Torulaspora delbrueckii dsRNA Mbarr-1 killer virus

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 5.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0CG72|A0A0P0CG72_9VIRU Kbarr-1 killer toxin OS=Torulaspora delbrueckii dsRNA Mbarr-1 killer virus OX=1736634 PE=4 SV=1
MM1 pKa = 6.99KK2 pKa = 10.01TNLFMVAAIVFFLRR16 pKa = 11.84LTNVNASQEE25 pKa = 4.14QDD27 pKa = 3.12DD28 pKa = 4.31SAFDD32 pKa = 3.52SRR34 pKa = 11.84IINSVISEE42 pKa = 4.25LRR44 pKa = 11.84GKK46 pKa = 10.07IDD48 pKa = 3.19GCEE51 pKa = 3.84ILVDD55 pKa = 3.85SSGTLVLLPISEE67 pKa = 4.21EE68 pKa = 4.69AEE70 pKa = 4.32FSWEE74 pKa = 4.02DD75 pKa = 3.39SEE77 pKa = 5.13HH78 pKa = 6.43AMSIIDD84 pKa = 4.27VLTKK88 pKa = 10.39YY89 pKa = 10.71NIRR92 pKa = 11.84TEE94 pKa = 4.06ISDD97 pKa = 3.27SRR99 pKa = 11.84AANRR103 pKa = 11.84AGNLGNNVTLLDD115 pKa = 4.48PDD117 pKa = 4.31DD118 pKa = 4.82AFSAGIRR125 pKa = 11.84LVRR128 pKa = 11.84LPHH131 pKa = 5.4VPVRR135 pKa = 11.84QGVTTPAVRR144 pKa = 11.84NNTYY148 pKa = 8.55RR149 pKa = 11.84TRR151 pKa = 11.84STATIEE157 pKa = 4.16ASHH160 pKa = 7.35DD161 pKa = 3.42NADD164 pKa = 3.42NLRR167 pKa = 11.84RR168 pKa = 11.84LSRR171 pKa = 11.84RR172 pKa = 11.84AQWFSPLGVDD182 pKa = 5.34GYY184 pKa = 10.27WDD186 pKa = 3.8KK187 pKa = 11.63NCPGDD192 pKa = 3.72EE193 pKa = 3.99QGYY196 pKa = 8.79SQDD199 pKa = 3.83TADD202 pKa = 4.81PGCSNYY208 pKa = 10.76ASTSYY213 pKa = 10.12MKK215 pKa = 10.01STLTHH220 pKa = 5.44NWCRR224 pKa = 11.84YY225 pKa = 7.74RR226 pKa = 11.84PAGISIWPHH235 pKa = 5.93HH236 pKa = 6.1NCQSGGKK243 pKa = 8.65IHH245 pKa = 6.32TLWAQDD251 pKa = 3.19VAQAVSTRR259 pKa = 11.84TLIHH263 pKa = 6.76GNNQSPMQQ271 pKa = 4.1

Molecular weight: 30.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0CG72|A0A0P0CG72_9VIRU Kbarr-1 killer toxin OS=Torulaspora delbrueckii dsRNA Mbarr-1 killer virus OX=1736634 PE=4 SV=1
MM1 pKa = 6.99KK2 pKa = 10.01TNLFMVAAIVFFLRR16 pKa = 11.84LTNVNASQEE25 pKa = 4.14QDD27 pKa = 3.12DD28 pKa = 4.31SAFDD32 pKa = 3.52SRR34 pKa = 11.84IINSVISEE42 pKa = 4.25LRR44 pKa = 11.84GKK46 pKa = 10.07IDD48 pKa = 3.19GCEE51 pKa = 3.84ILVDD55 pKa = 3.85SSGTLVLLPISEE67 pKa = 4.21EE68 pKa = 4.69AEE70 pKa = 4.32FSWEE74 pKa = 4.02DD75 pKa = 3.39SEE77 pKa = 5.13HH78 pKa = 6.43AMSIIDD84 pKa = 4.27VLTKK88 pKa = 10.39YY89 pKa = 10.71NIRR92 pKa = 11.84TEE94 pKa = 4.06ISDD97 pKa = 3.27SRR99 pKa = 11.84AANRR103 pKa = 11.84AGNLGNNVTLLDD115 pKa = 4.48PDD117 pKa = 4.31DD118 pKa = 4.82AFSAGIRR125 pKa = 11.84LVRR128 pKa = 11.84LPHH131 pKa = 5.4VPVRR135 pKa = 11.84QGVTTPAVRR144 pKa = 11.84NNTYY148 pKa = 8.55RR149 pKa = 11.84TRR151 pKa = 11.84STATIEE157 pKa = 4.16ASHH160 pKa = 7.35DD161 pKa = 3.42NADD164 pKa = 3.42NLRR167 pKa = 11.84RR168 pKa = 11.84LSRR171 pKa = 11.84RR172 pKa = 11.84AQWFSPLGVDD182 pKa = 5.34GYY184 pKa = 10.27WDD186 pKa = 3.8KK187 pKa = 11.63NCPGDD192 pKa = 3.72EE193 pKa = 3.99QGYY196 pKa = 8.79SQDD199 pKa = 3.83TADD202 pKa = 4.81PGCSNYY208 pKa = 10.76ASTSYY213 pKa = 10.12MKK215 pKa = 10.01STLTHH220 pKa = 5.44NWCRR224 pKa = 11.84YY225 pKa = 7.74RR226 pKa = 11.84PAGISIWPHH235 pKa = 5.93HH236 pKa = 6.1NCQSGGKK243 pKa = 8.65IHH245 pKa = 6.32TLWAQDD251 pKa = 3.19VAQAVSTRR259 pKa = 11.84TLIHH263 pKa = 6.76GNNQSPMQQ271 pKa = 4.1

Molecular weight: 30.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

271

271

271

271.0

30.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.118 ± 0.0

1.845 ± 0.0

7.011 ± 0.0

4.059 ± 0.0

2.583 ± 0.0

5.904 ± 0.0

2.952 ± 0.0

6.273 ± 0.0

2.214 ± 0.0

7.38 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.845 ± 0.0

7.011 ± 0.0

4.059 ± 0.0

4.059 ± 0.0

7.011 ± 0.0

9.963 ± 0.0

7.011 ± 0.0

5.904 ± 0.0

2.214 ± 0.0

2.583 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski