Aspergillus candidus
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9639 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I2F865|A0A2I2F865_9EURO Zn(2)-C6 fungal-type domain-containing protein OS=Aspergillus candidus OX=41067 GN=BDW47DRAFT_132620 PE=4 SV=1
MM1 pKa = 6.92 VRR3 pKa = 11.84 QAAFVIPALFLQAAVARR20 pKa = 11.84 PAGAEE25 pKa = 4.03 LPPPGYY31 pKa = 9.24 EE32 pKa = 3.93 VNVVEE37 pKa = 4.01 VCYY40 pKa = 9.92 PVVIPGDD47 pKa = 3.87 DD48 pKa = 3.55 NRR50 pKa = 11.84 GLGSGGAHH58 pKa = 6.56 PPGSLEE64 pKa = 3.9 IGQGPSNGTSPGVHH78 pKa = 6.25 GGGNGGNGGNGSNGGTGLGALGGGNGGNGGKK109 pKa = 9.9 NGGSTGSHH117 pKa = 6.31 GGDD120 pKa = 2.67 SGANNGANTSGPRR133 pKa = 11.84 GDD135 pKa = 3.96 NGSTGGSTGGPGGDD149 pKa = 3.06 NGSNVGGSTGGPGGSTGGSNGGSNGGSAGGSGGDD183 pKa = 3.22 NGGNGGGSTGGPGGDD198 pKa = 3.1 NGGNGGGGTGGPGGDD213 pKa = 3.19 NGGNGGGSNTGGAGEE228 pKa = 4.61 SSTGTSISQPTTNIIPLPSTSEE250 pKa = 3.53 WCPPVTVTDD259 pKa = 4.41 CPIITPSDD267 pKa = 3.94 CPVVTDD273 pKa = 3.51 VTTITITTAPPGSSGVPGLPTTDD296 pKa = 4.11 CLTDD300 pKa = 3.24 VSTITITIPGSSGLPVSPTDD320 pKa = 3.69 CPTVTEE326 pKa = 4.56 PGQTVTLPGQTITEE340 pKa = 4.52 PGQTLTLPGQTITLPGQTITEE361 pKa = 4.47 PGLTVTDD368 pKa = 4.45 PGQTLTLPPSQVKK381 pKa = 9.33 PP382 pKa = 3.62
Molecular weight: 35.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.859
IPC_protein 3.834
Toseland 3.617
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.528
Solomon 3.821
Lehninger 3.77
Nozaki 3.948
DTASelect 4.177
Thurlkill 3.681
EMBOSS 3.783
Sillero 3.961
Patrickios 0.998
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.846
Protein with the highest isoelectric point:
>tr|A0A2I2FAT2|A0A2I2FAT2_9EURO Protein YIP OS=Aspergillus candidus OX=41067 GN=BDW47DRAFT_106210 PE=3 SV=1
MM1 pKa = 7.48 LAASLATSPVTAPLPTVVPSAPPARR26 pKa = 11.84 SATSAPRR33 pKa = 11.84 LATSLGTAPTTRR45 pKa = 11.84 PLPSNPPRR53 pKa = 11.84 LVPPPPFLLLPLPSRR68 pKa = 11.84 RR69 pKa = 11.84 LPKK72 pKa = 10.47 SPLLPRR78 pKa = 11.84 LPLPLSHH85 pKa = 7.17 KK86 pKa = 10.57 KK87 pKa = 8.53 STLL90 pKa = 3.35
Molecular weight: 9.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.471
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.457
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.193
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.103
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9639
0
9639
4627908
49
5484
480.1
53.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.622 ± 0.024
1.259 ± 0.01
5.835 ± 0.017
6.003 ± 0.023
3.702 ± 0.014
6.996 ± 0.025
2.519 ± 0.012
4.618 ± 0.019
4.401 ± 0.019
8.985 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.184 ± 0.009
3.437 ± 0.013
6.434 ± 0.031
4.006 ± 0.018
6.405 ± 0.024
8.25 ± 0.026
5.889 ± 0.015
6.284 ± 0.02
1.44 ± 0.008
2.727 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here