Amino acid dipepetide frequency for Ralstonia phage RSF1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.506AlaAla: 7.506 ± 0.723
0.684AlaCys: 0.684 ± 0.089
4.965AlaAsp: 4.965 ± 0.339
4.578AlaGlu: 4.578 ± 0.37
2.958AlaPhe: 2.958 ± 0.224
5.648AlaGly: 5.648 ± 0.434
1.397AlaHis: 1.397 ± 0.152
4.504AlaIle: 4.504 ± 0.288
4.311AlaLys: 4.311 ± 0.3
6.243AlaLeu: 6.243 ± 0.361
2.78AlaMet: 2.78 ± 0.191
3.642AlaAsn: 3.642 ± 0.272
2.869AlaPro: 2.869 ± 0.296
3.33AlaGln: 3.33 ± 0.293
3.79AlaArg: 3.79 ± 0.237
4.801AlaSer: 4.801 ± 0.342
4.533AlaThr: 4.533 ± 0.381
5.292AlaVal: 5.292 ± 0.287
0.892AlaTrp: 0.892 ± 0.134
2.824AlaTyr: 2.824 ± 0.231
0.0AlaXaa: 0.0 ± 0.0
Cys
0.55CysAla: 0.55 ± 0.09
0.134CysCys: 0.134 ± 0.043
0.476CysAsp: 0.476 ± 0.087
0.58CysGlu: 0.58 ± 0.102
0.491CysPhe: 0.491 ± 0.084
0.416CysGly: 0.416 ± 0.078
0.342CysHis: 0.342 ± 0.085
0.416CysIle: 0.416 ± 0.079
0.535CysLys: 0.535 ± 0.097
0.699CysLeu: 0.699 ± 0.096
0.208CysMet: 0.208 ± 0.056
0.461CysAsn: 0.461 ± 0.093
0.327CysPro: 0.327 ± 0.076
0.342CysGln: 0.342 ± 0.076
0.505CysArg: 0.505 ± 0.09
0.476CysSer: 0.476 ± 0.077
0.357CysThr: 0.357 ± 0.072
0.505CysVal: 0.505 ± 0.096
0.238CysTrp: 0.238 ± 0.056
0.357CysTyr: 0.357 ± 0.085
0.0CysXaa: 0.0 ± 0.0
Asp
5.396AspAla: 5.396 ± 0.417
0.565AspCys: 0.565 ± 0.089
3.805AspAsp: 3.805 ± 0.316
4.459AspGlu: 4.459 ± 0.293
3.032AspPhe: 3.032 ± 0.212
4.058AspGly: 4.058 ± 0.288
1.516AspHis: 1.516 ± 0.162
3.879AspIle: 3.879 ± 0.278
3.865AspLys: 3.865 ± 0.309
6.347AspLeu: 6.347 ± 0.276
2.2AspMet: 2.2 ± 0.171
2.705AspAsn: 2.705 ± 0.207
3.597AspPro: 3.597 ± 0.296
2.72AspGln: 2.72 ± 0.231
3.82AspArg: 3.82 ± 0.471
3.404AspSer: 3.404 ± 0.222
3.612AspThr: 3.612 ± 0.249
4.102AspVal: 4.102 ± 0.25
0.996AspTrp: 0.996 ± 0.117
2.616AspTyr: 2.616 ± 0.216
0.0AspXaa: 0.0 ± 0.0
Glu
4.935GluAla: 4.935 ± 0.345
0.565GluCys: 0.565 ± 0.098
4.296GluAsp: 4.296 ± 0.334
4.533GluGlu: 4.533 ± 0.281
2.973GluPhe: 2.973 ± 0.259
3.582GluGly: 3.582 ± 0.235
1.367GluHis: 1.367 ± 0.166
4.296GluIle: 4.296 ± 0.308
4.266GluLys: 4.266 ± 0.308
5.96GluLeu: 5.96 ± 0.331
2.17GluMet: 2.17 ± 0.202
2.973GluAsn: 2.973 ± 0.217
2.126GluPro: 2.126 ± 0.195
2.661GluGln: 2.661 ± 0.214
3.003GluArg: 3.003 ± 0.324
3.211GluSer: 3.211 ± 0.242
3.24GluThr: 3.24 ± 0.218
4.088GluVal: 4.088 ± 0.312
0.936GluTrp: 0.936 ± 0.125
2.735GluTyr: 2.735 ± 0.232
0.0GluXaa: 0.0 ± 0.0
Phe
2.884PheAla: 2.884 ± 0.194
0.401PheCys: 0.401 ± 0.08
3.493PheAsp: 3.493 ± 0.225
2.78PheGlu: 2.78 ± 0.239
1.888PhePhe: 1.888 ± 0.209
3.121PheGly: 3.121 ± 0.218
0.818PheHis: 0.818 ± 0.126
2.438PheIle: 2.438 ± 0.218
2.571PheLys: 2.571 ± 0.214
2.988PheLeu: 2.988 ± 0.245
1.457PheMet: 1.457 ± 0.134
2.304PheAsn: 2.304 ± 0.179
1.694PhePro: 1.694 ± 0.185
1.605PheGln: 1.605 ± 0.166
2.289PheArg: 2.289 ± 0.169
2.601PheSer: 2.601 ± 0.198
2.735PheThr: 2.735 ± 0.221
3.181PheVal: 3.181 ± 0.205
0.505PheTrp: 0.505 ± 0.097
2.036PheTyr: 2.036 ± 0.176
0.0PheXaa: 0.0 ± 0.0
Gly
4.831GlyAla: 4.831 ± 0.539
0.476GlyCys: 0.476 ± 0.085
4.043GlyAsp: 4.043 ± 0.392
3.835GlyGlu: 3.835 ± 0.274
3.151GlyPhe: 3.151 ± 0.192
4.712GlyGly: 4.712 ± 0.444
1.367GlyHis: 1.367 ± 0.154
3.85GlyIle: 3.85 ± 0.238
4.474GlyLys: 4.474 ± 0.362
4.831GlyLeu: 4.831 ± 0.314
1.828GlyMet: 1.828 ± 0.172
3.196GlyAsn: 3.196 ± 0.317
2.036GlyPro: 2.036 ± 0.508
2.794GlyGln: 2.794 ± 0.225
3.092GlyArg: 3.092 ± 0.22
3.984GlySer: 3.984 ± 0.33
4.058GlyThr: 4.058 ± 0.43
4.533GlyVal: 4.533 ± 0.281
0.832GlyTrp: 0.832 ± 0.107
2.735GlyTyr: 2.735 ± 0.204
0.0GlyXaa: 0.0 ± 0.0
His
1.412HisAla: 1.412 ± 0.142
0.268HisCys: 0.268 ± 0.078
1.204HisAsp: 1.204 ± 0.142
1.412HisGlu: 1.412 ± 0.155
1.085HisPhe: 1.085 ± 0.148
1.234HisGly: 1.234 ± 0.123
0.669HisHis: 0.669 ± 0.115
1.412HisIle: 1.412 ± 0.136
0.907HisLys: 0.907 ± 0.124
1.858HisLeu: 1.858 ± 0.18
0.669HisMet: 0.669 ± 0.114
0.788HisAsn: 0.788 ± 0.097
1.367HisPro: 1.367 ± 0.126
0.654HisGln: 0.654 ± 0.095
1.234HisArg: 1.234 ± 0.143
1.278HisSer: 1.278 ± 0.149
0.892HisThr: 0.892 ± 0.116
1.367HisVal: 1.367 ± 0.152
0.372HisTrp: 0.372 ± 0.069
1.04HisTyr: 1.04 ± 0.112
0.0HisXaa: 0.0 ± 0.0
Ile
4.667IleAla: 4.667 ± 0.295
0.565IleCys: 0.565 ± 0.099
4.697IleAsp: 4.697 ± 0.279
4.192IleGlu: 4.192 ± 0.238
2.14IlePhe: 2.14 ± 0.193
3.627IleGly: 3.627 ± 0.209
1.189IleHis: 1.189 ± 0.152
2.988IleIle: 2.988 ± 0.252
3.627IleLys: 3.627 ± 0.289
4.429IleLeu: 4.429 ± 0.263
1.412IleMet: 1.412 ± 0.153
3.151IleAsn: 3.151 ± 0.223
2.884IlePro: 2.884 ± 0.236
2.393IleGln: 2.393 ± 0.195
3.404IleArg: 3.404 ± 0.248
3.448IleSer: 3.448 ± 0.23
3.136IleThr: 3.136 ± 0.198
3.865IleVal: 3.865 ± 0.203
0.639IleTrp: 0.639 ± 0.095
2.111IleTyr: 2.111 ± 0.168
0.0IleXaa: 0.0 ± 0.0
Lys
4.4LysAla: 4.4 ± 0.338
0.357LysCys: 0.357 ± 0.073
4.177LysAsp: 4.177 ± 0.347
4.117LysGlu: 4.117 ± 0.27
2.482LysPhe: 2.482 ± 0.167
3.478LysGly: 3.478 ± 0.372
1.353LysHis: 1.353 ± 0.171
3.538LysIle: 3.538 ± 0.242
4.667LysLys: 4.667 ± 0.439
5.396LysLeu: 5.396 ± 0.304
1.932LysMet: 1.932 ± 0.171
3.582LysAsn: 3.582 ± 0.227
2.705LysPro: 2.705 ± 0.205
2.631LysGln: 2.631 ± 0.183
2.854LysArg: 2.854 ± 0.229
3.404LysSer: 3.404 ± 0.213
3.879LysThr: 3.879 ± 0.282
3.225LysVal: 3.225 ± 0.197
0.669LysTrp: 0.669 ± 0.096
2.215LysTyr: 2.215 ± 0.208
0.0LysXaa: 0.0 ± 0.0
Leu
6.377LeuAla: 6.377 ± 0.368
0.788LeuCys: 0.788 ± 0.116
5.381LeuAsp: 5.381 ± 0.222
5.247LeuGlu: 5.247 ± 0.321
3.33LeuPhe: 3.33 ± 0.216
4.831LeuGly: 4.831 ± 0.259
1.561LeuHis: 1.561 ± 0.168
4.147LeuIle: 4.147 ± 0.285
5.619LeuLys: 5.619 ± 0.292
6.139LeuLeu: 6.139 ± 0.351
2.512LeuMet: 2.512 ± 0.179
4.459LeuAsn: 4.459 ± 0.225
3.984LeuPro: 3.984 ± 0.275
3.225LeuGln: 3.225 ± 0.272
4.459LeuArg: 4.459 ± 0.29
5.827LeuSer: 5.827 ± 0.266
5.336LeuThr: 5.336 ± 0.285
5.054LeuVal: 5.054 ± 0.317
1.026LeuTrp: 1.026 ± 0.142
3.3LeuTyr: 3.3 ± 0.213
0.0LeuXaa: 0.0 ± 0.0
Met
2.378MetAla: 2.378 ± 0.214
0.178MetCys: 0.178 ± 0.055
2.14MetAsp: 2.14 ± 0.16
1.769MetGlu: 1.769 ± 0.17
1.189MetPhe: 1.189 ± 0.153
1.605MetGly: 1.605 ± 0.126
0.639MetHis: 0.639 ± 0.096
1.724MetIle: 1.724 ± 0.166
1.962MetLys: 1.962 ± 0.193
2.527MetLeu: 2.527 ± 0.176
0.951MetMet: 0.951 ± 0.133
1.68MetAsn: 1.68 ± 0.163
1.338MetPro: 1.338 ± 0.129
1.323MetGln: 1.323 ± 0.149
1.873MetArg: 1.873 ± 0.19
2.215MetSer: 2.215 ± 0.177
1.947MetThr: 1.947 ± 0.151
1.873MetVal: 1.873 ± 0.155
0.357MetTrp: 0.357 ± 0.073
1.115MetTyr: 1.115 ± 0.134
0.0MetXaa: 0.0 ± 0.0
Asn
3.954AsnAla: 3.954 ± 0.282
0.357AsnCys: 0.357 ± 0.077
2.586AsnAsp: 2.586 ± 0.174
3.211AsnGlu: 3.211 ± 0.235
1.977AsnPhe: 1.977 ± 0.144
4.296AsnGly: 4.296 ± 0.372
0.966AsnHis: 0.966 ± 0.104
2.809AsnIle: 2.809 ± 0.238
2.705AsnLys: 2.705 ± 0.178
4.311AsnLeu: 4.311 ± 0.269
1.427AsnMet: 1.427 ± 0.134
2.304AsnAsn: 2.304 ± 0.208
2.884AsnPro: 2.884 ± 0.217
2.482AsnGln: 2.482 ± 0.182
2.616AsnArg: 2.616 ± 0.162
2.898AsnSer: 2.898 ± 0.201
2.824AsnThr: 2.824 ± 0.213
3.27AsnVal: 3.27 ± 0.258
0.728AsnTrp: 0.728 ± 0.095
1.635AsnTyr: 1.635 ± 0.147
0.0AsnXaa: 0.0 ± 0.0
Pro
3.567ProAla: 3.567 ± 0.33
0.312ProCys: 0.312 ± 0.07
3.285ProAsp: 3.285 ± 0.216
3.434ProGlu: 3.434 ± 0.243
1.635ProPhe: 1.635 ± 0.144
2.542ProGly: 2.542 ± 0.222
1.011ProHis: 1.011 ± 0.112
2.571ProIle: 2.571 ± 0.24
2.69ProLys: 2.69 ± 0.259
3.315ProLeu: 3.315 ± 0.225
1.1ProMet: 1.1 ± 0.111
2.482ProAsn: 2.482 ± 0.175
1.353ProPro: 1.353 ± 0.166
1.62ProGln: 1.62 ± 0.23
1.486ProArg: 1.486 ± 0.153
2.527ProSer: 2.527 ± 0.196
2.78ProThr: 2.78 ± 0.22
2.72ProVal: 2.72 ± 0.214
0.565ProTrp: 0.565 ± 0.097
1.561ProTyr: 1.561 ± 0.16
0.0ProXaa: 0.0 ± 0.0
Gln
4.102GlnAla: 4.102 ± 0.313
0.253GlnCys: 0.253 ± 0.056
2.2GlnAsp: 2.2 ± 0.177
2.23GlnGlu: 2.23 ± 0.198
1.962GlnPhe: 1.962 ± 0.151
2.869GlnGly: 2.869 ± 0.625
1.04GlnHis: 1.04 ± 0.125
2.69GlnIle: 2.69 ± 0.216
2.14GlnLys: 2.14 ± 0.184
3.671GlnLeu: 3.671 ± 0.232
1.189GlnMet: 1.189 ± 0.139
2.155GlnAsn: 2.155 ± 0.157
1.68GlnPro: 1.68 ± 0.151
2.036GlnGln: 2.036 ± 0.232
2.051GlnArg: 2.051 ± 0.181
2.334GlnSer: 2.334 ± 0.161
2.408GlnThr: 2.408 ± 0.221
2.616GlnVal: 2.616 ± 0.218
0.743GlnTrp: 0.743 ± 0.119
2.111GlnTyr: 2.111 ± 0.154
0.0GlnXaa: 0.0 ± 0.0
Arg
3.27ArgAla: 3.27 ± 0.205
0.55ArgCys: 0.55 ± 0.095
3.954ArgAsp: 3.954 ± 0.451
3.642ArgGlu: 3.642 ± 0.433
2.378ArgPhe: 2.378 ± 0.2
3.003ArgGly: 3.003 ± 0.236
1.145ArgHis: 1.145 ± 0.146
3.225ArgIle: 3.225 ± 0.206
2.839ArgLys: 2.839 ± 0.225
4.311ArgLeu: 4.311 ± 0.229
1.665ArgMet: 1.665 ± 0.154
2.646ArgAsn: 2.646 ± 0.227
1.739ArgPro: 1.739 ± 0.148
2.23ArgGln: 2.23 ± 0.22
3.315ArgArg: 3.315 ± 0.346
2.497ArgSer: 2.497 ± 0.197
2.586ArgThr: 2.586 ± 0.219
3.3ArgVal: 3.3 ± 0.221
0.595ArgTrp: 0.595 ± 0.092
2.126ArgTyr: 2.126 ± 0.197
0.0ArgXaa: 0.0 ± 0.0
Ser
4.489SerAla: 4.489 ± 0.344
0.461SerCys: 0.461 ± 0.095
3.909SerAsp: 3.909 ± 0.235
3.27SerGlu: 3.27 ± 0.226
2.973SerPhe: 2.973 ± 0.215
4.028SerGly: 4.028 ± 0.294
1.293SerHis: 1.293 ± 0.144
4.058SerIle: 4.058 ± 0.216
3.211SerLys: 3.211 ± 0.262
5.143SerLeu: 5.143 ± 0.267
1.992SerMet: 1.992 ± 0.182
2.72SerAsn: 2.72 ± 0.23
1.947SerPro: 1.947 ± 0.178
2.631SerGln: 2.631 ± 0.252
2.839SerArg: 2.839 ± 0.206
3.686SerSer: 3.686 ± 0.277
3.092SerThr: 3.092 ± 0.215
3.79SerVal: 3.79 ± 0.247
0.565SerTrp: 0.565 ± 0.081
2.14SerTyr: 2.14 ± 0.163
0.0SerXaa: 0.0 ± 0.0
Thr
4.37ThrAla: 4.37 ± 0.345
0.461ThrCys: 0.461 ± 0.096
3.805ThrAsp: 3.805 ± 0.265
3.255ThrGlu: 3.255 ± 0.237
2.705ThrPhe: 2.705 ± 0.256
4.162ThrGly: 4.162 ± 0.489
1.04ThrHis: 1.04 ± 0.128
3.538ThrIle: 3.538 ± 0.206
3.359ThrLys: 3.359 ± 0.254
4.667ThrLeu: 4.667 ± 0.314
1.65ThrMet: 1.65 ± 0.142
3.092ThrAsn: 3.092 ± 0.277
2.765ThrPro: 2.765 ± 0.197
2.453ThrGln: 2.453 ± 0.243
2.571ThrArg: 2.571 ± 0.197
3.567ThrSer: 3.567 ± 0.27
4.043ThrThr: 4.043 ± 0.303
4.073ThrVal: 4.073 ± 0.249
0.713ThrTrp: 0.713 ± 0.127
2.274ThrTyr: 2.274 ± 0.202
0.0ThrXaa: 0.0 ± 0.0
Val
4.846ValAla: 4.846 ± 0.378
0.505ValCys: 0.505 ± 0.08
4.548ValAsp: 4.548 ± 0.259
4.281ValGlu: 4.281 ± 0.266
2.765ValPhe: 2.765 ± 0.213
4.325ValGly: 4.325 ± 0.455
1.293ValHis: 1.293 ± 0.142
3.627ValIle: 3.627 ± 0.255
4.563ValLys: 4.563 ± 0.229
5.009ValLeu: 5.009 ± 0.241
1.888ValMet: 1.888 ± 0.179
3.389ValAsn: 3.389 ± 0.234
2.839ValPro: 2.839 ± 0.226
2.943ValGln: 2.943 ± 0.198
3.032ValArg: 3.032 ± 0.244
3.404ValSer: 3.404 ± 0.207
4.102ValThr: 4.102 ± 0.285
4.504ValVal: 4.504 ± 0.288
0.743ValTrp: 0.743 ± 0.107
2.542ValTyr: 2.542 ± 0.184
0.0ValXaa: 0.0 ± 0.0
Trp
0.773TrpAla: 0.773 ± 0.107
0.178TrpCys: 0.178 ± 0.046
0.847TrpAsp: 0.847 ± 0.118
0.699TrpGlu: 0.699 ± 0.1
0.684TrpPhe: 0.684 ± 0.123
0.505TrpGly: 0.505 ± 0.08
0.268TrpHis: 0.268 ± 0.069
0.788TrpIle: 0.788 ± 0.114
0.758TrpLys: 0.758 ± 0.088
1.501TrpLeu: 1.501 ± 0.191
0.386TrpMet: 0.386 ± 0.071
0.58TrpAsn: 0.58 ± 0.095
0.416TrpPro: 0.416 ± 0.076
0.639TrpGln: 0.639 ± 0.132
0.639TrpArg: 0.639 ± 0.093
0.699TrpSer: 0.699 ± 0.098
0.654TrpThr: 0.654 ± 0.094
1.04TrpVal: 1.04 ± 0.154
0.134TrpTrp: 0.134 ± 0.041
0.654TrpTyr: 0.654 ± 0.096
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.839TyrAla: 2.839 ± 0.191
0.357TyrCys: 0.357 ± 0.069
2.884TyrAsp: 2.884 ± 0.278
2.393TyrGlu: 2.393 ± 0.188
1.992TyrPhe: 1.992 ± 0.167
2.631TyrGly: 2.631 ± 0.229
0.847TyrHis: 0.847 ± 0.109
2.066TyrIle: 2.066 ± 0.188
1.992TyrLys: 1.992 ± 0.173
3.255TyrLeu: 3.255 ± 0.242
1.353TyrMet: 1.353 ± 0.163
1.932TyrAsn: 1.932 ± 0.182
2.007TyrPro: 2.007 ± 0.186
1.769TyrGln: 1.769 ± 0.194
2.14TyrArg: 2.14 ± 0.172
1.977TyrSer: 1.977 ± 0.165
2.348TyrThr: 2.348 ± 0.234
2.75TyrVal: 2.75 ± 0.197
0.595TyrTrp: 0.595 ± 0.094
1.65TyrTyr: 1.65 ± 0.182
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 237 proteins (67278 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski