Roseivirga ehrenbergii (strain DSM 102268 / JCM 13514 / KCTC 12282 / NCIMB 14502 / KMM 6017)
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3632 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A150XLH7|A0A150XLH7_ROSEK POTRA domain-containing protein OS=Roseivirga ehrenbergii (strain DSM 102268 / JCM 13514 / KCTC 12282 / NCIMB 14502 / KMM 6017) OX=279360 GN=MB14_17200 PE=4 SV=1
MM1 pKa = 6.9 VRR3 pKa = 11.84 KK4 pKa = 7.9 TRR6 pKa = 11.84 ILLLLLGITFSVLTQAQNFTRR27 pKa = 11.84 HH28 pKa = 4.91 NWYY31 pKa = 10.37 FSNNDD36 pKa = 2.85 QALIFGKK43 pKa = 9.75 EE44 pKa = 3.39 QAARR48 pKa = 11.84 SFIADD53 pKa = 3.49 GKK55 pKa = 9.93 IAQTNAGEE63 pKa = 4.52 KK64 pKa = 9.13 LTATDD69 pKa = 3.89 PTTGDD74 pKa = 3.67 LLFYY78 pKa = 10.88 SDD80 pKa = 5.7 GINIYY85 pKa = 10.31 DD86 pKa = 3.54 ATHH89 pKa = 7.03 AIMEE93 pKa = 4.74 NGTGLTTDD101 pKa = 3.85 PNGIQPLALSPVPGVGNEE119 pKa = 3.23 SRR121 pKa = 11.84 YY122 pKa = 9.77 YY123 pKa = 9.27 VFHH126 pKa = 7.11 RR127 pKa = 11.84 NVTGEE132 pKa = 3.79 ILYY135 pKa = 9.12 TLVDD139 pKa = 3.71 MSSQGNRR146 pKa = 11.84 VDD148 pKa = 4.13 GPPAGAVVIGSKK160 pKa = 9.42 NQPTGIISRR169 pKa = 11.84 GDD171 pKa = 3.16 GMLTVASGDD180 pKa = 3.41 MTQFWLVTQNSTNGNFEE197 pKa = 3.59 IHH199 pKa = 7.25 EE200 pKa = 4.43 IPVAGQPFDD209 pKa = 3.75 PQGAPTVLTANVQAAHH225 pKa = 7.02 LSLNPMGTQIAVRR238 pKa = 11.84 PSNNVNIQILDD249 pKa = 3.71 FNRR252 pKa = 11.84 DD253 pKa = 3.28 TPEE256 pKa = 3.78 LMFNRR261 pKa = 11.84 SVANSFVPNQTLTGSIGWSNDD282 pKa = 2.38 GTKK285 pKa = 10.48 LYY287 pKa = 10.67 FSRR290 pKa = 11.84 NLANSTSGNLYY301 pKa = 10.41 RR302 pKa = 11.84 IDD304 pKa = 4.88 LSDD307 pKa = 3.41 PMASVEE313 pKa = 4.32 PVFSNPIGASQSVMLAPDD331 pKa = 3.03 SSLYY335 pKa = 10.51 HH336 pKa = 6.62 IYY338 pKa = 10.61 KK339 pKa = 8.39 EE340 pKa = 4.29 TAAGPRR346 pKa = 11.84 LLARR350 pKa = 11.84 INQPDD355 pKa = 3.72 SALTALDD362 pKa = 4.17 YY363 pKa = 11.13 EE364 pKa = 4.57 PSLFDD369 pKa = 3.82 EE370 pKa = 5.39 FDD372 pKa = 3.59 PASDD376 pKa = 3.81 YY377 pKa = 10.59 FQQFMPPAAIGPTVTISDD395 pKa = 4.07 DD396 pKa = 3.47 GDD398 pKa = 3.68 YY399 pKa = 11.58 CMNTPVQFYY408 pKa = 11.34 VNIDD412 pKa = 3.55 PSTAIPTSYY421 pKa = 10.51 SWDD424 pKa = 3.33 IQPFGITSNAIAPIFTFDD442 pKa = 3.24 QAGMYY447 pKa = 7.39 MATLTVEE454 pKa = 4.25 FDD456 pKa = 4.19 GIGTVMSNTLMGEE469 pKa = 3.51 IMEE472 pKa = 4.83 NDD474 pKa = 3.31 IQVSLQDD481 pKa = 3.37 TTICEE486 pKa = 4.58 GEE488 pKa = 4.5 TLTLDD493 pKa = 4.69 AEE495 pKa = 4.44 PQSGGQGQQGGATGGPFEE513 pKa = 4.4 YY514 pKa = 9.96 RR515 pKa = 11.84 WNTGEE520 pKa = 4.18 TTSSIDD526 pKa = 3.17 VTDD529 pKa = 4.11 AGNYY533 pKa = 6.32 WVVITPTTGCPIYY546 pKa = 9.66 EE547 pKa = 4.17 TVEE550 pKa = 3.94 VKK552 pKa = 10.8 VYY554 pKa = 11.03 GDD556 pKa = 3.99 EE557 pKa = 4.44 NPTANIWYY565 pKa = 8.85 FGNGAGIDD573 pKa = 3.89 FNEE576 pKa = 4.31 VDD578 pKa = 4.56 GLAPPPRR585 pKa = 11.84 SITTAHH591 pKa = 6.83 AMNAPEE597 pKa = 4.36 GTSTISDD604 pKa = 3.51 ANGQVLFYY612 pKa = 11.07 TNGTSVWNADD622 pKa = 3.4 NGVMPNGTKK631 pKa = 9.86 IGGDD635 pKa = 3.57 STSTQAVVIVPFEE648 pKa = 4.52 DD649 pKa = 5.32 DD650 pKa = 3.02 EE651 pKa = 4.68 TLFYY655 pKa = 11.31 VFTTEE660 pKa = 4.73 EE661 pKa = 3.92 VHH663 pKa = 7.41 GDD665 pKa = 3.14 NTYY668 pKa = 8.38 MLKK671 pKa = 10.75 YY672 pKa = 10.47 SVVDD676 pKa = 4.09 LKK678 pKa = 11.23 EE679 pKa = 4.44 DD680 pKa = 3.44 NGLGAVISKK689 pKa = 10.96 DD690 pKa = 3.06 NVLFTRR696 pKa = 11.84 STEE699 pKa = 3.84 KK700 pKa = 10.98 LIALEE705 pKa = 4.14 GAGGYY710 pKa = 8.97 WLMAHH715 pKa = 7.36 EE716 pKa = 4.92 YY717 pKa = 8.48 GTNTFRR723 pKa = 11.84 AYY725 pKa = 9.96 PITNQGIGQPILSSVGSVHH744 pKa = 7.26 SINDD748 pKa = 3.7 ALSGQAGMKK757 pKa = 10.41 FSGDD761 pKa = 3.23 RR762 pKa = 11.84 LRR764 pKa = 11.84 IAVALIEE771 pKa = 4.38 GNEE774 pKa = 4.23 DD775 pKa = 3.53 YY776 pKa = 11.62 VEE778 pKa = 4.45 LFSFDD783 pKa = 4.07 ANTGEE788 pKa = 4.41 VTDD791 pKa = 5.08 LLYY794 pKa = 10.89 QIDD797 pKa = 4.28 LGEE800 pKa = 4.83 GGPALNDD807 pKa = 3.12 QVYY810 pKa = 10.2 DD811 pKa = 3.43 VHH813 pKa = 7.54 FSTGGDD819 pKa = 3.17 KK820 pKa = 11.09 LFATMNNRR828 pKa = 11.84 NGGAPGGRR836 pKa = 11.84 ILEE839 pKa = 3.97 YY840 pKa = 10.44 RR841 pKa = 11.84 IDD843 pKa = 3.78 SASTEE848 pKa = 4.25 VTRR851 pKa = 11.84 LASKK855 pKa = 9.88 TDD857 pKa = 2.92 ISTSSTVTVNYY868 pKa = 10.34 GAIQTGPDD876 pKa = 3.31 GQLYY880 pKa = 9.53 VAMEE884 pKa = 4.19 IPGNPAGTPFIGSINVNEE902 pKa = 4.1 DD903 pKa = 3.04 TAAVSSFNQMQVPLTTGNSRR923 pKa = 11.84 LGLPNFVQNNVNPPMEE939 pKa = 4.93 PSMSAPDD946 pKa = 3.64 STCVEE951 pKa = 4.04 EE952 pKa = 5.47 RR953 pKa = 11.84 IEE955 pKa = 3.92 LSGVGTSDD963 pKa = 2.73 IDD965 pKa = 3.54 EE966 pKa = 4.6 FLWTIIDD973 pKa = 3.99 DD974 pKa = 4.22 ANTTVFSASGQDD986 pKa = 3.17 TAYY989 pKa = 10.8 VFPQGQAGRR998 pKa = 11.84 FDD1000 pKa = 3.17 ISLNISNRR1008 pKa = 11.84 CGFDD1012 pKa = 2.92 TTFVQTIDD1020 pKa = 3.5 VFEE1023 pKa = 4.43 IPPRR1027 pKa = 11.84 PAVPTAVVLCEE1038 pKa = 4.31 GSSHH1042 pKa = 7.47 DD1043 pKa = 4.44 LDD1045 pKa = 4.52 ALGGEE1050 pKa = 4.59 PDD1052 pKa = 4.56 DD1053 pKa = 4.27 PTLSFEE1059 pKa = 4.16 WTNSQGAVVSNSRR1072 pKa = 11.84 YY1073 pKa = 8.85 YY1074 pKa = 10.93 SVTEE1078 pKa = 3.8 EE1079 pKa = 4.26 EE1080 pKa = 5.27 IYY1082 pKa = 10.6 TITITSVGGGGCVSSAEE1099 pKa = 4.35 VYY1101 pKa = 9.81 AAPPFEE1107 pKa = 5.63 IEE1109 pKa = 4.59 LPEE1112 pKa = 4.05 TANLCEE1118 pKa = 4.55 GEE1120 pKa = 4.66 SLTLDD1125 pKa = 3.54 PNVNANNYY1133 pKa = 7.51 IWTRR1137 pKa = 11.84 INPDD1141 pKa = 2.82 NSTTTLPNQRR1151 pKa = 11.84 TATVDD1156 pKa = 3.17 SSVPGIFTYY1165 pKa = 10.59 VVSIEE1170 pKa = 4.52 DD1171 pKa = 4.86 PIEE1174 pKa = 4.39 PGCFANDD1181 pKa = 3.49 STVVTINGSPNLSLVSSQDD1200 pKa = 3.04 ASCPQVGPTPDD1211 pKa = 3.34 GEE1213 pKa = 4.64 FVFNIAGTGNYY1224 pKa = 9.98 SYY1226 pKa = 10.07 TVSNATGATVAQSNSVNGPNDD1247 pKa = 3.25 VSVTGLDD1254 pKa = 3.33 GGIYY1258 pKa = 9.9 SVNVTDD1264 pKa = 5.45 NSTNCTDD1271 pKa = 3.44 EE1272 pKa = 3.99 LTGIEE1277 pKa = 3.89 IQNNNFNIIEE1287 pKa = 4.24 PVVTADD1293 pKa = 3.79 ATCDD1297 pKa = 3.42 TADD1300 pKa = 3.4 GSITITLDD1308 pKa = 3.4 DD1309 pKa = 4.29 ASVFPVSYY1317 pKa = 11.04 VLTRR1321 pKa = 11.84 DD1322 pKa = 3.36 EE1323 pKa = 5.54 AGFTPLSGNAVANGFVISGLEE1344 pKa = 3.8 GGTYY1348 pKa = 10.08 SIEE1351 pKa = 4.23 VTSTALGCTATASGIVVNQPTDD1373 pKa = 3.47 VQNLQISGTTEE1384 pKa = 3.57 VCDD1387 pKa = 3.69 ISPSTTLTATSNNGTNYY1404 pKa = 8.87 TWIGPSGPAGNTASITVTEE1423 pKa = 4.33 SGTYY1427 pKa = 8.94 TVTADD1432 pKa = 3.81 APGVACPTSEE1442 pKa = 4.26 SVIVTLTVQPDD1453 pKa = 3.45 VEE1455 pKa = 4.82 IIQIGDD1461 pKa = 3.5 VCDD1464 pKa = 3.66 GTLVLEE1470 pKa = 4.17 AQINNEE1476 pKa = 4.26 NPTATYY1482 pKa = 10.72 NFAWSTGEE1490 pKa = 3.77 NTRR1493 pKa = 11.84 SITVSADD1500 pKa = 2.66 GTYY1503 pKa = 11.02 DD1504 pKa = 3.28 VTVRR1508 pKa = 11.84 EE1509 pKa = 4.77 AGDD1512 pKa = 3.62 LTCTGTYY1519 pKa = 10.12 SEE1521 pKa = 5.13 TIDD1524 pKa = 3.89 FPEE1527 pKa = 4.28 EE1528 pKa = 3.86 LEE1530 pKa = 4.27 ATLSSSPACDD1540 pKa = 3.71 DD1541 pKa = 3.84 GQPITLTVDD1550 pKa = 3.5 VLSGNATNFTWTRR1563 pKa = 11.84 NGQAAGTGNPLVVNEE1578 pKa = 4.22 EE1579 pKa = 3.59 GSYY1582 pKa = 9.66 TVTISDD1588 pKa = 3.6 NSGCSIEE1595 pKa = 4.43 RR1596 pKa = 11.84 SILIRR1601 pKa = 11.84 RR1602 pKa = 11.84 SVIPEE1607 pKa = 3.85 GLLPDD1612 pKa = 3.97 LEE1614 pKa = 5.22 YY1615 pKa = 11.46 YY1616 pKa = 10.83 CSTSSNVLTVTAGIGFEE1633 pKa = 4.29 TYY1635 pKa = 8.99 EE1636 pKa = 4.15 WTLDD1640 pKa = 3.86 GQPLAHH1646 pKa = 7.06 SDD1648 pKa = 3.36 QDD1650 pKa = 4.04 LVIAAPGIYY1659 pKa = 9.26 QVTMTTATGCVRR1671 pKa = 11.84 VDD1673 pKa = 3.11 QMEE1676 pKa = 4.73 IIEE1679 pKa = 4.46 SCDD1682 pKa = 3.52 PVILAPNVIVPEE1694 pKa = 4.3 GNPPNNVFSVVPNGFVSDD1712 pKa = 3.81 FQIFIYY1718 pKa = 10.35 SRR1720 pKa = 11.84 WGEE1723 pKa = 4.31 LIYY1726 pKa = 10.62 QSSALEE1732 pKa = 4.09 FQWNGTFNGQLVPIGTYY1749 pKa = 9.6 PYY1751 pKa = 10.01 VIKK1754 pKa = 10.5 FRR1756 pKa = 11.84 SRR1758 pKa = 11.84 FQPEE1762 pKa = 3.49 RR1763 pKa = 11.84 GEE1765 pKa = 4.07 FEE1767 pKa = 3.74 QRR1769 pKa = 11.84 GAITVVRR1776 pKa = 4.35
Molecular weight: 189.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.846
IPC_protein 3.872
Toseland 3.656
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.757
Rodwell 3.694
Grimsley 3.567
Solomon 3.846
Lehninger 3.795
Nozaki 3.948
DTASelect 4.177
Thurlkill 3.694
EMBOSS 3.77
Sillero 3.986
Patrickios 0.655
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.87
Protein with the highest isoelectric point:
>tr|A0A150X0B7|A0A150X0B7_ROSEK Peptidase OS=Roseivirga ehrenbergii (strain DSM 102268 / JCM 13514 / KCTC 12282 / NCIMB 14502 / KMM 6017) OX=279360 GN=MB14_08900 PE=4 SV=1
MM1 pKa = 7.38 TFLFEE6 pKa = 5.33 LYY8 pKa = 9.78 IKK10 pKa = 10.57 RR11 pKa = 11.84 LLGKK15 pKa = 9.38 YY16 pKa = 9.33 RR17 pKa = 11.84 YY18 pKa = 9.24 RR19 pKa = 11.84 RR20 pKa = 11.84 KK21 pKa = 10.27 SLLWHH26 pKa = 6.81 LSRR29 pKa = 11.84 GVNCGKK35 pKa = 9.56 PLKK38 pKa = 10.4 RR39 pKa = 11.84 LMEE42 pKa = 4.32 TLCSIAHH49 pKa = 6.1 GLNRR53 pKa = 11.84 GLFEE57 pKa = 6.39 CITLGNGFNHH67 pKa = 6.29 FNWVVGLLILPQRR80 pKa = 11.84 ALSSAKK86 pKa = 10.09 GKK88 pKa = 10.01 LVQRR92 pKa = 11.84 QQ93 pKa = 3.09
Molecular weight: 10.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.94
IPC_protein 10.789
Toseland 10.965
ProMoST 10.701
Dawson 11.038
Bjellqvist 10.774
Wikipedia 11.272
Rodwell 11.301
Grimsley 11.082
Solomon 11.199
Lehninger 11.155
Nozaki 10.95
DTASelect 10.76
Thurlkill 10.95
EMBOSS 11.374
Sillero 10.979
Patrickios 11.052
IPC_peptide 11.199
IPC2_peptide 9.955
IPC2.peptide.svr19 8.331
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3632
0
3632
1259309
54
4957
346.7
38.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.802 ± 0.037
0.642 ± 0.012
5.576 ± 0.048
6.917 ± 0.05
5.185 ± 0.035
6.809 ± 0.047
1.713 ± 0.021
7.402 ± 0.032
7.01 ± 0.056
9.483 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.412 ± 0.025
5.524 ± 0.037
3.502 ± 0.023
3.509 ± 0.025
3.847 ± 0.029
6.737 ± 0.042
5.57 ± 0.061
6.46 ± 0.029
1.091 ± 0.017
3.809 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here