Loktanella fryxellensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Loktanella

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3431 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8J473|A0A1H8J473_9RHOB D-3-phosphoglycerate dehydrogenase OS=Loktanella fryxellensis OX=245187 GN=SAMN04488003_13217 PE=3 SV=1
MM1 pKa = 7.11NVKK4 pKa = 10.33ALLLCLLPLSASAQGFAGLGGDD26 pKa = 3.91ADD28 pKa = 4.4GFSIPQPDD36 pKa = 3.81PQFSFPADD44 pKa = 3.36HH45 pKa = 6.8GAHH48 pKa = 6.58PDD50 pKa = 3.49YY51 pKa = 10.7RR52 pKa = 11.84IEE54 pKa = 3.67WWYY57 pKa = 8.76VTANLTGPDD66 pKa = 3.64GTPYY70 pKa = 10.84GLQWTLFRR78 pKa = 11.84SALAPQTQEE87 pKa = 3.95GWDD90 pKa = 4.32DD91 pKa = 3.46PQLWMGHH98 pKa = 5.77AAVTTPDD105 pKa = 2.74AHH107 pKa = 6.27YY108 pKa = 10.36VAEE111 pKa = 4.12RR112 pKa = 11.84LARR115 pKa = 11.84GGIGQAGVTATPFSAWIDD133 pKa = 3.18DD134 pKa = 3.75WLFDD138 pKa = 5.16GIDD141 pKa = 3.65SGTLRR146 pKa = 11.84ATGADD151 pKa = 3.52FAYY154 pKa = 10.69DD155 pKa = 3.66MQLTAQGPLVAQGIDD170 pKa = 3.49GYY172 pKa = 8.65SVKK175 pKa = 10.46SASGQASYY183 pKa = 11.19YY184 pKa = 9.62YY185 pKa = 9.9SQPFYY190 pKa = 10.85EE191 pKa = 4.56VAGTLTLPDD200 pKa = 3.68GPVAVTGTAWLDD212 pKa = 3.93RR213 pKa = 11.84EE214 pKa = 4.47WSSQPLADD222 pKa = 5.05NQTGWDD228 pKa = 3.47WFSLNFDD235 pKa = 3.87DD236 pKa = 5.62GARR239 pKa = 11.84MMGFRR244 pKa = 11.84LRR246 pKa = 11.84QTDD249 pKa = 3.79GEE251 pKa = 4.71NYY253 pKa = 9.89TSATWIAPDD262 pKa = 3.91GTPTPYY268 pKa = 11.12ADD270 pKa = 3.96GALTATPLEE279 pKa = 4.34EE280 pKa = 4.31SQVGPRR286 pKa = 11.84TVPTTWRR293 pKa = 11.84VEE295 pKa = 4.05LPAQGVDD302 pKa = 3.33VTVAAVNTQAWMDD315 pKa = 3.43TSVPYY320 pKa = 10.09WEE322 pKa = 5.25GPVTIDD328 pKa = 3.28GTRR331 pKa = 11.84SGVGYY336 pKa = 11.02LEE338 pKa = 4.05MTGYY342 pKa = 9.98EE343 pKa = 4.04

Molecular weight: 37.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8AAS4|A0A1H8AAS4_9RHOB L-lactate dehydrogenase (Cytochrome) OS=Loktanella fryxellensis OX=245187 GN=SAMN04488003_10395 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.99NRR15 pKa = 11.84HH16 pKa = 3.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPSGRR28 pKa = 11.84KK29 pKa = 8.93IINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.31GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3431

0

3431

1062454

39

3119

309.7

33.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.716 ± 0.073

0.849 ± 0.013

6.787 ± 0.046

4.288 ± 0.039

3.417 ± 0.028

8.901 ± 0.058

2.107 ± 0.023

4.955 ± 0.03

2.292 ± 0.034

10.038 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.794 ± 0.023

2.288 ± 0.029

5.301 ± 0.035

3.307 ± 0.021

6.958 ± 0.043

4.508 ± 0.028

6.32 ± 0.047

7.739 ± 0.037

1.387 ± 0.021

2.048 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski