Streptococcus satellite phage Javan547
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZSN0|A0A4D5ZSN0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan547 OX=2558741 GN=JavanS547_0010 PE=4 SV=1
MM1 pKa = 7.82 AKK3 pKa = 7.93 TTEE6 pKa = 4.0 QTKK9 pKa = 8.33 TVQLTVEE16 pKa = 4.37 EE17 pKa = 4.49 LQSLGCRR24 pKa = 11.84 LSNILKK30 pKa = 8.24 TIKK33 pKa = 9.45 MDD35 pKa = 3.49 QVAQAGVSLAKK46 pKa = 10.69 DD47 pKa = 3.09 RR48 pKa = 11.84 DD49 pKa = 4.19 SFTFTHH55 pKa = 7.29 LATSYY60 pKa = 10.64 LSSSYY65 pKa = 10.78 EE66 pKa = 3.85 VFEE69 pKa = 4.45 MIIAEE74 pKa = 4.41 LDD76 pKa = 4.34 DD77 pKa = 4.12 IASQLLEE84 pKa = 4.78 CDD86 pKa = 3.55 DD87 pKa = 4.73 AEE89 pKa = 4.16 EE90 pKa = 5.29 LEE92 pKa = 4.39 GFRR95 pKa = 11.84 NGRR98 pKa = 3.5
Molecular weight: 10.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.538
IPC2_protein 4.482
IPC_protein 4.355
Toseland 4.202
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.177
Rodwell 4.202
Grimsley 4.113
Solomon 4.304
Lehninger 4.253
Nozaki 4.418
DTASelect 4.558
Thurlkill 4.215
EMBOSS 4.19
Sillero 4.469
Patrickios 3.897
IPC_peptide 4.304
IPC2_peptide 4.457
IPC2.peptide.svr19 4.41
Protein with the highest isoelectric point:
>tr|A0A4D5ZVG0|A0A4D5ZVG0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan547 OX=2558741 GN=JavanS547_0011 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.11 IVEE5 pKa = 4.32 VKK7 pKa = 10.58 KK8 pKa = 10.59 KK9 pKa = 10.48 DD10 pKa = 3.25 GSKK13 pKa = 10.06 VYY15 pKa = 10.08 RR16 pKa = 11.84 ASVYY20 pKa = 10.76 LGVDD24 pKa = 2.9 QVTGKK29 pKa = 10.15 KK30 pKa = 10.12 VKK32 pKa = 9.8 TSITGRR38 pKa = 11.84 TRR40 pKa = 11.84 KK41 pKa = 8.72 EE42 pKa = 4.07 VKK44 pKa = 10.28 SKK46 pKa = 10.57 AQHH49 pKa = 5.58 AQFDD53 pKa = 4.38 FKK55 pKa = 11.59 LNGSTVHH62 pKa = 6.3 KK63 pKa = 10.23 AVRR66 pKa = 11.84 VKK68 pKa = 10.68 NYY70 pKa = 10.33 QEE72 pKa = 5.57 LADD75 pKa = 4.72 LWLEE79 pKa = 4.3 SYY81 pKa = 10.85 QLTVKK86 pKa = 9.85 PQTFIATKK94 pKa = 10.17 RR95 pKa = 11.84 MVYY98 pKa = 10.06 SHH100 pKa = 7.65 LIPVFGKK107 pKa = 9.72 MKK109 pKa = 10.13 LDD111 pKa = 3.56 KK112 pKa = 10.75 LAASYY117 pKa = 10.54 IQGFVNDD124 pKa = 4.64 LSSHH128 pKa = 5.97 LVHH131 pKa = 6.81 FGVVHH136 pKa = 6.77 SINSRR141 pKa = 11.84 ILKK144 pKa = 10.27 YY145 pKa = 10.45 GVSLQLIPFNPARR158 pKa = 11.84 DD159 pKa = 3.52 IILPRR164 pKa = 11.84 KK165 pKa = 8.8 PKK167 pKa = 10.42 RR168 pKa = 11.84 EE169 pKa = 3.8 NKK171 pKa = 9.61 SIKK174 pKa = 10.17 FISPEE179 pKa = 3.9 DD180 pKa = 3.73 LKK182 pKa = 10.59 TLLDD186 pKa = 3.9 YY187 pKa = 10.65 MEE189 pKa = 4.9 KK190 pKa = 10.63 LAFKK194 pKa = 10.06 KK195 pKa = 10.39 YY196 pKa = 10.76 SYY198 pKa = 10.84 FFDD201 pKa = 3.47 YY202 pKa = 10.38 MLYY205 pKa = 10.51 SVLLATGCRR214 pKa = 11.84 FGEE217 pKa = 4.29 AVALEE222 pKa = 4.2 WSDD225 pKa = 3.65 IDD227 pKa = 5.39 LEE229 pKa = 4.22 NATISISKK237 pKa = 10.02 NYY239 pKa = 9.17 NRR241 pKa = 11.84 LVDD244 pKa = 4.31 LVGPPKK250 pKa = 10.52 SKK252 pKa = 10.78 AGVRR256 pKa = 11.84 VISIDD261 pKa = 3.3 RR262 pKa = 11.84 KK263 pKa = 7.22 TVNMLKK269 pKa = 10.23 LYY271 pKa = 10.65 KK272 pKa = 10.14 NRR274 pKa = 11.84 QRR276 pKa = 11.84 QLFIEE281 pKa = 4.88 DD282 pKa = 3.56 GARR285 pKa = 11.84 APSVVFATPTKK296 pKa = 10.09 KK297 pKa = 9.84 YY298 pKa = 10.66 QNMATRR304 pKa = 11.84 QDD306 pKa = 3.65 SLDD309 pKa = 3.75 RR310 pKa = 11.84 RR311 pKa = 11.84 CKK313 pKa = 9.49 EE314 pKa = 3.43 AGIPRR319 pKa = 11.84 FTFHH323 pKa = 7.74 AFRR326 pKa = 11.84 HH327 pKa = 4.69 THH329 pKa = 6.92 ASLLLNAGISYY340 pKa = 10.68 KK341 pKa = 10.23 EE342 pKa = 3.61 LQYY345 pKa = 11.49 RR346 pKa = 11.84 LGHH349 pKa = 6.19 ATLAMTMDD357 pKa = 4.24 IYY359 pKa = 11.79 SHH361 pKa = 6.95 LSKK364 pKa = 10.92 DD365 pKa = 3.51 KK366 pKa = 10.41 EE367 pKa = 4.25 KK368 pKa = 10.93 EE369 pKa = 3.85 AVSYY373 pKa = 9.52 YY374 pKa = 10.59 EE375 pKa = 4.03 KK376 pKa = 10.7 AINGLL381 pKa = 3.67
Molecular weight: 43.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.091
IPC2_protein 9.341
IPC_protein 9.253
Toseland 10.028
ProMoST 9.677
Dawson 10.218
Bjellqvist 9.867
Wikipedia 10.365
Rodwell 10.745
Grimsley 10.277
Solomon 10.233
Lehninger 10.189
Nozaki 9.999
DTASelect 9.853
Thurlkill 10.072
EMBOSS 10.423
Sillero 10.116
Patrickios 10.277
IPC_peptide 10.233
IPC2_peptide 8.361
IPC2.peptide.svr19 8.298
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16
0
16
2795
44
491
174.7
19.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.691 ± 0.344
0.644 ± 0.136
4.973 ± 0.369
8.193 ± 0.782
4.258 ± 0.305
4.973 ± 0.378
2.039 ± 0.269
6.44 ± 0.54
9.088 ± 0.719
9.767 ± 0.445
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.397 ± 0.262
5.188 ± 0.585
3.864 ± 0.581
4.365 ± 0.461
4.723 ± 0.464
6.047 ± 0.668
6.297 ± 0.495
5.295 ± 0.544
0.751 ± 0.184
4.007 ± 0.319
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here