Pandoravirus salinus 
Average proteome isoelectric point is 6.81 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 1430 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|S4W5M0|S4W5M0_9VIRU Uncharacterized protein OS=Pandoravirus salinus OX=1349410 GN=psal_cds_1295 PE=4 SV=1MM1 pKa = 7.82  AWLQQNGYY9 pKa = 8.45  PPAWPLSDD17 pKa = 6.14  DD18 pKa = 4.88  DD19 pKa = 5.12  DD20 pKa = 6.5  CEE22 pKa = 4.02  DD23 pKa = 4.43  TIAHH27 pKa = 6.97  RR28 pKa = 11.84  DD29 pKa = 3.79  DD30 pKa = 4.85  PNDD33 pKa = 4.09  PFDD36 pKa = 4.56  SYY38 pKa = 11.78  CDD40 pKa = 3.79  DD41 pKa = 5.29  AEE43 pKa = 5.4  DD44 pKa = 5.34  GPLDD48 pKa = 4.0  GNASYY53 pKa = 10.81  EE54 pKa = 3.97  WGGCIDD60 pKa = 5.11  DD61 pKa = 4.7  GGFPYY66 pKa = 10.19  EE67 pKa = 4.45  VQDD70 pKa = 3.64  AHH72 pKa = 6.78  PPNAGVPYY80 pKa = 10.46  AIDD83 pKa = 4.47  DD84 pKa = 4.05  DD85 pKa = 4.41  VTVSGEE91 pKa = 3.99  CLVAPEE97 pKa = 4.47  HH98 pKa = 5.85  TPYY101 pKa = 10.99  AYY103 pKa = 10.15  GYY105 pKa = 7.53  TFIEE109 pKa = 4.79  GFMPADD115 pKa = 3.45  SFDD118 pKa = 3.74  ALVRR122 pKa = 11.84  PEE124 pKa = 3.89  RR125 pKa = 11.84  RR126 pKa = 11.84  LVIMVDD132 pKa = 3.83  GTPVALDD139 pKa = 3.28  AAEE142 pKa = 4.06  VEE144 pKa = 4.58  RR145 pKa = 11.84  AGKK148 pKa = 9.92  VGRR151 pKa = 11.84  ALVTPLGSCVLGPVDD166 pKa = 4.17  VEE168 pKa = 4.32  RR169 pKa = 11.84  AHH171 pKa = 6.46  EE172 pKa = 4.22  ADD174 pKa = 3.67  EE175 pKa = 4.55  ALHH178 pKa = 6.8  DD179 pKa = 4.21  SEE181 pKa = 4.05  GLRR184 pKa = 11.84  RR185 pKa = 11.84  FFTTAPVNAAIEE197 pKa = 4.16  TAAPALGASAGAAADD212 pKa = 3.67  VAGGSILSGVLGGLFAALLL231 pKa = 4.05  
 24.21 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.705 
IPC2_protein 3.77 
IPC_protein 3.783 
Toseland    3.567 
ProMoST     3.948 
Dawson      3.783 
Bjellqvist  3.935 
Wikipedia   3.719 
Rodwell     3.605 
Grimsley    3.465 
Solomon     3.77 
Lehninger   3.732 
Nozaki      3.897 
DTASelect   4.151 
Thurlkill   3.617 
EMBOSS      3.732 
Sillero     3.91 
Patrickios  0.896 
IPC_peptide 3.77 
IPC2_peptide  3.884 
IPC2.peptide.svr19  3.797 
 Protein with the highest isoelectric point: 
>tr|S4W5A6|S4W5A6_9VIRU Uncharacterized protein OS=Pandoravirus salinus OX=1349410 GN=psal_cds_1212 PE=4 SV=1MM1 pKa = 7.62  RR2 pKa = 11.84  LEE4 pKa = 4.84  GKK6 pKa = 7.35  QQRR9 pKa = 11.84  KK10 pKa = 6.62  MKK12 pKa = 10.25  RR13 pKa = 11.84  KK14 pKa = 9.02  RR15 pKa = 11.84  KK16 pKa = 9.49  KK17 pKa = 8.24  EE18 pKa = 3.67  RR19 pKa = 11.84  HH20 pKa = 4.41  RR21 pKa = 11.84  QRR23 pKa = 11.84  PRR25 pKa = 11.84  SQGKK29 pKa = 8.88  KK30 pKa = 9.09  AAAKK34 pKa = 10.09  RR35 pKa = 11.84  GGSAARR41 pKa = 11.84  RR42 pKa = 11.84  VRR44 pKa = 11.84  ATRR47 pKa = 11.84  SAFAGRR53 pKa = 11.84  AAKK56 pKa = 8.65  WRR58 pKa = 11.84  HH59 pKa = 5.23  GMASWRR65 pKa = 11.84  ATTRR69 pKa = 11.84  TPAAPP74 pKa = 3.53  
 8.51 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.432 
IPC2_protein 10.935 
IPC_protein 12.515 
Toseland    12.676 
ProMoST     13.173 
Dawson      12.676 
Bjellqvist  12.676 
Wikipedia   13.159 
Rodwell     12.398 
Grimsley    12.72 
Solomon     13.173 
Lehninger   13.071 
Nozaki      12.676 
DTASelect   12.676 
Thurlkill   12.676 
EMBOSS      13.173 
Sillero     12.676 
Patrickios  12.12 
IPC_peptide 13.173 
IPC2_peptide  12.164 
IPC2.peptide.svr19  9.084 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        1430 
0
1430 
549050
50
2342
384.0
41.53
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        14.251 ± 0.091
2.738 ± 0.038
7.65 ± 0.064
3.775 ± 0.037
2.313 ± 0.027
7.839 ± 0.065
3.03 ± 0.036
3.147 ± 0.025
2.118 ± 0.04
8.072 ± 0.058
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.978 ± 0.02
2.067 ± 0.028
6.269 ± 0.061
2.594 ± 0.039
9.104 ± 0.069
5.843 ± 0.052
5.589 ± 0.053
7.379 ± 0.046
2.134 ± 0.035
2.111 ± 0.032
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here