Lactococcus phage bIL285

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q77JM2|Q77JM2_9CAUD Orf22 OS=Lactococcus phage bIL285 OX=151535 GN=orf22 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 9.96CDD4 pKa = 3.13KK5 pKa = 10.52CGNEE9 pKa = 4.13IDD11 pKa = 4.27CDD13 pKa = 3.84CMGCHH18 pKa = 6.09EE19 pKa = 4.92CHH21 pKa = 6.92PEE23 pKa = 4.08YY24 pKa = 10.65TCEE27 pKa = 4.03TCGFCHH33 pKa = 6.81IDD35 pKa = 2.97GWEE38 pKa = 4.39AGACWSLANDD48 pKa = 4.05PDD50 pKa = 4.18YY51 pKa = 11.63DD52 pKa = 3.94PFDD55 pKa = 3.3II56 pKa = 5.77

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9AZZ6|Q9AZZ6_9CAUD Orf33 OS=Lactococcus phage bIL285 OX=151535 GN=orf33 PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 10.49SSWKK6 pKa = 9.97KK7 pKa = 9.68QRR9 pKa = 11.84QTTKK13 pKa = 10.09KK14 pKa = 10.39RR15 pKa = 11.84QIKK18 pKa = 8.04WLRR21 pKa = 11.84IKK23 pKa = 10.59HH24 pKa = 5.97RR25 pKa = 11.84LIKK28 pKa = 10.52SYY30 pKa = 10.16TPNIEE35 pKa = 4.28AFIKK39 pKa = 10.51LFNDD43 pKa = 3.37IKK45 pKa = 10.68IAVSNISMAIGKK57 pKa = 9.78AFIDD61 pKa = 3.49IGKK64 pKa = 9.23SLHH67 pKa = 6.59PSNTTVSAIDD77 pKa = 3.66VPPIGLGADD86 pKa = 3.27MRR88 pKa = 11.84CYY90 pKa = 10.94VEE92 pKa = 5.83DD93 pKa = 3.66IPGGRR98 pKa = 11.84SS99 pKa = 2.88

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

11072

42

894

178.6

20.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.015 ± 0.35

0.668 ± 0.136

6.422 ± 0.203

7.298 ± 0.396

4.462 ± 0.23

6.196 ± 0.47

1.427 ± 0.15

7.027 ± 0.245

9.095 ± 0.383

8.409 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.61 ± 0.148

5.546 ± 0.287

2.98 ± 0.19

4.146 ± 0.236

3.658 ± 0.258

6.693 ± 0.281

6.133 ± 0.28

5.717 ± 0.242

1.508 ± 0.151

3.992 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski