.992 ± 0.021	1.702SerIle: 1.702 ± 0.027	1.034SerLys: 1.034 ± 0.018	5.101SerLeu: 5.101 ± 0.049	1.132SerMet: 1.132 ± 0.021	0.948SerAsn: 0.948 ± 0.021	3.428SerPro: 3.428 ± 0.039	1.299SerGln: 1.299 ± 0.026	3.725SerArg: 3.725 ± 0.04	3.241SerSer: 3.241 ± 0.041	3.381SerThr: 3.381 ± 0.038	4.466SerVal: 4.466 ± 0.04	1.024SerTrp: 1.024 ± 0.019	1.21SerTyr: 1.21 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	8.61ThrAla: 8.61 ± 0.068	0.408ThrCys: 0.408 ± 0.012	3.31ThrAsp: 3.31 ± 0.038	3.15ThrGlu: 3.15 ± 0.038	1.721ThrPhe: 1.721 ± 0.026	6.706ThrGly: 6.706 ± 0.06	1.074ThrHis: 1.074 ± 0.019	1.961ThrIle: 1.961 ± 0.03	1.235ThrLys: 1.235 ± 0.025	5.645ThrLeu: 5.645 ± 0.042	0.941ThrMet: 0.941 ± 0.016	1.052ThrAsn: 1.052 ± 0.021	3.924ThrPro: 3.924 ± 0.04	1.454ThrGln: 1.454 ± 0.024	3.855ThrArg: 3.855 ± 0.035	3.37ThrSer: 3.37 ± 0.039	3.979ThrThr: 3.979 ± 0.043	6.072ThrVal: 6.072 ± 0.052	0.883ThrTrp: 0.883 ± 0.019	1.171ThrTyr: 1.171 ± 0.022	0.0ThrXaa: 0.0 ± 0.0
Val	11.5ValAla: 11.5 ± 0.073	0.655ValCys: 0.655 ± 0.016	5.345ValAsp: 5.345 ± 0.044	4.657ValGlu: 4.657 ± 0.052	2.625ValPhe: 2.625 ± 0.032	6.705ValGly: 6.705 ± 0.05	1.929ValHis: 1.929 ± 0.03	2.983ValIle: 2.983 ± 0.034	1.723ValLys: 1.723 ± 0.028	10.377ValLeu: 10.377 ± 0.083	1.185ValMet: 1.185 ± 0.025	1.77ValAsn: 1.77 ± 0.027	5.249ValPro: 5.249 ± 0.042	2.141ValGln: 2.141 ± 0.028	6.758ValArg: 6.758 ± 0.06	4.736ValSer: 4.736 ± 0.038	5.903ValThr: 5.903 ± 0.053	8.644ValVal: 8.644 ± 0.071	1.079ValTrp: 1.079 ± 0.02	1.505ValTyr: 1.505 ± 0.024	0.0ValXaa: 0.0 ± 0.0
Trp	1.665TrpAla: 1.665 ± 0.027	0.15TrpCys: 0.15 ± 0.007	0.79TrpAsp: 0.79 ± 0.018	0.631TrpGlu: 0.631 ± 0.017	0.533TrpPhe: 0.533 ± 0.014	1.055TrpGly: 1.055 ± 0.021	0.401TrpHis: 0.401 ± 0.013	0.557TrpIle: 0.557 ± 0.014	0.304TrpLys: 0.304 ± 0.011	1.997TrpLeu: 1.997 ± 0.028	0.27TrpMet: 0.27 ± 0.009	0.387TrpAsn: 0.387 ± 0.011	0.883TrpPro: 0.883 ± 0.019	0.631TrpGln: 0.631 ± 0.015	1.314TrpArg: 1.314 ± 0.022	0.999TrpSer: 0.999 ± 0.019	1.054TrpThr: 1.054 ± 0.021	1.049TrpVal: 1.049 ± 0.02	0.376TrpTrp: 0.376 ± 0.013	0.326TrpTyr: 0.326 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.44TyrAla: 2.44 ± 0.028	0.176TyrCys: 0.176 ± 0.008	1.3TyrAsp: 1.3 ± 0.027	0.986TyrGlu: 0.986 ± 0.022	0.706TyrPhe: 0.706 ± 0.015	2.003TyrGly: 2.003 ± 0.029	0.465TyrHis: 0.465 ± 0.012	0.437TyrIle: 0.437 ± 0.013	0.29TyrLys: 0.29 ± 0.012	2.268TyrLeu: 2.268 ± 0.033	0.188TyrMet: 0.188 ± 0.008	0.423TyrAsn: 0.423 ± 0.012	1.162TyrPro: 1.162 ± 0.018	0.67TyrGln: 0.67 ± 0.015	1.838TyrArg: 1.838 ± 0.025	1.094TyrSer: 1.094 ± 0.021	1.217TyrThr: 1.217 ± 0.023	1.529TyrVal: 1.529 ± 0.028	0.345TyrTrp: 0.345 ± 0.011	0.485TyrTyr: 0.485 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9167 proteins (2936693 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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