Candidatus Poseidoniales archaeon
Average proteome isoelectric point is 5.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1593 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3A5WC86|A0A3A5WC86_9ARCH DUF393 domain-containing protein OS=Candidatus Poseidoniales archaeon OX=2163009 GN=DWC06_00590 PE=4 SV=1
MM1 pKa = 7.0 TAKK4 pKa = 9.65 PWVVVAVMAMMCLMPYY20 pKa = 10.45 EE21 pKa = 5.22 SIDD24 pKa = 3.69 LTSLSEE30 pKa = 4.38 DD31 pKa = 3.62 SVKK34 pKa = 10.77 SEE36 pKa = 4.07 SSGNSHH42 pKa = 7.6 EE43 pKa = 4.2 ILLMGNSYY51 pKa = 10.27 VASNSLHH58 pKa = 5.93 NLVDD62 pKa = 3.78 GVMNAASQPANVSTLNGGGMRR83 pKa = 11.84 LSQHH87 pKa = 6.32 ASNVASAGHH96 pKa = 5.21 QWNTTLNNGNWDD108 pKa = 3.58 WVVLQDD114 pKa = 3.83 QSQIPGFPRR123 pKa = 11.84 SQNEE127 pKa = 3.87 WIASKK132 pKa = 10.91 DD133 pKa = 3.59 GAVEE137 pKa = 3.74 LAEE140 pKa = 4.3 VIEE143 pKa = 4.68 DD144 pKa = 3.69 NGGEE148 pKa = 4.43 TVLMMTWGRR157 pKa = 11.84 RR158 pKa = 11.84 DD159 pKa = 3.36 GDD161 pKa = 4.06 SNNAWRR167 pKa = 11.84 FPDD170 pKa = 4.69 FSTMQDD176 pKa = 3.43 EE177 pKa = 4.81 LEE179 pKa = 4.36 SGYY182 pKa = 10.98 LDD184 pKa = 3.45 YY185 pKa = 11.16 RR186 pKa = 11.84 DD187 pKa = 4.66 NISANGGNAWVAPVGLAFEE206 pKa = 5.36 HH207 pKa = 6.2 IHH209 pKa = 6.55 DD210 pKa = 5.15 KK211 pKa = 10.75 IVAEE215 pKa = 4.91 GGTPTNSGNTFYY227 pKa = 11.57 NLYY230 pKa = 9.63 TNDD233 pKa = 3.45 GSHH236 pKa = 7.32 PSLSGSYY243 pKa = 9.38 LAAVVIYY250 pKa = 8.37 ATITGNDD257 pKa = 3.7 PVGLSHH263 pKa = 6.66 STSLSNGLVLEE274 pKa = 4.53 LQQAAAATVFNEE286 pKa = 4.48 TSHH289 pKa = 7.92 LDD291 pKa = 3.96 YY292 pKa = 10.55 PWQTTNQSPNNASIIQSEE310 pKa = 4.04 FLAWEE315 pKa = 4.68 SISSYY320 pKa = 11.05 GSFRR324 pKa = 11.84 TEE326 pKa = 3.16 WGNAIAASSTGDD338 pKa = 3.59 YY339 pKa = 10.82 YY340 pKa = 11.96 VLGSSKK346 pKa = 10.2 EE347 pKa = 3.9 DD348 pKa = 3.19 HH349 pKa = 6.78 YY350 pKa = 11.74 LGTNCHH356 pKa = 5.93 MNHH359 pKa = 7.0 AGSTSYY365 pKa = 11.4 AQTFLAKK372 pKa = 10.06 FDD374 pKa = 4.45 NNSNCLWLINTYY386 pKa = 11.31 SNTQFNFHH394 pKa = 6.1 YY395 pKa = 10.49 QPTHH399 pKa = 7.05 RR400 pKa = 11.84 IGLDD404 pKa = 3.18 SSDD407 pKa = 3.86 NIYY410 pKa = 10.96 VIFSMHH416 pKa = 6.57 SDD418 pKa = 3.07 AAGQQTGSFGNAQFTTNNQHH438 pKa = 6.03 GSTVLLSKK446 pKa = 10.83 VNSNGSVEE454 pKa = 4.29 WIRR457 pKa = 11.84 NNGDD461 pKa = 3.81 GKK463 pKa = 9.24 CHH465 pKa = 6.78 CALAVDD471 pKa = 4.15 NNDD474 pKa = 3.8 NITISFNTGNNNMGLKK490 pKa = 7.89 MQKK493 pKa = 9.6 IDD495 pKa = 3.75 TNGSLLFNYY504 pKa = 9.78 QLGGAGSGQDD514 pKa = 2.78 RR515 pKa = 11.84 AFSHH519 pKa = 6.46 GLVADD524 pKa = 4.0 SSDD527 pKa = 4.05 NIYY530 pKa = 9.97 MAGSYY535 pKa = 10.2 YY536 pKa = 10.09 GTVSFPSLGSYY547 pKa = 7.8 TCQNCYY553 pKa = 9.05 TGYY556 pKa = 9.96 VAKK559 pKa = 10.44 FDD561 pKa = 5.0 SNLTAQWVEE570 pKa = 3.7 IAEE573 pKa = 4.46 TSSSSSEE580 pKa = 3.73 IEE582 pKa = 4.68 GIDD585 pKa = 3.52 VNADD589 pKa = 3.29 DD590 pKa = 5.43 EE591 pKa = 4.84 IFVTGTFYY599 pKa = 11.3 SEE601 pKa = 4.62 LVFNGTTMDD610 pKa = 3.45 SGGNEE615 pKa = 3.64 NGFAAKK621 pKa = 10.1 INSTGGWEE629 pKa = 3.63 WGVRR633 pKa = 11.84 LGCTCTIIPNDD644 pKa = 4.7 LILDD648 pKa = 3.77 SRR650 pKa = 11.84 NNVYY654 pKa = 10.18 IAGRR658 pKa = 11.84 HH659 pKa = 5.52 HH660 pKa = 6.26 GTMSFGGIQLTQSGTMGDD678 pKa = 3.84 GFILKK683 pKa = 10.19 LNSTGTGIWGKK694 pKa = 10.48 SFGGNSEE701 pKa = 5.37 DD702 pKa = 3.8 RR703 pKa = 11.84 FQSLFFDD710 pKa = 4.86 EE711 pKa = 5.14 ISGNLYY717 pKa = 10.17 TIGTFQTTAVSGQFLKK733 pKa = 10.32 TSAGEE738 pKa = 3.82 GDD740 pKa = 4.01 VFVGIMSKK748 pKa = 10.62 DD749 pKa = 3.49 YY750 pKa = 11.4 DD751 pKa = 4.28 GDD753 pKa = 5.0 DD754 pKa = 3.37 ITDD757 pKa = 3.54 KK758 pKa = 11.0 FDD760 pKa = 4.54 YY761 pKa = 11.0 DD762 pKa = 5.36 DD763 pKa = 6.37 DD764 pKa = 6.58 DD765 pKa = 6.48 DD766 pKa = 5.96 FINDD770 pKa = 4.26 PLDD773 pKa = 3.47 MCHH776 pKa = 6.41 YY777 pKa = 10.37 SSIGFKK783 pKa = 9.6 STGSSDD789 pKa = 3.62 HH790 pKa = 7.45 DD791 pKa = 4.27 SDD793 pKa = 4.4 GCDD796 pKa = 4.86 DD797 pKa = 3.76 EE798 pKa = 6.06 TEE800 pKa = 5.47 DD801 pKa = 5.83 FDD803 pKa = 7.24 DD804 pKa = 6.99 DD805 pKa = 4.99 NDD807 pKa = 4.38 LLNDD811 pKa = 4.15 TLDD814 pKa = 3.83 NCPKK818 pKa = 10.87 GMTGWIRR825 pKa = 11.84 TNASDD830 pKa = 5.8 LDD832 pKa = 4.39 DD833 pKa = 5.6 DD834 pKa = 4.55 GCMDD838 pKa = 4.82 ALEE841 pKa = 5.87 DD842 pKa = 4.69 YY843 pKa = 11.4 DD844 pKa = 5.06 DD845 pKa = 5.95 DD846 pKa = 5.02 NDD848 pKa = 4.05 GFEE851 pKa = 5.45 DD852 pKa = 4.32 YY853 pKa = 11.05 EE854 pKa = 4.89 DD855 pKa = 3.83 YY856 pKa = 10.91 CSRR859 pKa = 11.84 IPGNSTFEE867 pKa = 4.11 FEE869 pKa = 4.83 KK870 pKa = 10.83 GCPDD874 pKa = 3.5 ADD876 pKa = 3.53 GDD878 pKa = 4.09 GRR880 pKa = 11.84 PDD882 pKa = 3.12 ILDD885 pKa = 3.86 PFKK888 pKa = 10.96 DD889 pKa = 5.42 DD890 pKa = 3.36 PTEE893 pKa = 3.91 WSDD896 pKa = 3.27 TDD898 pKa = 3.74 GDD900 pKa = 4.52 GVGDD904 pKa = 3.95 NSDD907 pKa = 4.51 AFPSDD912 pKa = 2.79 ATQQYY917 pKa = 8.14 DD918 pKa = 3.61 TDD920 pKa = 4.02 GDD922 pKa = 4.15 TYY924 pKa = 11.52 GDD926 pKa = 3.93 EE927 pKa = 4.42 EE928 pKa = 4.75 FGNAGDD934 pKa = 3.63 SCPTVFGNSTIDD946 pKa = 3.65 RR947 pKa = 11.84 YY948 pKa = 11.4 GCLDD952 pKa = 3.36 TDD954 pKa = 3.77 GDD956 pKa = 3.97 GWSDD960 pKa = 3.56 EE961 pKa = 4.74 GDD963 pKa = 4.38 DD964 pKa = 4.64 FPLDD968 pKa = 3.72 PTEE971 pKa = 5.24 HH972 pKa = 7.14 LDD974 pKa = 3.89 SDD976 pKa = 3.99 SDD978 pKa = 4.37 GYY980 pKa = 10.19 PDD982 pKa = 4.79 SSDD985 pKa = 4.93 DD986 pKa = 4.01 FPFDD990 pKa = 3.23 PTQWKK995 pKa = 10.65 DD996 pKa = 3.07 SDD998 pKa = 3.67 GDD1000 pKa = 4.21 GYY1002 pKa = 11.54 GDD1004 pKa = 3.66 NPQGNLGDD1012 pKa = 5.02 AYY1014 pKa = 10.11 PNDD1017 pKa = 3.52 ASRR1020 pKa = 11.84 YY1021 pKa = 8.47 ADD1023 pKa = 3.49 SDD1025 pKa = 3.5 RR1026 pKa = 11.84 DD1027 pKa = 4.24 GIDD1030 pKa = 3.53 DD1031 pKa = 3.64 QADD1034 pKa = 3.6 AFPYY1038 pKa = 10.72 DD1039 pKa = 3.29 PTQWVDD1045 pKa = 2.86 ADD1047 pKa = 3.93 GDD1049 pKa = 4.21 GMGDD1053 pKa = 3.14 NPMGIGADD1061 pKa = 4.04 KK1062 pKa = 10.96 FPDD1065 pKa = 5.43 DD1066 pKa = 3.64 PTQWGDD1072 pKa = 2.9 IDD1074 pKa = 4.28 GDD1076 pKa = 4.29 GYY1078 pKa = 11.56 GDD1080 pKa = 3.58 NSTGNNPDD1088 pKa = 4.0 AFPTDD1093 pKa = 3.25 ATQWSDD1099 pKa = 2.96 MDD1101 pKa = 3.99 GDD1103 pKa = 4.52 GYY1105 pKa = 11.38 GDD1107 pKa = 3.55 NPAGRR1112 pKa = 11.84 LYY1114 pKa = 11.03 DD1115 pKa = 4.39 LFPEE1119 pKa = 5.55 NPTQWEE1125 pKa = 4.18 DD1126 pKa = 3.29 ADD1128 pKa = 4.58 GDD1130 pKa = 4.28 GLGDD1134 pKa = 3.73 NQSGTDD1140 pKa = 3.64 ADD1142 pKa = 4.43 PYY1144 pKa = 11.63 LNDD1147 pKa = 4.53 FDD1149 pKa = 5.09 NDD1151 pKa = 4.18 GYY1153 pKa = 11.61 NDD1155 pKa = 4.4 TIDD1158 pKa = 3.36 ILPRR1162 pKa = 11.84 VASPGDD1168 pKa = 3.84 LDD1170 pKa = 4.77 NDD1172 pKa = 3.46 KK1173 pKa = 11.57 CMDD1176 pKa = 4.08 GDD1178 pKa = 4.37 DD1179 pKa = 4.87 AFWDD1183 pKa = 3.95 NPLEE1187 pKa = 4.41 CLDD1190 pKa = 3.94 TDD1192 pKa = 4.19 GDD1194 pKa = 4.37 KK1195 pKa = 10.88 IGNNADD1201 pKa = 3.73 SDD1203 pKa = 4.14 DD1204 pKa = 6.18 DD1205 pKa = 4.1 NDD1207 pKa = 3.18 KK1208 pKa = 8.93 WTDD1211 pKa = 3.1 ADD1213 pKa = 4.31 EE1214 pKa = 4.41 IRR1216 pKa = 11.84 AGTDD1220 pKa = 3.2 PLDD1223 pKa = 4.47 PNSTPVDD1230 pKa = 3.51 SFEE1233 pKa = 4.05 IQIGNIGLGAWDD1245 pKa = 5.16 LIGIFGGVPIFAWIAFGFVTRR1266 pKa = 11.84 SARR1269 pKa = 11.84 CARR1272 pKa = 11.84 YY1273 pKa = 9.68 EE1274 pKa = 4.07 EE1275 pKa = 4.28 QLNEE1279 pKa = 3.96 ANSRR1283 pKa = 11.84 EE1284 pKa = 4.13 EE1285 pKa = 4.19 LEE1287 pKa = 4.29 QVALRR1292 pKa = 11.84 WEE1294 pKa = 4.16 YY1295 pKa = 11.72 SLMLRR1300 pKa = 11.84 LLGPHH1305 pKa = 5.33 QGIRR1309 pKa = 11.84 LEE1311 pKa = 4.1 RR1312 pKa = 11.84 LRR1314 pKa = 11.84 SEE1316 pKa = 5.18 LDD1318 pKa = 3.44 DD1319 pKa = 4.06 KK1320 pKa = 11.73 FEE1322 pKa = 4.25 NAEE1325 pKa = 4.08 LLMAAEE1331 pKa = 4.98 DD1332 pKa = 4.39 FEE1334 pKa = 5.1 PMTEE1338 pKa = 4.11 VEE1340 pKa = 4.12 QAPLVEE1346 pKa = 5.41 AEE1348 pKa = 4.54 QKK1350 pKa = 10.51 DD1351 pKa = 4.23 LPHH1354 pKa = 8.04 IDD1356 pKa = 4.35 MIPTIDD1362 pKa = 3.78 IAPDD1366 pKa = 3.15 QTDD1369 pKa = 3.18 EE1370 pKa = 5.8 FGYY1373 pKa = 10.59 SWLNYY1378 pKa = 10.0 NGEE1381 pKa = 3.59 NWYY1384 pKa = 10.4 RR1385 pKa = 11.84 GQNDD1389 pKa = 4.12 TEE1391 pKa = 4.35 WTKK1394 pKa = 11.04 HH1395 pKa = 4.77 EE1396 pKa = 4.21 EE1397 pKa = 3.99
Molecular weight: 152.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.679
IPC2_protein 3.656
IPC_protein 3.719
Toseland 3.478
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.541
Grimsley 3.389
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.126
Thurlkill 3.528
EMBOSS 3.681
Sillero 3.846
Patrickios 1.036
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|A0A3A5WC38|A0A3A5WC38_9ARCH DNA topoisomerase (ATP-hydrolyzing) OS=Candidatus Poseidoniales archaeon OX=2163009 GN=DWC06_00685 PE=3 SV=1
MM1 pKa = 7.43 SRR3 pKa = 11.84 NKK5 pKa = 9.61 PAAKK9 pKa = 10.08 KK10 pKa = 10.0 IRR12 pKa = 11.84 LLKK15 pKa = 10.49 AGKK18 pKa = 7.79 QNRR21 pKa = 11.84 RR22 pKa = 11.84 VPVWVMLKK30 pKa = 9.3 TSRR33 pKa = 11.84 NTVSHH38 pKa = 7.37 PKK40 pKa = 7.84 RR41 pKa = 11.84 HH42 pKa = 4.93 HH43 pKa = 5.04 WRR45 pKa = 11.84 RR46 pKa = 11.84 SKK48 pKa = 10.72 LQRR51 pKa = 3.58
Molecular weight: 6.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1593
0
1593
599017
49
4673
376.0
41.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.681 ± 0.063
1.145 ± 0.022
7.099 ± 0.088
7.049 ± 0.078
3.475 ± 0.043
8.196 ± 0.062
2.168 ± 0.035
6.72 ± 0.049
3.975 ± 0.076
9.013 ± 0.091
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.932 ± 0.029
4.286 ± 0.066
4.329 ± 0.037
3.034 ± 0.033
4.654 ± 0.071
7.452 ± 0.078
5.597 ± 0.078
6.885 ± 0.039
1.92 ± 0.033
2.392 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here