Cupriavidus sp. OV038

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Cupriavidus; unclassified Cupriavidus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1CYP7|A0A1I1CYP7_9BURK Transcriptional regulator LysR family OS=Cupriavidus sp. OV038 OX=1855313 GN=SAMN05216321_10144 PE=3 SV=1
MM1 pKa = 7.99AYY3 pKa = 9.68QVYY6 pKa = 10.17LPDD9 pKa = 4.85GYY11 pKa = 10.94VQALATPNGGAISGQNVSLDD31 pKa = 2.7IDD33 pKa = 3.81GQLRR37 pKa = 11.84NSGQITAGDD46 pKa = 4.21LLQVKK51 pKa = 9.98AGSLDD56 pKa = 3.55LSPNVVDD63 pKa = 4.92IGTNAYY69 pKa = 8.81KK70 pKa = 10.66AQGGWNVVTGTVVQQ84 pKa = 3.94

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1L9U3|A0A1I1L9U3_9BURK Helicase conserved C-terminal domain-containing protein OS=Cupriavidus sp. OV038 OX=1855313 GN=SAMN05216321_106124 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5334

0

5334

1792796

39

4558

336.1

36.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.149 ± 0.044

0.868 ± 0.01

5.422 ± 0.025

5.011 ± 0.031

3.463 ± 0.021

8.517 ± 0.044

2.246 ± 0.018

4.407 ± 0.023

2.937 ± 0.031

10.266 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.016

2.665 ± 0.022

5.513 ± 0.024

3.732 ± 0.022

7.057 ± 0.038

5.107 ± 0.026

5.542 ± 0.028

7.799 ± 0.03

1.377 ± 0.014

2.377 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski