Beihai barnacle virus 7
Average proteome isoelectric point is 7.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KMQ3|A0A1L3KMQ3_9VIRU Putative glycoprotein OS=Beihai barnacle virus 7 OX=1922365 PE=4 SV=1
MM1 pKa = 6.37 TTIKK5 pKa = 10.71 AFLSGQVEE13 pKa = 4.21 GLPASEE19 pKa = 4.24 QEE21 pKa = 4.17 PSNVEE26 pKa = 3.99 CKK28 pKa = 10.41 DD29 pKa = 3.41 ALTDD33 pKa = 3.9 RR34 pKa = 11.84 LKK36 pKa = 10.81 KK37 pKa = 10.12 VYY39 pKa = 10.33 NYY41 pKa = 10.53 RR42 pKa = 11.84 DD43 pKa = 3.88 DD44 pKa = 3.45 SHH46 pKa = 6.94 KK47 pKa = 11.27 ALLAKK52 pKa = 10.16 AWRR55 pKa = 11.84 EE56 pKa = 4.0 RR57 pKa = 11.84 NSDD60 pKa = 3.16 KK61 pKa = 11.23 ARR63 pKa = 11.84 LVSRR67 pKa = 11.84 KK68 pKa = 9.49 LLLAAPLFYY77 pKa = 10.69 SPPGSEE83 pKa = 3.41 TWVRR87 pKa = 11.84 TTAVKK92 pKa = 10.06 PDD94 pKa = 3.54 NASDD98 pKa = 4.45 LIEE101 pKa = 5.38 DD102 pKa = 3.94 VLEE105 pKa = 4.57 DD106 pKa = 3.83 TLKK109 pKa = 10.18 TDD111 pKa = 3.15 ATTTPLTTLSQRR123 pKa = 11.84 VLKK126 pKa = 10.61 ILDD129 pKa = 3.54 SSAGDD134 pKa = 3.39 SLVSQEE140 pKa = 4.84 TILEE144 pKa = 4.08 YY145 pKa = 10.99 LPIYY149 pKa = 10.3 AIIPWRR155 pKa = 11.84 ALVKK159 pKa = 10.65 SGGVTSSEE167 pKa = 4.14 WFSSATGAMTTAIKK181 pKa = 10.54 AADD184 pKa = 3.71 DD185 pKa = 3.8 RR186 pKa = 11.84 LRR188 pKa = 11.84 QADD191 pKa = 3.88 EE192 pKa = 4.1 TVKK195 pKa = 11.1 NDD197 pKa = 3.03 MKK199 pKa = 10.71 TWFAQVDD206 pKa = 3.89 PTEE209 pKa = 4.58 LLTNHH214 pKa = 6.98 LAEE217 pKa = 4.94 IMACALAHH225 pKa = 5.59 SEE227 pKa = 4.11 RR228 pKa = 11.84 VPPFIAASIQSVWMYY243 pKa = 10.48 HH244 pKa = 5.79 GLGTYY249 pKa = 10.03 IFLRR253 pKa = 11.84 EE254 pKa = 3.91 AARR257 pKa = 11.84 TLGLNSPEE265 pKa = 4.29 LLRR268 pKa = 11.84 EE269 pKa = 4.33 LCCPLLNNQIMALVKK284 pKa = 10.73 LMIDD288 pKa = 3.37 EE289 pKa = 4.36 QANNTPVFPYY299 pKa = 10.35 IKK301 pKa = 9.8 IINTNFHH308 pKa = 6.49 SGVSTKK314 pKa = 10.51 RR315 pKa = 11.84 CIPLMYY321 pKa = 10.13 VLRR324 pKa = 11.84 QIICPISPKK333 pKa = 9.36 TKK335 pKa = 9.49 QGEE338 pKa = 4.71 GIWGKK343 pKa = 8.78 MCGVPSLDD351 pKa = 3.17 VRR353 pKa = 11.84 IPLAQAAMRR362 pKa = 11.84 LRR364 pKa = 11.84 RR365 pKa = 11.84 KK366 pKa = 10.03 HH367 pKa = 5.74 ILTVRR372 pKa = 11.84 EE373 pKa = 4.48 GEE375 pKa = 4.24 TSEE378 pKa = 4.78 VMQQILGEE386 pKa = 4.16 IRR388 pKa = 11.84 DD389 pKa = 4.08 TPLGYY394 pKa = 10.4 DD395 pKa = 3.51 DD396 pKa = 4.46 TSSEE400 pKa = 4.1 QEE402 pKa = 4.14 AVDD405 pKa = 4.78 PDD407 pKa = 4.41 DD408 pKa = 5.65 LGNPPAA414 pKa = 5.56
Molecular weight: 45.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.468
IPC2_protein 5.512
IPC_protein 5.499
Toseland 5.626
ProMoST 5.728
Dawson 5.601
Bjellqvist 5.677
Wikipedia 5.537
Rodwell 5.55
Grimsley 5.652
Solomon 5.601
Lehninger 5.575
Nozaki 5.792
DTASelect 5.957
Thurlkill 5.792
EMBOSS 5.753
Sillero 5.868
Patrickios 4.304
IPC_peptide 5.614
IPC2_peptide 5.868
IPC2.peptide.svr19 5.902
Protein with the highest isoelectric point:
>tr|A0A1L3KMY2|A0A1L3KMY2_9VIRU Uncharacterized protein OS=Beihai barnacle virus 7 OX=1922365 PE=4 SV=1
MM1 pKa = 6.75 GQSVSKK7 pKa = 10.28 EE8 pKa = 3.48 IQEE11 pKa = 4.06 RR12 pKa = 11.84 WNTQVQALMHH22 pKa = 6.66 MIRR25 pKa = 11.84 TTIRR29 pKa = 11.84 DD30 pKa = 3.26 TCDD33 pKa = 2.53 ARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 EE38 pKa = 4.18 TVEE41 pKa = 3.97 TIKK44 pKa = 11.33 DD45 pKa = 3.22 EE46 pKa = 4.16 FRR48 pKa = 11.84 RR49 pKa = 11.84 SNVHH53 pKa = 6.01 IEE55 pKa = 3.9 QVYY58 pKa = 10.46 KK59 pKa = 10.54 ILEE62 pKa = 4.18 TAQTVPQPDD71 pKa = 3.78 TQLSARR77 pKa = 11.84 VEE79 pKa = 4.12 NEE81 pKa = 4.29 LFNQDD86 pKa = 2.49 VWNNAGLGTCEE97 pKa = 4.41 PPIKK101 pKa = 9.92 IYY103 pKa = 10.91 AVLSGVIVLLLALSIMFSVLWRR125 pKa = 11.84 IEE127 pKa = 3.89 KK128 pKa = 9.9 AKK130 pKa = 10.6 KK131 pKa = 9.41 RR132 pKa = 11.84 FRR134 pKa = 11.84 RR135 pKa = 11.84 TSSFRR140 pKa = 11.84 PRR142 pKa = 11.84 SILKK146 pKa = 10.18 KK147 pKa = 10.14 PPSPDD152 pKa = 3.22 NVSLSSIRR160 pKa = 11.84 TLYY163 pKa = 10.65
Molecular weight: 18.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.127
IPC2_protein 9.136
IPC_protein 9.224
Toseland 10.072
ProMoST 9.721
Dawson 10.218
Bjellqvist 9.867
Wikipedia 10.365
Rodwell 10.584
Grimsley 10.262
Solomon 10.262
Lehninger 10.248
Nozaki 10.087
DTASelect 9.853
Thurlkill 10.087
EMBOSS 10.452
Sillero 10.145
Patrickios 10.321
IPC_peptide 10.277
IPC2_peptide 8.551
IPC2.peptide.svr19 8.144
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3521
153
1994
586.8
65.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.993 ± 0.732
1.874 ± 0.324
4.487 ± 0.524
5.283 ± 0.481
3.465 ± 0.605
4.857 ± 0.463
2.215 ± 0.238
5.623 ± 0.263
4.232 ± 0.319
11.843 ± 1.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.988 ± 0.152
3.55 ± 0.337
5.993 ± 0.318
3.323 ± 0.292
6.816 ± 0.469
9.458 ± 0.623
7.242 ± 0.622
6.93 ± 0.767
1.448 ± 0.258
3.38 ± 0.347
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here