Kwoniella mangroviensis CBS 10435
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8428 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B9IP46|A0A1B9IP46_9TREE Elongator complex protein 5 OS=Kwoniella mangroviensis CBS 10435 OX=1331196 GN=L486_04804 PE=3 SV=1
MM1 pKa = 7.05 QLSIIPFLTILSILFPTSSAQLDD24 pKa = 3.8 TDD26 pKa = 4.52 IICPDD31 pKa = 3.4 GSLTEE36 pKa = 4.4 YY37 pKa = 8.58 LTSMMDD43 pKa = 3.25 VLFANGLTTFEE54 pKa = 3.89 QLIVHH59 pKa = 6.61 WSEE62 pKa = 3.22 TDD64 pKa = 2.81 MGYY67 pKa = 10.75 EE68 pKa = 4.14 FLHH71 pKa = 6.74 DD72 pKa = 5.3 LYY74 pKa = 11.73 NSGQKK79 pKa = 8.99 LTVLVPTNDD88 pKa = 3.19 AFQEE92 pKa = 4.19 SGIVSPFEE100 pKa = 4.18 GLTEE104 pKa = 4.05 DD105 pKa = 3.22 WGAEE109 pKa = 3.88 LGEE112 pKa = 4.01 LHH114 pKa = 7.25 LLQGEE119 pKa = 4.25 WTYY122 pKa = 11.83 DD123 pKa = 3.34 QIPQSGHH130 pKa = 5.49 AVAATSLLLANEE142 pKa = 4.47 LNSTDD147 pKa = 3.58 IQPNAYY153 pKa = 9.27 QAMVLEE159 pKa = 4.82 RR160 pKa = 11.84 GNDD163 pKa = 3.42 DD164 pKa = 3.61 SVVVNGWWGHH174 pKa = 4.69 ATSTSGPLDD183 pKa = 3.59 LSGSGGLLDD192 pKa = 4.75 NLFILPIDD200 pKa = 3.73 QVLSFPPSLSTALQAPGLTNMSSALQVIGKK230 pKa = 9.76 SSDD233 pKa = 3.43 VEE235 pKa = 4.1 RR236 pKa = 11.84 LSEE239 pKa = 4.11 GGFTIFVPLDD249 pKa = 3.72 SVWNDD254 pKa = 3.21 EE255 pKa = 5.0 LKK257 pKa = 10.88 DD258 pKa = 3.57 IMLDD262 pKa = 3.49 GSKK265 pKa = 10.54 APEE268 pKa = 3.87 MIGNHH273 pKa = 5.15 FTTSYY278 pKa = 11.56 SLFSPMWIQPSTFEE292 pKa = 4.55 LPVEE296 pKa = 4.5 SGEE299 pKa = 4.65 SLTIQYY305 pKa = 9.53 NQDD308 pKa = 2.93 GSSSVICGEE317 pKa = 3.97 VEE319 pKa = 3.91 AQIVRR324 pKa = 11.84 SDD326 pKa = 2.88 ITLNNGVMHH335 pKa = 7.53 IIDD338 pKa = 4.1 QILYY342 pKa = 6.48 PTSTSSSSDD351 pKa = 3.13 SAKK354 pKa = 10.64 SNKK357 pKa = 7.87 PTTTSSIPSTADD369 pKa = 2.95 STSASIPDD377 pKa = 3.92 GSDD380 pKa = 2.99 SDD382 pKa = 4.24 GQGGKK387 pKa = 9.87 AAIPDD392 pKa = 3.84 APQNSSTSSTVTLSVTLLGCLAVGWLVVYY421 pKa = 8.3 VQQ423 pKa = 3.77
Molecular weight: 45.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.567
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.478
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.706
Sillero 3.91
Patrickios 1.227
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A1B9IT04|A0A1B9IT04_9TREE Uncharacterized protein OS=Kwoniella mangroviensis CBS 10435 OX=1331196 GN=L486_03119 PE=3 SV=1
MM1 pKa = 7.16 AQSEE5 pKa = 4.85 VTQSLGLAIAQTEE18 pKa = 4.28 NLFHH22 pKa = 6.68 KK23 pKa = 10.59 QADD26 pKa = 4.17 LEE28 pKa = 4.3 NSLNRR33 pKa = 11.84 SLAISEE39 pKa = 4.48 RR40 pKa = 11.84 SLKK43 pKa = 10.24 LANRR47 pKa = 11.84 WVSSPSLSLFSGPSSMTLSSLGGVSTQLLIWSLQLIWQIAYY88 pKa = 10.77 SMVSAFCFVFILFRR102 pKa = 11.84 TGMRR106 pKa = 11.84 KK107 pKa = 9.86 SMLRR111 pKa = 11.84 WSNRR115 pKa = 11.84 FYY117 pKa = 10.69 HH118 pKa = 6.35 IPSDD122 pKa = 3.72 EE123 pKa = 4.11 EE124 pKa = 4.22 SAVTSRR130 pKa = 11.84 AKK132 pKa = 10.76 DD133 pKa = 3.41 FTNITISGITQSPSRR148 pKa = 11.84 NRR150 pKa = 11.84 QSCNPTANLAPSPEE164 pKa = 4.35 VTRR167 pKa = 11.84 TPDD170 pKa = 3.1 PRR172 pKa = 11.84 RR173 pKa = 11.84 SNFRR177 pKa = 11.84 RR178 pKa = 11.84 SASAPLL184 pKa = 3.51
Molecular weight: 20.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.516
IPC_protein 10.467
Toseland 10.672
ProMoST 10.452
Dawson 10.76
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.818
Grimsley 10.804
Solomon 10.935
Lehninger 10.891
Nozaki 10.672
DTASelect 10.496
Thurlkill 10.672
EMBOSS 11.082
Sillero 10.701
Patrickios 10.599
IPC_peptide 10.935
IPC2_peptide 9.721
IPC2.peptide.svr19 8.61
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8428
0
8428
4204977
50
4993
498.9
55.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.228 ± 0.029
0.972 ± 0.01
5.649 ± 0.018
6.465 ± 0.03
3.31 ± 0.015
7.093 ± 0.025
2.306 ± 0.011
5.263 ± 0.018
5.402 ± 0.024
8.765 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.052 ± 0.009
3.948 ± 0.016
6.554 ± 0.033
3.969 ± 0.021
5.682 ± 0.021
9.63 ± 0.039
6.104 ± 0.018
5.625 ± 0.019
1.344 ± 0.01
2.639 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here