Colletotrichum incanum
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12795 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A166LXT2|A0A166LXT2_9PEZI Uncharacterized protein OS=Colletotrichum incanum OX=1573173 GN=CI238_04498 PE=4 SV=1
MM1 pKa = 7.22 QPPISINYY9 pKa = 8.69 QPALDD14 pKa = 3.68 ATTIQLSASLGQSDD28 pKa = 4.77 ALDD31 pKa = 3.62 SAILNLGLPYY41 pKa = 10.21 VANSGEE47 pKa = 3.83 LSIRR51 pKa = 11.84 VDD53 pKa = 4.03 FNRR56 pKa = 11.84 LFLPCYY62 pKa = 9.66 TIVNLGLEE70 pKa = 4.29 EE71 pKa = 4.75 LNFVLGSSQLKK82 pKa = 10.47 GLLLEE87 pKa = 4.74 AFFDD91 pKa = 4.22 LSEE94 pKa = 4.0
Molecular weight: 10.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.923
IPC_protein 3.77
Toseland 3.592
ProMoST 3.884
Dawson 3.757
Bjellqvist 4.037
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|A0A161VZB4|A0A161VZB4_9PEZI Secreted aspartic proteinase (Fragment) OS=Colletotrichum incanum OX=1573173 GN=CI238_06855 PE=3 SV=1
LL1 pKa = 7.21 VVTPRR6 pKa = 11.84 RR7 pKa = 11.84 VPTSSRR13 pKa = 11.84 PVAPSATPSRR23 pKa = 11.84 RR24 pKa = 11.84 TAATRR29 pKa = 11.84 SALPCTASGAARR41 pKa = 11.84 LAPSRR46 pKa = 11.84 ATPTPTPTSRR56 pKa = 11.84 RR57 pKa = 11.84 ASSGTTRR64 pKa = 11.84 PSLSTSRR71 pKa = 11.84 TPRR74 pKa = 11.84 STSLAPRR81 pKa = 11.84 WPSVVSRR88 pKa = 11.84 RR89 pKa = 11.84 RR90 pKa = 11.84 RR91 pKa = 11.84 TATTSLPTSRR101 pKa = 11.84 TLPNRR106 pKa = 11.84 RR107 pKa = 11.84 IDD109 pKa = 3.41 VV110 pKa = 3.55
Molecular weight: 11.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.46
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.003
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12795
0
12795
6394483
8
12266
499.8
55.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.958 ± 0.019
1.27 ± 0.009
5.605 ± 0.013
5.928 ± 0.02
3.778 ± 0.014
7.011 ± 0.022
2.365 ± 0.011
4.778 ± 0.014
4.79 ± 0.02
8.819 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.158 ± 0.008
3.7 ± 0.012
6.161 ± 0.026
3.936 ± 0.017
6.068 ± 0.019
8.075 ± 0.027
6.085 ± 0.024
6.286 ± 0.017
1.523 ± 0.009
2.705 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here