Asaia bogorensis
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3273 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A060QKR7|A0A060QKR7_9PROT FGGY_N domain-containing protein OS=Asaia bogorensis OX=91915 GN=ASAP_2101 PE=4 SV=1
MM1 pKa = 7.84 NDD3 pKa = 2.66 TSMNSVQIATDD14 pKa = 4.09 PAAHH18 pKa = 7.27 AYY20 pKa = 10.27 AIIDD24 pKa = 3.46 ASGEE28 pKa = 4.25 VVNRR32 pKa = 11.84 IVWDD36 pKa = 3.71 GRR38 pKa = 11.84 GNLILPTGQYY48 pKa = 8.49 WVEE51 pKa = 4.51 DD52 pKa = 4.03 DD53 pKa = 4.04 ASAYY57 pKa = 9.84 PIGSTYY63 pKa = 10.57 KK64 pKa = 10.79 APTPTSGTAGG74 pKa = 3.28
Molecular weight: 7.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.852
IPC2_protein 4.164
IPC_protein 4.012
Toseland 3.808
ProMoST 4.215
Dawson 4.024
Bjellqvist 4.19
Wikipedia 4.012
Rodwell 3.846
Grimsley 3.732
Solomon 3.999
Lehninger 3.961
Nozaki 4.164
DTASelect 4.406
Thurlkill 3.897
EMBOSS 4.012
Sillero 4.139
Patrickios 1.977
IPC_peptide 3.999
IPC2_peptide 4.113
IPC2.peptide.svr19 4.029
Protein with the highest isoelectric point:
>tr|A0A060QCE0|A0A060QCE0_9PROT Oxidoreductase OS=Asaia bogorensis OX=91915 GN=ASAP_0744 PE=4 SV=1
MM1 pKa = 7.39 RR2 pKa = 11.84 RR3 pKa = 11.84 AIVFVLSVSANQNVIVTNQVRR24 pKa = 11.84 TLAFALRR31 pKa = 11.84 GRR33 pKa = 11.84 LHH35 pKa = 6.69 HH36 pKa = 6.47 ATPISS41 pKa = 3.35
Molecular weight: 4.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.386
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3273
0
3273
1008756
37
2114
308.2
33.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.834 ± 0.058
1.027 ± 0.014
5.537 ± 0.035
5.122 ± 0.044
3.495 ± 0.027
8.48 ± 0.04
2.494 ± 0.02
5.138 ± 0.028
2.767 ± 0.032
10.459 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.569 ± 0.021
2.697 ± 0.033
5.45 ± 0.033
3.594 ± 0.025
7.204 ± 0.046
6.044 ± 0.039
5.692 ± 0.036
6.743 ± 0.034
1.384 ± 0.017
2.255 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here