Amino acid dipepetide frequency for Brevibacillus sp. WF146

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.682AlaAla: 10.682 ± 0.13	0.894AlaCys: 0.894 ± 0.026	5.011AlaAsp: 5.011 ± 0.075	6.76AlaGlu: 6.76 ± 0.083	3.706AlaPhe: 3.706 ± 0.058	7.888AlaGly: 7.888 ± 0.107	1.804AlaHis: 1.804 ± 0.038	5.62AlaIle: 5.62 ± 0.073	4.648AlaLys: 4.648 ± 0.073	9.533AlaLeu: 9.533 ± 0.106	2.565AlaMet: 2.565 ± 0.052	2.662AlaAsn: 2.662 ± 0.051	3.047AlaPro: 3.047 ± 0.061	3.091AlaGln: 3.091 ± 0.056	5.069AlaArg: 5.069 ± 0.072	4.629AlaSer: 4.629 ± 0.071	3.694AlaThr: 3.694 ± 0.062	8.117AlaVal: 8.117 ± 0.096	1.011AlaTrp: 1.011 ± 0.03	2.941AlaTyr: 2.941 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.618CysAla: 0.618 ± 0.023	0.126CysCys: 0.126 ± 0.013	0.438CysAsp: 0.438 ± 0.022	0.466CysGlu: 0.466 ± 0.021	0.335CysPhe: 0.335 ± 0.016	0.875CysGly: 0.875 ± 0.029	0.222CysHis: 0.222 ± 0.014	0.442CysIle: 0.442 ± 0.02	0.297CysLys: 0.297 ± 0.018	0.86CysLeu: 0.86 ± 0.028	0.187CysMet: 0.187 ± 0.013	0.224CysAsn: 0.224 ± 0.015	0.383CysPro: 0.383 ± 0.019	0.239CysGln: 0.239 ± 0.014	0.645CysArg: 0.645 ± 0.029	0.513CysSer: 0.513 ± 0.021	0.426CysThr: 0.426 ± 0.019	0.569CysVal: 0.569 ± 0.023	0.09CysTrp: 0.09 ± 0.009	0.247CysTyr: 0.247 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.18AspAla: 4.18 ± 0.059	0.386AspCys: 0.386 ± 0.018	2.437AspAsp: 2.437 ± 0.062	4.138AspGlu: 4.138 ± 0.065	1.904AspPhe: 1.904 ± 0.041	3.97AspGly: 3.97 ± 0.074	1.025AspHis: 1.025 ± 0.031	3.046AspIle: 3.046 ± 0.056	2.479AspLys: 2.479 ± 0.057	4.825AspLeu: 4.825 ± 0.058	1.302AspMet: 1.302 ± 0.031	1.287AspAsn: 1.287 ± 0.033	2.537AspPro: 2.537 ± 0.054	1.689AspGln: 1.689 ± 0.036	3.135AspArg: 3.135 ± 0.058	2.128AspSer: 2.128 ± 0.045	2.221AspThr: 2.221 ± 0.044	4.441AspVal: 4.441 ± 0.07	0.754AspTrp: 0.754 ± 0.024	1.631AspTyr: 1.631 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	7.053GluAla: 7.053 ± 0.088	0.415GluCys: 0.415 ± 0.018	2.86GluAsp: 2.86 ± 0.056	6.096GluGlu: 6.096 ± 0.088	1.842GluPhe: 1.842 ± 0.042	4.238GluGly: 4.238 ± 0.064	1.557GluHis: 1.557 ± 0.035	4.309GluIle: 4.309 ± 0.065	4.396GluLys: 4.396 ± 0.074	7.048GluLeu: 7.048 ± 0.083	2.038GluMet: 2.038 ± 0.043	2.202GluAsn: 2.202 ± 0.048	2.406GluPro: 2.406 ± 0.054	3.77GluGln: 3.77 ± 0.062	5.543GluArg: 5.543 ± 0.087	2.956GluSer: 2.956 ± 0.049	3.773GluThr: 3.773 ± 0.06	4.751GluVal: 4.751 ± 0.073	0.983GluTrp: 0.983 ± 0.035	1.759GluTyr: 1.759 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	3.996PheAla: 3.996 ± 0.067	0.405PheCys: 0.405 ± 0.02	2.111PheAsp: 2.111 ± 0.043	2.067PheGlu: 2.067 ± 0.043	1.94PhePhe: 1.94 ± 0.048	3.228PheGly: 3.228 ± 0.053	0.975PheHis: 0.975 ± 0.031	2.045PheIle: 2.045 ± 0.045	1.198PheLys: 1.198 ± 0.039	4.346PheLeu: 4.346 ± 0.075	0.856PheMet: 0.856 ± 0.028	1.06PheAsn: 1.06 ± 0.03	1.831PhePro: 1.831 ± 0.047	1.397PheGln: 1.397 ± 0.04	2.248PheArg: 2.248 ± 0.044	2.484PheSer: 2.484 ± 0.049	2.203PheThr: 2.203 ± 0.047	3.258PheVal: 3.258 ± 0.062	0.52PheTrp: 0.52 ± 0.022	1.221PheTyr: 1.221 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	6.141GlyAla: 6.141 ± 0.096	0.821GlyCys: 0.821 ± 0.03	3.462GlyAsp: 3.462 ± 0.07	4.984GlyGlu: 4.984 ± 0.066	3.166GlyPhe: 3.166 ± 0.056	5.893GlyGly: 5.893 ± 0.088	1.595GlyHis: 1.595 ± 0.036	5.392GlyIle: 5.392 ± 0.068	4.388GlyLys: 4.388 ± 0.069	7.575GlyLeu: 7.575 ± 0.093	2.476GlyMet: 2.476 ± 0.056	2.076GlyAsn: 2.076 ± 0.051	2.252GlyPro: 2.252 ± 0.052	2.715GlyGln: 2.715 ± 0.054	4.506GlyArg: 4.506 ± 0.07	4.226GlySer: 4.226 ± 0.055	4.641GlyThr: 4.641 ± 0.067	6.366GlyVal: 6.366 ± 0.085	1.072GlyTrp: 1.072 ± 0.033	2.778GlyTyr: 2.778 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	1.871HisAla: 1.871 ± 0.052	0.191HisCys: 0.191 ± 0.014	1.058HisAsp: 1.058 ± 0.032	1.379HisGlu: 1.379 ± 0.035	1.0HisPhe: 1.0 ± 0.03	1.727HisGly: 1.727 ± 0.04	0.633HisHis: 0.633 ± 0.029	1.259HisIle: 1.259 ± 0.031	0.809HisLys: 0.809 ± 0.03	2.402HisLeu: 2.402 ± 0.05	0.54HisMet: 0.54 ± 0.023	0.624HisAsn: 0.624 ± 0.024	1.488HisPro: 1.488 ± 0.044	0.803HisGln: 0.803 ± 0.028	1.281HisArg: 1.281 ± 0.031	1.006HisSer: 1.006 ± 0.028	1.046HisThr: 1.046 ± 0.034	1.935HisVal: 1.935 ± 0.047	0.27HisTrp: 0.27 ± 0.015	0.759HisTyr: 0.759 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.218IleAla: 6.218 ± 0.08	0.617IleCys: 0.617 ± 0.023	3.316IleAsp: 3.316 ± 0.056	3.701IleGlu: 3.701 ± 0.064	1.947IlePhe: 1.947 ± 0.049	5.337IleGly: 5.337 ± 0.078	1.303IleHis: 1.303 ± 0.033	3.098IleIle: 3.098 ± 0.061	2.445IleLys: 2.445 ± 0.048	5.588IleLeu: 5.588 ± 0.085	1.318IleMet: 1.318 ± 0.033	1.927IleAsn: 1.927 ± 0.042	3.185IlePro: 3.185 ± 0.055	1.982IleGln: 1.982 ± 0.05	3.969IleArg: 3.969 ± 0.062	3.259IleSer: 3.259 ± 0.055	3.301IleThr: 3.301 ± 0.057	5.225IleVal: 5.225 ± 0.071	0.542IleTrp: 0.542 ± 0.022	1.594IleTyr: 1.594 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	4.276LysAla: 4.276 ± 0.066	0.226LysCys: 0.226 ± 0.013	2.208LysAsp: 2.208 ± 0.045	4.068LysGlu: 4.068 ± 0.081	1.07LysPhe: 1.07 ± 0.033	3.622LysGly: 3.622 ± 0.06	1.002LysHis: 1.002 ± 0.032	2.502LysIle: 2.502 ± 0.05	3.027LysLys: 3.027 ± 0.065	4.577LysLeu: 4.577 ± 0.064	1.416LysMet: 1.416 ± 0.038	1.633LysAsn: 1.633 ± 0.046	2.345LysPro: 2.345 ± 0.045	2.408LysGln: 2.408 ± 0.05	3.766LysArg: 3.766 ± 0.056	2.174LysSer: 2.174 ± 0.044	2.754LysThr: 2.754 ± 0.051	3.523LysVal: 3.523 ± 0.073	0.745LysTrp: 0.745 ± 0.025	1.296LysTyr: 1.296 ± 0.036	0.0LysXaa: 0.0 ± 0.0
Leu	10.66LeuAla: 10.66 ± 0.1	0.838LeuCys: 0.838 ± 0.032	5.121LeuAsp: 5.121 ± 0.084	6.54LeuGlu: 6.54 ± 0.087	4.687LeuPhe: 4.687 ± 0.088	7.344LeuGly: 7.344 ± 0.095	2.319LeuHis: 2.319 ± 0.051	5.862LeuIle: 5.862 ± 0.089	4.6LeuLys: 4.6 ± 0.077	11.37LeuLeu: 11.37 ± 0.162	2.489LeuMet: 2.489 ± 0.052	3.019LeuAsn: 3.019 ± 0.051	4.917LeuPro: 4.917 ± 0.069	3.944LeuGln: 3.944 ± 0.065	6.024LeuArg: 6.024 ± 0.089	6.09LeuSer: 6.09 ± 0.074	5.634LeuThr: 5.634 ± 0.076	7.555LeuVal: 7.555 ± 0.089	0.986LeuTrp: 0.986 ± 0.032	3.215LeuTyr: 3.215 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.613MetAla: 2.613 ± 0.044	0.154MetCys: 0.154 ± 0.011	1.348MetAsp: 1.348 ± 0.031	1.951MetGlu: 1.951 ± 0.039	0.927MetPhe: 0.927 ± 0.032	1.971MetGly: 1.971 ± 0.051	0.441MetHis: 0.441 ± 0.019	1.661MetIle: 1.661 ± 0.04	1.67MetLys: 1.67 ± 0.042	2.635MetLeu: 2.635 ± 0.052	0.772MetMet: 0.772 ± 0.029	1.131MetAsn: 1.131 ± 0.031	1.157MetPro: 1.157 ± 0.027	0.966MetGln: 0.966 ± 0.03	1.607MetArg: 1.607 ± 0.034	1.535MetSer: 1.535 ± 0.041	1.548MetThr: 1.548 ± 0.038	1.863MetVal: 1.863 ± 0.046	0.243MetTrp: 0.243 ± 0.016	0.685MetTyr: 0.685 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.502AsnAla: 2.502 ± 0.048	0.225AsnCys: 0.225 ± 0.014	1.372AsnAsp: 1.372 ± 0.039	1.921AsnGlu: 1.921 ± 0.045	0.956AsnPhe: 0.956 ± 0.033	2.749AsnGly: 2.749 ± 0.058	0.681AsnHis: 0.681 ± 0.024	1.786AsnIle: 1.786 ± 0.039	1.443AsnLys: 1.443 ± 0.039	2.895AsnLeu: 2.895 ± 0.056	0.791AsnMet: 0.791 ± 0.028	0.975AsnAsn: 0.975 ± 0.028	1.978AsnPro: 1.978 ± 0.041	1.267AsnGln: 1.267 ± 0.032	2.118AsnArg: 2.118 ± 0.042	1.237AsnSer: 1.237 ± 0.034	1.483AsnThr: 1.483 ± 0.031	2.474AsnVal: 2.474 ± 0.05	0.413AsnTrp: 0.413 ± 0.021	0.82AsnTyr: 0.82 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	4.073ProAla: 4.073 ± 0.071	0.275ProCys: 0.275 ± 0.016	2.983ProAsp: 2.983 ± 0.054	3.476ProGlu: 3.476 ± 0.049	2.117ProPhe: 2.117 ± 0.045	3.418ProGly: 3.418 ± 0.063	1.195ProHis: 1.195 ± 0.034	2.478ProIle: 2.478 ± 0.052	1.688ProLys: 1.688 ± 0.038	4.603ProLeu: 4.603 ± 0.075	1.013ProMet: 1.013 ± 0.027	1.371ProAsn: 1.371 ± 0.035	1.685ProPro: 1.685 ± 0.045	1.554ProGln: 1.554 ± 0.04	2.033ProArg: 2.033 ± 0.045	2.265ProSer: 2.265 ± 0.046	2.086ProThr: 2.086 ± 0.047	3.939ProVal: 3.939 ± 0.061	0.452ProTrp: 0.452 ± 0.02	1.651ProTyr: 1.651 ± 0.048	0.0ProXaa: 0.0 ± 0.0
Gln	4.144GlnAla: 4.144 ± 0.068	0.191GlnCys: 0.191 ± 0.014	1.486GlnAsp: 1.486 ± 0.034	2.815GlnGlu: 2.815 ± 0.052	1.427GlnPhe: 1.427 ± 0.037	2.399GlnGly: 2.399 ± 0.046	0.808GlnHis: 0.808 ± 0.025	2.225GlnIle: 2.225 ± 0.044	1.997GlnLys: 1.997 ± 0.042	3.719GlnLeu: 3.719 ± 0.062	1.184GlnMet: 1.184 ± 0.032	1.069GlnAsn: 1.069 ± 0.03	1.907GlnPro: 1.907 ± 0.044	1.987GlnGln: 1.987 ± 0.054	2.327GlnArg: 2.327 ± 0.041	1.855GlnSer: 1.855 ± 0.044	2.191GlnThr: 2.191 ± 0.044	3.025GlnVal: 3.025 ± 0.048	0.515GlnTrp: 0.515 ± 0.023	1.134GlnTyr: 1.134 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	4.52ArgAla: 4.52 ± 0.065	0.473ArgCys: 0.473 ± 0.022	3.116ArgAsp: 3.116 ± 0.053	5.365ArgGlu: 5.365 ± 0.081	2.735ArgPhe: 2.735 ± 0.048	3.584ArgGly: 3.584 ± 0.056	1.534ArgHis: 1.534 ± 0.04	3.946ArgIle: 3.946 ± 0.056	3.097ArgLys: 3.097 ± 0.053	6.975ArgLeu: 6.975 ± 0.104	1.962ArgMet: 1.962 ± 0.042	1.775ArgAsn: 1.775 ± 0.045	2.455ArgPro: 2.455 ± 0.055	2.913ArgGln: 2.913 ± 0.058	3.962ArgArg: 3.962 ± 0.076	3.007ArgSer: 3.007 ± 0.057	3.025ArgThr: 3.025 ± 0.06	4.363ArgVal: 4.363 ± 0.061	0.768ArgTrp: 0.768 ± 0.028	2.211ArgTyr: 2.211 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	4.438SerAla: 4.438 ± 0.062	0.415SerCys: 0.415 ± 0.022	2.375SerAsp: 2.375 ± 0.05	2.954SerGlu: 2.954 ± 0.059	2.552SerPhe: 2.552 ± 0.055	4.702SerGly: 4.702 ± 0.057	1.161SerHis: 1.161 ± 0.033	3.278SerIle: 3.278 ± 0.05	2.098SerLys: 2.098 ± 0.046	5.798SerLeu: 5.798 ± 0.074	1.479SerMet: 1.479 ± 0.034	1.294SerAsn: 1.294 ± 0.034	2.502SerPro: 2.502 ± 0.057	1.707SerGln: 1.707 ± 0.041	3.284SerArg: 3.284 ± 0.057	2.802SerSer: 2.802 ± 0.052	2.42SerThr: 2.42 ± 0.043	4.159SerVal: 4.159 ± 0.062	0.629SerTrp: 0.629 ± 0.026	1.748SerTyr: 1.748 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	5.012ThrAla: 5.012 ± 0.066	0.446ThrCys: 0.446 ± 0.02	2.481ThrAsp: 2.481 ± 0.048	3.117ThrGlu: 3.117 ± 0.059	2.234ThrPhe: 2.234 ± 0.042	4.736ThrGly: 4.736 ± 0.057	1.086ThrHis: 1.086 ± 0.033	3.446ThrIle: 3.446 ± 0.067	2.084ThrLys: 2.084 ± 0.051	5.486ThrLeu: 5.486 ± 0.073	1.283ThrMet: 1.283 ± 0.03	1.572ThrAsn: 1.572 ± 0.037	2.728ThrPro: 2.728 ± 0.049	1.403ThrGln: 1.403 ± 0.037	2.725ThrArg: 2.725 ± 0.054	2.721ThrSer: 2.721 ± 0.052	2.63ThrThr: 2.63 ± 0.057	4.728ThrVal: 4.728 ± 0.073	0.593ThrTrp: 0.593 ± 0.024	1.797ThrTyr: 1.797 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	6.759ValAla: 6.759 ± 0.092	0.731ValCys: 0.731 ± 0.03	3.988ValAsp: 3.988 ± 0.069	5.311ValGlu: 5.311 ± 0.073	3.094ValPhe: 3.094 ± 0.059	5.48ValGly: 5.48 ± 0.091	1.713ValHis: 1.713 ± 0.047	5.204ValIle: 5.204 ± 0.08	4.248ValLys: 4.248 ± 0.061	8.112ValLeu: 8.112 ± 0.097	2.128ValMet: 2.128 ± 0.047	2.705ValAsn: 2.705 ± 0.047	3.656ValPro: 3.656 ± 0.059	2.78ValGln: 2.78 ± 0.056	4.521ValArg: 4.521 ± 0.068	4.796ValSer: 4.796 ± 0.065	5.02ValThr: 5.02 ± 0.07	6.417ValVal: 6.417 ± 0.091	0.908ValTrp: 0.908 ± 0.034	2.465ValTyr: 2.465 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.889TrpAla: 0.889 ± 0.027	0.087TrpCys: 0.087 ± 0.009	0.579TrpAsp: 0.579 ± 0.025	0.842TrpGlu: 0.842 ± 0.028	0.493TrpPhe: 0.493 ± 0.021	0.811TrpGly: 0.811 ± 0.03	0.237TrpHis: 0.237 ± 0.014	0.717TrpIle: 0.717 ± 0.024	0.768TrpLys: 0.768 ± 0.03	1.495TrpLeu: 1.495 ± 0.044	0.426TrpMet: 0.426 ± 0.02	0.503TrpAsn: 0.503 ± 0.02	0.399TrpPro: 0.399 ± 0.021	0.506TrpGln: 0.506 ± 0.024	0.729TrpArg: 0.729 ± 0.025	0.641TrpSer: 0.641 ± 0.026	0.597TrpThr: 0.597 ± 0.026	0.789TrpVal: 0.789 ± 0.033	0.184TrpTrp: 0.184 ± 0.013	0.38TrpTyr: 0.38 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.728TyrAla: 2.728 ± 0.047	0.285TyrCys: 0.285 ± 0.017	1.768TyrAsp: 1.768 ± 0.046	2.045TyrGlu: 2.045 ± 0.051	1.258TyrPhe: 1.258 ± 0.039	2.553TyrGly: 2.553 ± 0.051	0.789TyrHis: 0.789 ± 0.033	1.553TyrIle: 1.553 ± 0.035	1.256TyrLys: 1.256 ± 0.038	3.372TyrLeu: 3.372 ± 0.055	0.654TyrMet: 0.654 ± 0.025	0.976TyrAsn: 0.976 ± 0.036	1.496TyrPro: 1.496 ± 0.04	1.285TyrGln: 1.285 ± 0.042	2.295TyrArg: 2.295 ± 0.043	1.471TyrSer: 1.471 ± 0.036	1.615TyrThr: 1.615 ± 0.035	2.537TyrVal: 2.537 ± 0.048	0.396TyrTrp: 0.396 ± 0.02	1.149TyrTyr: 1.149 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3884 proteins (1149012 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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