Colletotrichum chlorophyti
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10302 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q8RSF6|A0A1Q8RSF6_9PEZI AP-2 complex subunit alpha OS=Colletotrichum chlorophyti OX=708187 GN=CCHL11_03660 PE=3 SV=1
MM1 pKa = 7.63 RR2 pKa = 11.84 LIVLAFLTLSTPLISAQDD20 pKa = 3.56 VATTITVGPPSPTSEE35 pKa = 4.47 PEE37 pKa = 3.89 WNSDD41 pKa = 3.42 EE42 pKa = 4.5 TFTSAVLNSTNFYY55 pKa = 10.3 RR56 pKa = 11.84 GEE58 pKa = 3.93 HH59 pKa = 6.09 HH60 pKa = 7.54 ASDD63 pKa = 5.02 LSWNDD68 pKa = 3.31 TLAEE72 pKa = 4.01 FADD75 pKa = 5.13 DD76 pKa = 4.84 YY77 pKa = 11.37 LDD79 pKa = 5.31 DD80 pKa = 4.53 MPGDD84 pKa = 3.84 SCDD87 pKa = 3.88 FSHH90 pKa = 7.29 SGGPYY95 pKa = 10.36 GEE97 pKa = 4.61 NLAKK101 pKa = 10.56 GYY103 pKa = 9.96 PNVTASVEE111 pKa = 3.96 AWGEE115 pKa = 3.78 EE116 pKa = 3.69 RR117 pKa = 11.84 DD118 pKa = 5.3 DD119 pKa = 4.09 YY120 pKa = 12.0 DD121 pKa = 4.18 FDD123 pKa = 4.14 EE124 pKa = 6.09 GEE126 pKa = 3.98 FDD128 pKa = 4.55 ADD130 pKa = 3.54 TGHH133 pKa = 6.24 FTQLVWRR140 pKa = 11.84 NTTDD144 pKa = 3.08 VGCARR149 pKa = 11.84 KK150 pKa = 9.86 LCDD153 pKa = 4.13 DD154 pKa = 3.85 DD155 pKa = 3.0 TWYY158 pKa = 10.02 IVCEE162 pKa = 3.66 YY163 pKa = 9.15 WPRR166 pKa = 11.84 GNVVDD171 pKa = 4.2 QFTDD175 pKa = 3.43 QVQEE179 pKa = 3.82 AFAARR184 pKa = 11.84 TGPKK188 pKa = 9.83 LAFLVAVGVATMWTAAA204 pKa = 3.75
Molecular weight: 22.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.872
IPC_protein 3.884
Toseland 3.656
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.567
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.228
Thurlkill 3.719
EMBOSS 3.821
Sillero 3.999
Patrickios 1.914
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.884
Protein with the highest isoelectric point:
>tr|A0A1Q8RR24|A0A1Q8RR24_9PEZI Baeyer-Villiger monooxygenase 4 OS=Colletotrichum chlorophyti OX=708187 GN=CCHL11_03865 PE=4 SV=1
MM1 pKa = 7.85 PLRR4 pKa = 11.84 STRR7 pKa = 11.84 HH8 pKa = 4.18 TTTTAAPRR16 pKa = 11.84 RR17 pKa = 11.84 SGGLFSRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 AAPATTSRR34 pKa = 11.84 RR35 pKa = 11.84 HH36 pKa = 5.05 HH37 pKa = 6.42 HH38 pKa = 5.62 TTTTTTTTRR47 pKa = 11.84 PKK49 pKa = 10.46 RR50 pKa = 11.84 GGLFSRR56 pKa = 11.84 RR57 pKa = 11.84 RR58 pKa = 11.84 GAVTTAPVHH67 pKa = 4.81 HH68 pKa = 6.22 QRR70 pKa = 11.84 RR71 pKa = 11.84 KK72 pKa = 9.88 PSMSDD77 pKa = 3.22 KK78 pKa = 10.84 ISGAMLKK85 pKa = 10.72 LKK87 pKa = 9.36 GTLTRR92 pKa = 11.84 RR93 pKa = 11.84 PGQKK97 pKa = 9.9 AAGTRR102 pKa = 11.84 RR103 pKa = 11.84 MHH105 pKa = 5.68 GTDD108 pKa = 2.92 GRR110 pKa = 11.84 GSRR113 pKa = 11.84 RR114 pKa = 11.84 AYY116 pKa = 10.23 RR117 pKa = 11.84 YY118 pKa = 9.61
Molecular weight: 13.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.008
IPC_protein 12.427
Toseland 12.588
ProMoST 13.086
Dawson 12.588
Bjellqvist 12.588
Wikipedia 13.056
Rodwell 12.193
Grimsley 12.632
Solomon 13.086
Lehninger 12.983
Nozaki 12.588
DTASelect 12.588
Thurlkill 12.588
EMBOSS 13.086
Sillero 12.588
Patrickios 11.915
IPC_peptide 13.086
IPC2_peptide 12.076
IPC2.peptide.svr19 9.105
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10302
0
10302
5134087
13
11300
498.4
54.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.123 ± 0.021
1.153 ± 0.009
5.73 ± 0.016
6.074 ± 0.021
3.796 ± 0.014
7.142 ± 0.02
2.267 ± 0.01
4.766 ± 0.016
4.864 ± 0.02
8.737 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.155 ± 0.01
3.688 ± 0.014
6.063 ± 0.026
3.865 ± 0.017
6.003 ± 0.018
7.929 ± 0.023
5.991 ± 0.027
6.418 ± 0.018
1.501 ± 0.009
2.734 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here