Pandoravirus salinus
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1430 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S4W5M0|S4W5M0_9VIRU Uncharacterized protein OS=Pandoravirus salinus OX=1349410 GN=psal_cds_1295 PE=4 SV=1
MM1 pKa = 7.82 AWLQQNGYY9 pKa = 8.45 PPAWPLSDD17 pKa = 6.14 DD18 pKa = 4.88 DD19 pKa = 5.12 DD20 pKa = 6.5 CEE22 pKa = 4.02 DD23 pKa = 4.43 TIAHH27 pKa = 6.97 RR28 pKa = 11.84 DD29 pKa = 3.79 DD30 pKa = 4.85 PNDD33 pKa = 4.09 PFDD36 pKa = 4.56 SYY38 pKa = 11.78 CDD40 pKa = 3.79 DD41 pKa = 5.29 AEE43 pKa = 5.4 DD44 pKa = 5.34 GPLDD48 pKa = 4.0 GNASYY53 pKa = 10.81 EE54 pKa = 3.97 WGGCIDD60 pKa = 5.11 DD61 pKa = 4.7 GGFPYY66 pKa = 10.19 EE67 pKa = 4.45 VQDD70 pKa = 3.64 AHH72 pKa = 6.78 PPNAGVPYY80 pKa = 10.46 AIDD83 pKa = 4.47 DD84 pKa = 4.05 DD85 pKa = 4.41 VTVSGEE91 pKa = 3.99 CLVAPEE97 pKa = 4.47 HH98 pKa = 5.85 TPYY101 pKa = 10.99 AYY103 pKa = 10.15 GYY105 pKa = 7.53 TFIEE109 pKa = 4.79 GFMPADD115 pKa = 3.45 SFDD118 pKa = 3.74 ALVRR122 pKa = 11.84 PEE124 pKa = 3.89 RR125 pKa = 11.84 RR126 pKa = 11.84 LVIMVDD132 pKa = 3.83 GTPVALDD139 pKa = 3.28 AAEE142 pKa = 4.06 VEE144 pKa = 4.58 RR145 pKa = 11.84 AGKK148 pKa = 9.92 VGRR151 pKa = 11.84 ALVTPLGSCVLGPVDD166 pKa = 4.17 VEE168 pKa = 4.32 RR169 pKa = 11.84 AHH171 pKa = 6.46 EE172 pKa = 4.22 ADD174 pKa = 3.67 EE175 pKa = 4.55 ALHH178 pKa = 6.8 DD179 pKa = 4.21 SEE181 pKa = 4.05 GLRR184 pKa = 11.84 RR185 pKa = 11.84 FFTTAPVNAAIEE197 pKa = 4.16 TAAPALGASAGAAADD212 pKa = 3.67 VAGGSILSGVLGGLFAALLL231 pKa = 4.05
Molecular weight: 24.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.151
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.91
Patrickios 0.896
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.797
Protein with the highest isoelectric point:
>tr|S4W5A6|S4W5A6_9VIRU Uncharacterized protein OS=Pandoravirus salinus OX=1349410 GN=psal_cds_1212 PE=4 SV=1
MM1 pKa = 7.62 RR2 pKa = 11.84 LEE4 pKa = 4.84 GKK6 pKa = 7.35 QQRR9 pKa = 11.84 KK10 pKa = 6.62 MKK12 pKa = 10.25 RR13 pKa = 11.84 KK14 pKa = 9.02 RR15 pKa = 11.84 KK16 pKa = 9.49 KK17 pKa = 8.24 EE18 pKa = 3.67 RR19 pKa = 11.84 HH20 pKa = 4.41 RR21 pKa = 11.84 QRR23 pKa = 11.84 PRR25 pKa = 11.84 SQGKK29 pKa = 8.88 KK30 pKa = 9.09 AAAKK34 pKa = 10.09 RR35 pKa = 11.84 GGSAARR41 pKa = 11.84 RR42 pKa = 11.84 VRR44 pKa = 11.84 ATRR47 pKa = 11.84 SAFAGRR53 pKa = 11.84 AAKK56 pKa = 8.65 WRR58 pKa = 11.84 HH59 pKa = 5.23 GMASWRR65 pKa = 11.84 ATTRR69 pKa = 11.84 TPAAPP74 pKa = 3.53
Molecular weight: 8.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.432
IPC2_protein 10.935
IPC_protein 12.515
Toseland 12.676
ProMoST 13.173
Dawson 12.676
Bjellqvist 12.676
Wikipedia 13.159
Rodwell 12.398
Grimsley 12.72
Solomon 13.173
Lehninger 13.071
Nozaki 12.676
DTASelect 12.676
Thurlkill 12.676
EMBOSS 13.173
Sillero 12.676
Patrickios 12.12
IPC_peptide 13.173
IPC2_peptide 12.164
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1430
0
1430
549050
50
2342
384.0
41.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.251 ± 0.091
2.738 ± 0.038
7.65 ± 0.064
3.775 ± 0.037
2.313 ± 0.027
7.839 ± 0.065
3.03 ± 0.036
3.147 ± 0.025
2.118 ± 0.04
8.072 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.978 ± 0.02
2.067 ± 0.028
6.269 ± 0.061
2.594 ± 0.039
9.104 ± 0.069
5.843 ± 0.052
5.589 ± 0.053
7.379 ± 0.046
2.134 ± 0.035
2.111 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here