Amino acid dipepetide frequency for Alphaproteobacteria bacterium PA3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.866AlaAla: 16.866 ± 0.173	1.259AlaCys: 1.259 ± 0.031	6.689AlaAsp: 6.689 ± 0.07	6.801AlaGlu: 6.801 ± 0.087	4.701AlaPhe: 4.701 ± 0.065	10.462AlaGly: 10.462 ± 0.114	2.332AlaHis: 2.332 ± 0.048	6.491AlaIle: 6.491 ± 0.079	5.004AlaLys: 5.004 ± 0.08	13.825AlaLeu: 13.825 ± 0.131	3.425AlaMet: 3.425 ± 0.056	3.589AlaAsn: 3.589 ± 0.057	6.094AlaPro: 6.094 ± 0.079	4.748AlaGln: 4.748 ± 0.074	8.479AlaArg: 8.479 ± 0.095	7.107AlaSer: 7.107 ± 0.085	6.613AlaThr: 6.613 ± 0.077	7.86AlaVal: 7.86 ± 0.093	1.798AlaTrp: 1.798 ± 0.044	2.657AlaTyr: 2.657 ± 0.048	0.001AlaXaa: 0.001 ± 0.001
Cys	1.062CysAla: 1.062 ± 0.031	0.095CysCys: 0.095 ± 0.009	0.533CysAsp: 0.533 ± 0.019	0.46CysGlu: 0.46 ± 0.019	0.345CysPhe: 0.345 ± 0.015	0.854CysGly: 0.854 ± 0.026	0.208CysHis: 0.208 ± 0.013	0.374CysIle: 0.374 ± 0.018	0.212CysLys: 0.212 ± 0.012	0.918CysLeu: 0.918 ± 0.027	0.142CysMet: 0.142 ± 0.011	0.213CysAsn: 0.213 ± 0.014	0.428CysPro: 0.428 ± 0.021	0.283CysGln: 0.283 ± 0.015	0.526CysArg: 0.526 ± 0.022	0.49CysSer: 0.49 ± 0.021	0.411CysThr: 0.411 ± 0.02	0.673CysVal: 0.673 ± 0.025	0.11CysTrp: 0.11 ± 0.01	0.192CysTyr: 0.192 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.706AspAla: 6.706 ± 0.088	0.456AspCys: 0.456 ± 0.019	2.923AspAsp: 2.923 ± 0.06	3.079AspGlu: 3.079 ± 0.052	2.249AspPhe: 2.249 ± 0.044	5.234AspGly: 5.234 ± 0.081	1.189AspHis: 1.189 ± 0.027	2.701AspIle: 2.701 ± 0.05	1.707AspLys: 1.707 ± 0.038	6.204AspLeu: 6.204 ± 0.077	1.288AspMet: 1.288 ± 0.032	1.315AspAsn: 1.315 ± 0.036	3.575AspPro: 3.575 ± 0.057	2.177AspGln: 2.177 ± 0.042	3.901AspArg: 3.901 ± 0.055	2.394AspSer: 2.394 ± 0.044	2.689AspThr: 2.689 ± 0.059	4.149AspVal: 4.149 ± 0.058	1.038AspTrp: 1.038 ± 0.033	1.397AspTyr: 1.397 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	8.021GluAla: 8.021 ± 0.108	0.282GluCys: 0.282 ± 0.014	2.702GluAsp: 2.702 ± 0.046	2.745GluGlu: 2.745 ± 0.054	1.652GluPhe: 1.652 ± 0.035	4.131GluGly: 4.131 ± 0.063	0.98GluHis: 0.98 ± 0.028	3.087GluIle: 3.087 ± 0.056	2.013GluLys: 2.013 ± 0.05	4.978GluLeu: 4.978 ± 0.07	1.323GluMet: 1.323 ± 0.036	1.404GluAsn: 1.404 ± 0.036	2.604GluPro: 2.604 ± 0.047	1.885GluGln: 1.885 ± 0.039	4.245GluArg: 4.245 ± 0.058	2.403GluSer: 2.403 ± 0.046	3.461GluThr: 3.461 ± 0.054	3.77GluVal: 3.77 ± 0.053	0.654GluTrp: 0.654 ± 0.023	0.838GluTyr: 0.838 ± 0.028	0.001GluXaa: 0.001 ± 0.001
Phe	4.788PheAla: 4.788 ± 0.067	0.396PheCys: 0.396 ± 0.018	2.792PheAsp: 2.792 ± 0.051	2.349PheGlu: 2.349 ± 0.047	1.446PhePhe: 1.446 ± 0.04	3.825PheGly: 3.825 ± 0.067	0.689PheHis: 0.689 ± 0.024	1.636PheIle: 1.636 ± 0.04	1.279PheLys: 1.279 ± 0.036	3.311PheLeu: 3.311 ± 0.057	0.836PheMet: 0.836 ± 0.025	1.204PheAsn: 1.204 ± 0.035	1.492PhePro: 1.492 ± 0.034	1.178PheGln: 1.178 ± 0.028	1.974PheArg: 1.974 ± 0.039	2.338PheSer: 2.338 ± 0.044	2.018PheThr: 2.018 ± 0.047	2.795PheVal: 2.795 ± 0.051	0.571PheTrp: 0.571 ± 0.023	0.972PheTyr: 0.972 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	9.352GlyAla: 9.352 ± 0.118	0.822GlyCys: 0.822 ± 0.027	4.461GlyAsp: 4.461 ± 0.086	4.289GlyGlu: 4.289 ± 0.066	3.801GlyPhe: 3.801 ± 0.062	7.574GlyGly: 7.574 ± 0.147	1.683GlyHis: 1.683 ± 0.035	4.229GlyIle: 4.229 ± 0.06	3.379GlyLys: 3.379 ± 0.061	9.465GlyLeu: 9.465 ± 0.092	2.107GlyMet: 2.107 ± 0.039	2.358GlyAsn: 2.358 ± 0.078	4.014GlyPro: 4.014 ± 0.064	3.444GlyGln: 3.444 ± 0.061	5.57GlyArg: 5.57 ± 0.074	4.907GlySer: 4.907 ± 0.075	4.551GlyThr: 4.551 ± 0.078	6.217GlyVal: 6.217 ± 0.075	1.392GlyTrp: 1.392 ± 0.033	2.119GlyTyr: 2.119 ± 0.04	0.001GlyXaa: 0.001 ± 0.001
His	2.22HisAla: 2.22 ± 0.049	0.218HisCys: 0.218 ± 0.012	1.038HisAsp: 1.038 ± 0.03	1.019HisGlu: 1.019 ± 0.028	0.806HisPhe: 0.806 ± 0.027	1.703HisGly: 1.703 ± 0.038	0.494HisHis: 0.494 ± 0.022	0.854HisIle: 0.854 ± 0.027	0.545HisLys: 0.545 ± 0.019	1.925HisLeu: 1.925 ± 0.04	0.455HisMet: 0.455 ± 0.02	0.468HisAsn: 0.468 ± 0.018	1.161HisPro: 1.161 ± 0.028	0.658HisGln: 0.658 ± 0.024	1.244HisArg: 1.244 ± 0.037	0.91HisSer: 0.91 ± 0.026	0.75HisThr: 0.75 ± 0.025	1.425HisVal: 1.425 ± 0.033	0.358HisTrp: 0.358 ± 0.018	0.536HisTyr: 0.536 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.059IleAla: 7.059 ± 0.071	0.505IleCys: 0.505 ± 0.019	3.404IleAsp: 3.404 ± 0.055	3.386IleGlu: 3.386 ± 0.053	1.649IlePhe: 1.649 ± 0.038	4.933IleGly: 4.933 ± 0.072	0.876IleHis: 0.876 ± 0.027	2.246IleIle: 2.246 ± 0.046	1.649IleLys: 1.649 ± 0.033	4.609IleLeu: 4.609 ± 0.064	0.98IleMet: 0.98 ± 0.028	1.476IleAsn: 1.476 ± 0.034	2.207IlePro: 2.207 ± 0.046	1.469IleGln: 1.469 ± 0.035	2.852IleArg: 2.852 ± 0.053	3.092IleSer: 3.092 ± 0.057	2.697IleThr: 2.697 ± 0.049	3.852IleVal: 3.852 ± 0.06	0.663IleTrp: 0.663 ± 0.024	1.084IleTyr: 1.084 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.985LysAla: 4.985 ± 0.065	0.167LysCys: 0.167 ± 0.012	1.949LysAsp: 1.949 ± 0.047	1.572LysGlu: 1.572 ± 0.037	0.993LysPhe: 0.993 ± 0.032	2.841LysGly: 2.841 ± 0.051	0.64LysHis: 0.64 ± 0.027	1.873LysIle: 1.873 ± 0.043	1.297LysLys: 1.297 ± 0.04	3.597LysLeu: 3.597 ± 0.057	0.822LysMet: 0.822 ± 0.028	0.985LysAsn: 0.985 ± 0.027	2.368LysPro: 2.368 ± 0.045	1.19LysGln: 1.19 ± 0.029	2.398LysArg: 2.398 ± 0.047	2.203LysSer: 2.203 ± 0.042	2.184LysThr: 2.184 ± 0.048	2.542LysVal: 2.542 ± 0.047	0.422LysTrp: 0.422 ± 0.018	0.531LysTyr: 0.531 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	13.863LeuAla: 13.863 ± 0.12	0.875LeuCys: 0.875 ± 0.03	5.926LeuAsp: 5.926 ± 0.073	5.406LeuGlu: 5.406 ± 0.074	3.629LeuPhe: 3.629 ± 0.058	8.451LeuGly: 8.451 ± 0.097	1.743LeuHis: 1.743 ± 0.04	5.219LeuIle: 5.219 ± 0.073	3.82LeuLys: 3.82 ± 0.058	8.644LeuLeu: 8.644 ± 0.109	2.377LeuMet: 2.377 ± 0.043	2.932LeuAsn: 2.932 ± 0.049	5.354LeuPro: 5.354 ± 0.073	3.06LeuGln: 3.06 ± 0.054	6.112LeuArg: 6.112 ± 0.076	6.831LeuSer: 6.831 ± 0.087	5.794LeuThr: 5.794 ± 0.079	7.41LeuVal: 7.41 ± 0.094	1.263LeuTrp: 1.263 ± 0.038	2.001LeuTyr: 2.001 ± 0.038	0.002LeuXaa: 0.002 ± 0.001
Met	3.099MetAla: 3.099 ± 0.048	0.18MetCys: 0.18 ± 0.012	1.129MetAsp: 1.129 ± 0.033	1.061MetGlu: 1.061 ± 0.029	0.705MetPhe: 0.705 ± 0.027	1.965MetGly: 1.965 ± 0.039	0.402MetHis: 0.402 ± 0.017	1.272MetIle: 1.272 ± 0.033	0.915MetLys: 0.915 ± 0.026	2.13MetLeu: 2.13 ± 0.043	0.648MetMet: 0.648 ± 0.026	0.77MetAsn: 0.77 ± 0.023	1.36MetPro: 1.36 ± 0.03	0.801MetGln: 0.801 ± 0.026	1.753MetArg: 1.753 ± 0.037	1.62MetSer: 1.62 ± 0.033	1.768MetThr: 1.768 ± 0.037	1.643MetVal: 1.643 ± 0.037	0.24MetTrp: 0.24 ± 0.014	0.258MetTyr: 0.258 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.308AsnAla: 3.308 ± 0.058	0.244AsnCys: 0.244 ± 0.015	1.578AsnAsp: 1.578 ± 0.06	1.305AsnGlu: 1.305 ± 0.032	1.136AsnPhe: 1.136 ± 0.03	2.555AsnGly: 2.555 ± 0.051	0.486AsnHis: 0.486 ± 0.02	1.371AsnIle: 1.371 ± 0.035	0.901AsnLys: 0.901 ± 0.03	2.955AsnLeu: 2.955 ± 0.053	0.588AsnMet: 0.588 ± 0.019	0.797AsnAsn: 0.797 ± 0.027	2.054AsnPro: 2.054 ± 0.042	1.137AsnGln: 1.137 ± 0.033	1.834AsnArg: 1.834 ± 0.035	1.559AsnSer: 1.559 ± 0.038	1.419AsnThr: 1.419 ± 0.04	2.018AsnVal: 2.018 ± 0.04	0.541AsnTrp: 0.541 ± 0.022	0.725AsnTyr: 0.725 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	6.316ProAla: 6.316 ± 0.081	0.364ProCys: 0.364 ± 0.018	3.606ProAsp: 3.606 ± 0.059	3.445ProGlu: 3.445 ± 0.056	1.998ProPhe: 1.998 ± 0.04	4.148ProGly: 4.148 ± 0.066	0.943ProHis: 0.943 ± 0.026	2.688ProIle: 2.688 ± 0.047	2.123ProLys: 2.123 ± 0.047	4.717ProLeu: 4.717 ± 0.059	1.237ProMet: 1.237 ± 0.029	1.769ProAsn: 1.769 ± 0.039	2.637ProPro: 2.637 ± 0.061	1.815ProGln: 1.815 ± 0.035	2.806ProArg: 2.806 ± 0.049	3.206ProSer: 3.206 ± 0.052	2.868ProThr: 2.868 ± 0.044	3.954ProVal: 3.954 ± 0.06	0.753ProTrp: 0.753 ± 0.026	1.068ProTyr: 1.068 ± 0.03	0.002ProXaa: 0.002 ± 0.001
Gln	5.247GlnAla: 5.247 ± 0.071	0.221GlnCys: 0.221 ± 0.014	1.77GlnAsp: 1.77 ± 0.038	1.624GlnGlu: 1.624 ± 0.034	1.304GlnPhe: 1.304 ± 0.038	2.726GlnGly: 2.726 ± 0.048	0.64GlnHis: 0.64 ± 0.023	2.048GlnIle: 2.048 ± 0.045	1.267GlnLys: 1.267 ± 0.031	3.168GlnLeu: 3.168 ± 0.052	0.851GlnMet: 0.851 ± 0.03	1.037GlnAsn: 1.037 ± 0.026	1.849GlnPro: 1.849 ± 0.043	1.249GlnGln: 1.249 ± 0.036	2.515GlnArg: 2.515 ± 0.05	2.289GlnSer: 2.289 ± 0.045	2.247GlnThr: 2.247 ± 0.055	2.613GlnVal: 2.613 ± 0.046	0.46GlnTrp: 0.46 ± 0.017	0.555GlnTyr: 0.555 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	7.939ArgAla: 7.939 ± 0.09	0.485ArgCys: 0.485 ± 0.019	3.713ArgAsp: 3.713 ± 0.061	3.495ArgGlu: 3.495 ± 0.053	2.876ArgPhe: 2.876 ± 0.051	4.478ArgGly: 4.478 ± 0.059	1.36ArgHis: 1.36 ± 0.037	3.388ArgIle: 3.388 ± 0.059	2.16ArgLys: 2.16 ± 0.048	7.615ArgLeu: 7.615 ± 0.084	1.631ArgMet: 1.631 ± 0.035	1.744ArgAsn: 1.744 ± 0.04	3.329ArgPro: 3.329 ± 0.05	2.503ArgGln: 2.503 ± 0.048	4.597ArgArg: 4.597 ± 0.079	3.473ArgSer: 3.473 ± 0.053	3.446ArgThr: 3.446 ± 0.058	4.491ArgVal: 4.491 ± 0.062	0.96ArgTrp: 0.96 ± 0.03	1.554ArgTyr: 1.554 ± 0.036	0.002ArgXaa: 0.002 ± 0.001
Ser	6.847SerAla: 6.847 ± 0.087	0.487SerCys: 0.487 ± 0.019	3.181SerAsp: 3.181 ± 0.053	2.914SerGlu: 2.914 ± 0.049	2.445SerPhe: 2.445 ± 0.047	5.674SerGly: 5.674 ± 0.081	1.107SerHis: 1.107 ± 0.029	3.023SerIle: 3.023 ± 0.054	2.009SerLys: 2.009 ± 0.041	5.886SerLeu: 5.886 ± 0.072	1.287SerMet: 1.287 ± 0.029	1.754SerAsn: 1.754 ± 0.037	3.191SerPro: 3.191 ± 0.048	2.162SerGln: 2.162 ± 0.047	3.497SerArg: 3.497 ± 0.052	3.266SerSer: 3.266 ± 0.067	3.013SerThr: 3.013 ± 0.058	4.038SerVal: 4.038 ± 0.059	0.883SerTrp: 0.883 ± 0.029	1.386SerTyr: 1.386 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	6.399ThrAla: 6.399 ± 0.086	0.467ThrCys: 0.467 ± 0.02	2.873ThrAsp: 2.873 ± 0.048	2.463ThrGlu: 2.463 ± 0.049	2.039ThrPhe: 2.039 ± 0.043	5.409ThrGly: 5.409 ± 0.091	1.019ThrHis: 1.019 ± 0.028	3.008ThrIle: 3.008 ± 0.051	1.921ThrLys: 1.921 ± 0.041	6.103ThrLeu: 6.103 ± 0.072	1.251ThrMet: 1.251 ± 0.031	1.655ThrAsn: 1.655 ± 0.039	3.445ThrPro: 3.445 ± 0.058	2.042ThrGln: 2.042 ± 0.044	3.284ThrArg: 3.284 ± 0.058	3.314ThrSer: 3.314 ± 0.054	3.037ThrThr: 3.037 ± 0.063	3.542ThrVal: 3.542 ± 0.063	0.733ThrTrp: 0.733 ± 0.027	1.354ThrTyr: 1.354 ± 0.036	0.002ThrXaa: 0.002 ± 0.001
Val	8.803ValAla: 8.803 ± 0.081	0.64ValCys: 0.64 ± 0.023	3.986ValAsp: 3.986 ± 0.059	4.0ValGlu: 4.0 ± 0.057	2.665ValPhe: 2.665 ± 0.05	5.553ValGly: 5.553 ± 0.074	1.204ValHis: 1.204 ± 0.035	3.711ValIle: 3.711 ± 0.055	2.302ValLys: 2.302 ± 0.044	6.862ValLeu: 6.862 ± 0.088	1.692ValMet: 1.692 ± 0.039	1.993ValAsn: 1.993 ± 0.038	3.612ValPro: 3.612 ± 0.062	2.317ValGln: 2.317 ± 0.044	4.782ValArg: 4.782 ± 0.057	4.471ValSer: 4.471 ± 0.065	4.462ValThr: 4.462 ± 0.069	5.278ValVal: 5.278 ± 0.083	1.015ValTrp: 1.015 ± 0.033	1.443ValTyr: 1.443 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.505TrpAla: 1.505 ± 0.037	0.148TrpCys: 0.148 ± 0.011	0.7TrpAsp: 0.7 ± 0.021	0.601TrpGlu: 0.601 ± 0.022	0.564TrpPhe: 0.564 ± 0.024	1.04TrpGly: 1.04 ± 0.03	0.34TrpHis: 0.34 ± 0.017	0.68TrpIle: 0.68 ± 0.027	0.461TrpLys: 0.461 ± 0.019	1.634TrpLeu: 1.634 ± 0.045	0.376TrpMet: 0.376 ± 0.016	0.455TrpAsn: 0.455 ± 0.019	0.734TrpPro: 0.734 ± 0.024	0.642TrpGln: 0.642 ± 0.025	1.251TrpArg: 1.251 ± 0.033	0.975TrpSer: 0.975 ± 0.031	0.882TrpThr: 0.882 ± 0.031	0.944TrpVal: 0.944 ± 0.034	0.234TrpTrp: 0.234 ± 0.013	0.285TrpTyr: 0.285 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.414TyrAla: 2.414 ± 0.05	0.216TyrCys: 0.216 ± 0.013	1.416TyrAsp: 1.416 ± 0.034	1.165TyrGlu: 1.165 ± 0.034	0.887TyrPhe: 0.887 ± 0.026	2.177TyrGly: 2.177 ± 0.05	0.452TyrHis: 0.452 ± 0.021	0.786TyrIle: 0.786 ± 0.026	0.596TyrLys: 0.596 ± 0.024	2.168TyrLeu: 2.168 ± 0.043	0.404TyrMet: 0.404 ± 0.019	0.592TyrAsn: 0.592 ± 0.021	0.956TyrPro: 0.956 ± 0.028	0.923TyrGln: 0.923 ± 0.03	1.618TyrArg: 1.618 ± 0.036	1.199TyrSer: 1.199 ± 0.032	1.048TyrThr: 1.048 ± 0.028	1.574TyrVal: 1.574 ± 0.042	0.364TyrTrp: 0.364 ± 0.015	0.502TyrTyr: 0.502 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.002XaaAsp: 0.002 ± 0.002	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.002XaaHis: 0.002 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.002XaaSer: 0.002 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.018XaaXaa: 0.018 ± 0.005

Statistics based on 4749 proteins (1247484 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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