Bacillus phage BCJA1c

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Jarrellvirus; Bacillus virus BCAJ1

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5YA53|Q5YA53_9CAUD Holin OS=Bacillus phage BCJA1c OX=294382 GN=57 PE=4 SV=1
MM1 pKa = 7.51SFKK4 pKa = 10.33TEE6 pKa = 4.02CALCGQEE13 pKa = 3.75IPYY16 pKa = 10.21DD17 pKa = 3.87EE18 pKa = 4.54KK19 pKa = 11.41CFMEE23 pKa = 5.06GNHH26 pKa = 6.77GEE28 pKa = 4.27PTCLTKK34 pKa = 10.7EE35 pKa = 3.92EE36 pKa = 4.45CKK38 pKa = 10.81APEE41 pKa = 3.9EE42 pKa = 4.35WEE44 pKa = 4.07ADD46 pKa = 3.73VVSDD50 pKa = 5.66DD51 pKa = 3.66EE52 pKa = 4.78LEE54 pKa = 4.38EE55 pKa = 5.44GGEE58 pKa = 4.02QQ59 pKa = 4.11

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5YA75|Q5YA75_9CAUD Portal OS=Bacillus phage BCJA1c OX=294382 GN=35 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84KK3 pKa = 9.14IKK5 pKa = 10.53LALHH9 pKa = 5.14YY10 pKa = 10.15FKK12 pKa = 10.96LYY14 pKa = 8.75RR15 pKa = 11.84HH16 pKa = 6.24KK17 pKa = 10.81LISPKK22 pKa = 8.53FTRR25 pKa = 11.84KK26 pKa = 10.06KK27 pKa = 9.99IFEE30 pKa = 4.42SLFKK34 pKa = 10.97SGG36 pKa = 4.26

Molecular weight: 4.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12682

36

1445

218.7

24.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.931 ± 0.744

0.686 ± 0.146

6.521 ± 0.231

8.697 ± 0.379

3.698 ± 0.291

6.269 ± 0.255

2.003 ± 0.151

6.245 ± 0.272

7.862 ± 0.597

7.94 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.61 ± 0.206

5.22 ± 0.252

3.201 ± 0.242

4.613 ± 0.246

5.141 ± 0.275

5.78 ± 0.314

5.433 ± 0.328

6.127 ± 0.22

1.254 ± 0.15

3.769 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski