Torque teno midi virus 7
Average proteome isoelectric point is 7.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7VLW4|A7VLW4_9VIRU Capsid protein OS=Torque teno midi virus 7 OX=2065048 PE=3 SV=1
MM1 pKa = 7.23 QNISADD7 pKa = 3.25 NFFRR11 pKa = 11.84 PTHH14 pKa = 6.07 FNQVTKK20 pKa = 9.71 QQIWMQQIQDD30 pKa = 3.49 SHH32 pKa = 8.93 DD33 pKa = 4.26 NICNCNSCFAHH44 pKa = 6.84 LLANIFPPGHH54 pKa = 7.21 KK55 pKa = 10.53 DD56 pKa = 3.0 RR57 pKa = 11.84 DD58 pKa = 3.49 LSINQILARR67 pKa = 11.84 DD68 pKa = 4.22 LNQPCHH74 pKa = 6.46 SGGTEE79 pKa = 3.77 EE80 pKa = 4.75 EE81 pKa = 4.39 RR82 pKa = 11.84 TGGDD86 pKa = 3.06 TNTRR90 pKa = 11.84 PEE92 pKa = 4.02 NTGAADD98 pKa = 4.1 GPAEE102 pKa = 4.09 EE103 pKa = 4.55 GKK105 pKa = 10.14 HH106 pKa = 5.38 YY107 pKa = 10.85 IEE109 pKa = 5.94 DD110 pKa = 3.89 ADD112 pKa = 4.09 LQEE115 pKa = 5.28 LIAAGEE121 pKa = 4.21 DD122 pKa = 3.18 ATGRR126 pKa = 3.71
Molecular weight: 13.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.613
IPC2_protein 4.66
IPC_protein 4.571
Toseland 4.444
ProMoST 4.724
Dawson 4.558
Bjellqvist 4.698
Wikipedia 4.457
Rodwell 4.444
Grimsley 4.368
Solomon 4.558
Lehninger 4.507
Nozaki 4.673
DTASelect 4.863
Thurlkill 4.469
EMBOSS 4.482
Sillero 4.724
Patrickios 3.694
IPC_peptide 4.558
IPC2_peptide 4.711
IPC2.peptide.svr19 4.64
Protein with the highest isoelectric point:
>tr|A7VLW4|A7VLW4_9VIRU Capsid protein OS=Torque teno midi virus 7 OX=2065048 PE=3 SV=1
MM1 pKa = 7.42 PFWWNRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 8.8 NWWGYY15 pKa = 9.13 KK16 pKa = 9.6 YY17 pKa = 9.34 KK18 pKa = 9.43 TRR20 pKa = 11.84 KK21 pKa = 7.44 YY22 pKa = 10.27 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 WPRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 QTLYY36 pKa = 10.02 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 PTRR43 pKa = 11.84 TNRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 HH52 pKa = 5.39 RR53 pKa = 11.84 KK54 pKa = 8.26 VKK56 pKa = 10.24 RR57 pKa = 11.84 KK58 pKa = 9.99 RR59 pKa = 11.84 KK60 pKa = 7.35 TLTVKK65 pKa = 9.7 QWQPEE70 pKa = 4.42 SIRR73 pKa = 11.84 KK74 pKa = 9.01 CKK76 pKa = 10.32 IIGLEE81 pKa = 3.97 PQLLGAEE88 pKa = 5.26 GSQIDD93 pKa = 4.55 CFTVVKK99 pKa = 9.82 TDD101 pKa = 3.43 YY102 pKa = 11.19 VPAKK106 pKa = 9.64 LPWGGGFSLQNYY118 pKa = 5.72 TLKK121 pKa = 10.81 YY122 pKa = 10.03 LYY124 pKa = 10.32 SEE126 pKa = 4.27 YY127 pKa = 10.18 TYY129 pKa = 11.13 HH130 pKa = 6.63 NNIWTSSNNQKK141 pKa = 10.36 DD142 pKa = 3.57 LCRR145 pKa = 11.84 YY146 pKa = 6.72 TGGYY150 pKa = 9.07 IKK152 pKa = 10.7 LFRR155 pKa = 11.84 HH156 pKa = 5.43 EE157 pKa = 4.26 TQDD160 pKa = 4.32 FIISYY165 pKa = 10.02 DD166 pKa = 3.58 LQPPHH171 pKa = 6.93 EE172 pKa = 4.29 LSKK175 pKa = 10.58 YY176 pKa = 6.97 TFPSCHH182 pKa = 5.8 PHH184 pKa = 6.82 QMLLQKK190 pKa = 9.92 RR191 pKa = 11.84 HH192 pKa = 6.49 KK193 pKa = 10.17 ILLSKK198 pKa = 10.57 QSNPNGKK205 pKa = 9.49 LYY207 pKa = 10.9 KK208 pKa = 9.94 KK209 pKa = 10.32 IKK211 pKa = 9.99 FKK213 pKa = 10.69 PPKK216 pKa = 10.3 QMINKK221 pKa = 8.7 WLFTKK226 pKa = 10.69 NFTPASLIVLKK237 pKa = 9.58 TAVCNFSHH245 pKa = 7.22 ANLSGKK251 pKa = 7.89 NQNMLVSLISLNLSFYY267 pKa = 11.0 KK268 pKa = 10.71 NPAWAQALTASGYY281 pKa = 10.41 KK282 pKa = 10.07 PYY284 pKa = 11.28 DD285 pKa = 4.28 NIPSDD290 pKa = 3.83 LPFVVKK296 pKa = 10.05 QANKK300 pKa = 9.79 EE301 pKa = 4.01 VQKK304 pKa = 11.05 YY305 pKa = 6.92 MKK307 pKa = 9.88 PQATTDD313 pKa = 3.5 YY314 pKa = 8.87 STSISYY320 pKa = 11.0 DD321 pKa = 3.59 DD322 pKa = 3.99 GWFGPLFLSAIKK334 pKa = 10.03 IGKK337 pKa = 8.85 IEE339 pKa = 4.13 QATATHH345 pKa = 6.29 QIIQARR351 pKa = 11.84 YY352 pKa = 8.51 NPNIDD357 pKa = 3.65 SGVGNQIYY365 pKa = 9.93 CASTHH370 pKa = 6.24 AYY372 pKa = 9.33 SWAPPSKK379 pKa = 10.43 DD380 pKa = 3.04 KK381 pKa = 11.16 QLLIEE386 pKa = 5.86 GMPLWLGLFGYY397 pKa = 10.33 YY398 pKa = 10.4 SFVRR402 pKa = 11.84 SVKK405 pKa = 10.7 SEE407 pKa = 4.32 DD408 pKa = 3.18 FMKK411 pKa = 10.94 AHH413 pKa = 6.97 VICIKK418 pKa = 10.82 SPALYY423 pKa = 9.46 CYY425 pKa = 10.13 PEE427 pKa = 5.59 IGSCDD432 pKa = 3.27 TYY434 pKa = 11.79 VFIDD438 pKa = 3.69 PEE440 pKa = 4.7 FISGKK445 pKa = 9.91 RR446 pKa = 11.84 PYY448 pKa = 9.33 EE449 pKa = 3.77 QLITSQQKK457 pKa = 9.76 RR458 pKa = 11.84 LWYY461 pKa = 9.79 PDD463 pKa = 3.32 MSWQKK468 pKa = 9.78 KK469 pKa = 5.66 TMNTLVEE476 pKa = 4.44 AGPYY480 pKa = 8.93 VPKK483 pKa = 10.9 LSDD486 pKa = 3.15 EE487 pKa = 4.42 TFSNWEE493 pKa = 4.0 LNSRR497 pKa = 11.84 YY498 pKa = 9.67 CFYY501 pKa = 10.79 FKK503 pKa = 10.24 WGGPYY508 pKa = 9.46 TDD510 pKa = 4.47 EE511 pKa = 4.76 PEE513 pKa = 4.05 IANPEE518 pKa = 4.09 MLPTYY523 pKa = 9.69 DD524 pKa = 4.99 VPDD527 pKa = 4.06 TVQKK531 pKa = 9.62 TIQIVNPEE539 pKa = 4.12 KK540 pKa = 10.44 QSPEE544 pKa = 4.29 TIIHH548 pKa = 5.73 PWDD551 pKa = 3.47 YY552 pKa = 11.04 RR553 pKa = 11.84 RR554 pKa = 11.84 GLIKK558 pKa = 10.45 EE559 pKa = 4.03 KK560 pKa = 10.44 ALKK563 pKa = 10.34 RR564 pKa = 11.84 MCSNISTDD572 pKa = 3.55 TEE574 pKa = 4.15 FQPVAEE580 pKa = 4.43 TTPKK584 pKa = 10.28 KK585 pKa = 10.33 KK586 pKa = 9.8 PRR588 pKa = 11.84 LRR590 pKa = 11.84 ATLPNPEE597 pKa = 4.78 EE598 pKa = 4.18 EE599 pKa = 4.28 TQEE602 pKa = 4.08 VQKK605 pKa = 11.01 CLLSLCEE612 pKa = 3.64 EE613 pKa = 4.03 STYY616 pKa = 10.5 QEE618 pKa = 4.17 EE619 pKa = 4.84 EE620 pKa = 4.08 EE621 pKa = 4.72 TNLQQLIKK629 pKa = 8.57 QQKK632 pKa = 8.2 YY633 pKa = 6.77 QQQKK637 pKa = 9.66 LKK639 pKa = 10.73 YY640 pKa = 9.52 NMVKK644 pKa = 10.62 LLIDD648 pKa = 4.09 LKK650 pKa = 10.13 EE651 pKa = 4.03 KK652 pKa = 10.59 QRR654 pKa = 11.84 ILQQQTGLLDD664 pKa = 3.39
Molecular weight: 78.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.883
IPC2_protein 9.18
IPC_protein 9.077
Toseland 9.721
ProMoST 9.487
Dawson 9.984
Bjellqvist 9.692
Wikipedia 10.175
Rodwell 10.35
Grimsley 10.072
Solomon 9.999
Lehninger 9.955
Nozaki 9.794
DTASelect 9.677
Thurlkill 9.823
EMBOSS 10.145
Sillero 9.911
Patrickios 5.156
IPC_peptide 9.999
IPC2_peptide 8.361
IPC2.peptide.svr19 8.19
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1321
126
664
330.3
38.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.602 ± 1.064
2.12 ± 0.232
4.542 ± 0.699
5.375 ± 0.396
3.709 ± 0.297
4.996 ± 0.591
2.801 ± 0.569
5.45 ± 0.402
8.478 ± 1.12
7.494 ± 0.7
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.665 ± 0.118
6.056 ± 0.714
6.813 ± 0.499
7.419 ± 0.765
5.753 ± 0.628
6.889 ± 1.162
6.813 ± 0.23
2.498 ± 0.644
1.817 ± 0.531
3.709 ± 1.331
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here