Microbacterium phage Floof

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4Q5K2|A0A2Z4Q5K2_9CAUD Terminase small subunit OS=Microbacterium phage Floof OX=2201433 GN=1 PE=4 SV=1
MM1 pKa = 8.12IPEE4 pKa = 4.71DD5 pKa = 4.1LFNAYY10 pKa = 9.82EE11 pKa = 4.36PDD13 pKa = 3.78DD14 pKa = 4.31PKK16 pKa = 11.17LYY18 pKa = 10.87SLLEE22 pKa = 4.03AAA24 pKa = 5.53

Molecular weight: 2.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q4Q2|A0A2Z4Q4Q2_9CAUD NrdH-like glutaredoxin OS=Microbacterium phage Floof OX=2201433 GN=43 PE=4 SV=1
MM1 pKa = 7.82IDD3 pKa = 3.59HH4 pKa = 7.11LTPEE8 pKa = 4.65AGLPLAALLWAVVALPHH25 pKa = 5.08VARR28 pKa = 11.84WNRR31 pKa = 11.84ARR33 pKa = 11.84RR34 pKa = 11.84AARR37 pKa = 11.84KK38 pKa = 6.88EE39 pKa = 4.23TCASLPTSVV48 pKa = 3.99

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

14971

24

973

187.1

20.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.606 ± 0.398

0.501 ± 0.086

6.239 ± 0.338

5.905 ± 0.31

3.033 ± 0.195

7.989 ± 0.359

2.064 ± 0.168

4.242 ± 0.271

3.393 ± 0.195

8.957 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.817 ± 0.092

2.371 ± 0.254

5.537 ± 0.299

2.966 ± 0.166

7.521 ± 0.482

5.798 ± 0.278

7.267 ± 0.401

6.813 ± 0.254

1.657 ± 0.151

2.324 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski