Proteome-pI 2.0 - Proteome Isoelectric Point Database

Streptococcus pneumoniae (strain ATCC BAA-255 / R6)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus pneumoniae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 2030 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|Q8CY79|Q8CY79_STRR6 Uncharacterized protein OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr1883 PE=4 SV=1

MM1 pKa = 7.43ILWSFDD7 pKa = 3.3FANDD11 pKa = 3.28HH12 pKa = 5.8AHH14 pKa = 6.95AFFMDD19 pKa = 3.49NVEE22 pKa = 3.96WSHH25 pKa = 6.61ADD27 pKa = 3.12SYY29 pKa = 11.51FLSFVSDD36 pKa = 3.96DD37 pKa = 3.43VEE39 pKa = 3.85EE40 pKa = 5.29RR41 pKa = 11.84YY42 pKa = 8.45TEE44 pKa = 4.06NVYY47 pKa = 10.99LDD49 pKa = 3.88SLSVKK54 pKa = 10.32QKK56 pKa = 10.61FKK58 pKa = 11.08FIFDD62 pKa = 4.68FGDD65 pKa = 2.97EE66 pKa = 4.14WRR68 pKa = 11.84FEE70 pKa = 4.31CQVLRR75 pKa = 11.84EE76 pKa = 4.27IEE78 pKa = 4.34TEE80 pKa = 3.93DD81 pKa = 3.34EE82 pKa = 3.63EE83 pKa = 6.14AYY85 pKa = 10.53LVRR88 pKa = 11.84SVGTSPEE95 pKa = 3.93QYY97 pKa = 9.93PDD99 pKa = 3.41YY100 pKa = 11.41DD101 pKa = 3.55GFDD104 pKa = 3.56YY105 pKa = 11.34EE106 pKa = 4.37EE107 pKa = 4.1WW108 pKa = 4.25

Molecular weight: 13.11 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>sp|P66908|TGT_STRR6 Queuine tRNA-ribosyltransferase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=tgt PE=3 SV=1

MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.24QPSKK9 pKa = 9.82LRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 8.57HH16 pKa = 4.75GFRR19 pKa = 11.84NRR21 pKa = 11.84MSTKK25 pKa = 9.22NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.87GRR39 pKa = 11.84KK40 pKa = 8.75VLAAA44 pKa = 4.31

Molecular weight: 5.27 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
2030	0	2030	587055	20	2551	289.2	32.5

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
7.328 ± 0.073	0.604 ± 0.014	5.502 ± 0.045	7.253 ± 0.063	4.656 ± 0.046	6.589 ± 0.062	1.928 ± 0.022	7.286 ± 0.063	6.866 ± 0.059	10.241 ± 0.076

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.501 ± 0.032	4.414 ± 0.045	3.311 ± 0.032	4.07 ± 0.045	4.096 ± 0.042	6.212 ± 0.054	5.434 ± 0.04	6.959 ± 0.05	0.928 ± 0.021	3.822 ± 0.036

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski