Serratia phage Scapp

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345L6N8|A0A345L6N8_9CAUD Uncharacterized protein OS=Serratia phage Scapp OX=2282409 GN=CPT_Scapp_011 PE=4 SV=1
MM1 pKa = 6.88QQQFMLIRR9 pKa = 11.84FAGAFGLVPVEE20 pKa = 4.39NFDD23 pKa = 4.14SEE25 pKa = 4.73TGKK28 pKa = 8.67FTPDD32 pKa = 2.78VKK34 pKa = 11.07QPFAPVGYY42 pKa = 9.93SGEE45 pKa = 4.16QQPGAPYY52 pKa = 9.93QHH54 pKa = 6.65TEE56 pKa = 3.96FGQIWVSPLNILNPEE71 pKa = 4.0TAEE74 pKa = 4.13TLVSIAEE81 pKa = 3.78GHH83 pKa = 5.29YY84 pKa = 10.81AEE86 pKa = 5.29NGVEE90 pKa = 4.2EE91 pKa = 5.54PIPAPEE97 pKa = 4.21EE98 pKa = 4.0EE99 pKa = 4.46TDD101 pKa = 3.8GSDD104 pKa = 4.49SSEE107 pKa = 3.88THH109 pKa = 5.98

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345L6N6|A0A345L6N6_9CAUD Uncharacterized protein OS=Serratia phage Scapp OX=2282409 GN=CPT_Scapp_009 PE=4 SV=1
MM1 pKa = 7.93ASRR4 pKa = 11.84ICSSGVLLCRR14 pKa = 11.84RR15 pKa = 11.84PDD17 pKa = 3.49DD18 pKa = 3.64NVRR21 pKa = 11.84FTARR25 pKa = 11.84NPSPVLPRR33 pKa = 11.84EE34 pKa = 4.34DD35 pKa = 5.61DD36 pKa = 3.49PMKK39 pKa = 10.66PKK41 pKa = 10.08FRR43 pKa = 11.84YY44 pKa = 9.18ARR46 pKa = 11.84PAQVSSGMVVSVPYY60 pKa = 10.25EE61 pKa = 4.05SVAGQVYY68 pKa = 7.78RR69 pKa = 11.84TSRR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 9.95NFTVFYY80 pKa = 10.97VRR82 pKa = 11.84FHH84 pKa = 6.99HH85 pKa = 6.62EE86 pKa = 3.67NFLIRR91 pKa = 11.84SRR93 pKa = 11.84VHH95 pKa = 4.8VRR97 pKa = 11.84NDD99 pKa = 2.85EE100 pKa = 4.14RR101 pKa = 11.84VLARR105 pKa = 11.84VPSAHH110 pKa = 6.79AEE112 pKa = 4.08SGFF115 pKa = 3.44

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13498

41

1203

228.8

25.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.35 ± 0.461

1.2 ± 0.167

6.268 ± 0.289

5.86 ± 0.368

3.771 ± 0.214

7.586 ± 0.349

1.593 ± 0.172

5.156 ± 0.169

5.971 ± 0.437

7.246 ± 0.347

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.867 ± 0.203

4.571 ± 0.282

4.297 ± 0.26

3.897 ± 0.278

6.179 ± 0.401

5.216 ± 0.232

5.875 ± 0.45

7.379 ± 0.349

1.63 ± 0.147

3.089 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski