Lactococcus phage bIL286

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9AZS7|Q9AZS7_9CAUD Capsid protein OS=Lactococcus phage bIL286 OX=151536 GN=orf45 PE=4 SV=1
MM1 pKa = 7.36SVTVDD6 pKa = 4.12DD7 pKa = 6.04LLDD10 pKa = 3.62QLSEE14 pKa = 4.54DD15 pKa = 5.09DD16 pKa = 4.43DD17 pKa = 4.84RR18 pKa = 11.84KK19 pKa = 10.14PQLQIYY25 pKa = 9.45FDD27 pKa = 3.58TATAYY32 pKa = 10.31VKK34 pKa = 10.74NAVSSDD40 pKa = 3.45TVDD43 pKa = 3.35APFFNVEE50 pKa = 4.24NVSPIYY56 pKa = 10.5DD57 pKa = 3.71VAVLSYY63 pKa = 11.85SMDD66 pKa = 2.86LWINRR71 pKa = 11.84STTMPPTTAVDD82 pKa = 3.39HH83 pKa = 6.19MVGQLRR89 pKa = 11.84GLYY92 pKa = 10.13SSWKK96 pKa = 9.14EE97 pKa = 3.89AQDD100 pKa = 3.7GQNLQTEE107 pKa = 4.54

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9AZT4|Q9AZT4_9CAUD Orf38 OS=Lactococcus phage bIL286 OX=151536 GN=orf38 PE=4 SV=1
MM1 pKa = 7.78PMTGRR6 pKa = 11.84CRR8 pKa = 11.84EE9 pKa = 4.3PNCHH13 pKa = 5.76AVVVRR18 pKa = 11.84PLHH21 pKa = 5.89YY22 pKa = 9.1CTKK25 pKa = 10.35HH26 pKa = 5.78ADD28 pKa = 3.25KK29 pKa = 10.53EE30 pKa = 4.12AAYY33 pKa = 9.83QASRR37 pKa = 11.84EE38 pKa = 3.86RR39 pKa = 11.84WTNRR43 pKa = 11.84TSNSKK48 pKa = 10.24RR49 pKa = 11.84YY50 pKa = 9.75KK51 pKa = 10.54DD52 pKa = 3.78YY53 pKa = 11.57DD54 pKa = 3.6RR55 pKa = 11.84MRR57 pKa = 11.84SKK59 pKa = 11.37DD60 pKa = 3.44PFKK63 pKa = 11.09AEE65 pKa = 3.5QHH67 pKa = 5.96KK68 pKa = 10.22FYY70 pKa = 10.55QGKK73 pKa = 6.13QWRR76 pKa = 11.84SIRR79 pKa = 11.84EE80 pKa = 3.36IALRR84 pKa = 11.84RR85 pKa = 11.84DD86 pKa = 3.26NYY88 pKa = 10.76LCQYY92 pKa = 9.32CLNHH96 pKa = 6.03KK97 pKa = 9.82RR98 pKa = 11.84VRR100 pKa = 11.84TGNIGDD106 pKa = 4.58HH107 pKa = 5.38IVPYY111 pKa = 10.2EE112 pKa = 4.0VEE114 pKa = 4.06PEE116 pKa = 3.87NRR118 pKa = 11.84TNLANIAIACSKK130 pKa = 10.52CHH132 pKa = 5.24TAKK135 pKa = 10.26TKK137 pKa = 9.95WEE139 pKa = 3.68QLYY142 pKa = 10.74YY143 pKa = 9.01GTGMGNKK150 pKa = 9.12LKK152 pKa = 10.79NAIPIRR158 pKa = 11.84NVKK161 pKa = 9.99DD162 pKa = 3.64LPDD165 pKa = 3.66FQKK168 pKa = 11.01NIRR171 pKa = 3.78

Molecular weight: 20.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

12983

32

1640

212.8

23.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.233 ± 0.583

0.547 ± 0.122

6.216 ± 0.267

6.401 ± 0.424

3.874 ± 0.276

6.555 ± 0.538

1.363 ± 0.161

6.878 ± 0.367

8.288 ± 0.567

7.733 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.192

6.77 ± 0.407

2.704 ± 0.228

4.49 ± 0.37

3.189 ± 0.302

7.302 ± 0.342

7.032 ± 0.711

5.8 ± 0.22

1.333 ± 0.161

3.697 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski