Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1)
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3594 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0LB97|A0LB97_MAGMM Uncharacterized protein OS=Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1) OX=156889 GN=Mmc1_2747 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 10.25 KK3 pKa = 9.12 WVCNACGFEE12 pKa = 3.93 YY13 pKa = 10.84 DD14 pKa = 3.86 EE15 pKa = 5.35 AVGLPEE21 pKa = 5.7 DD22 pKa = 5.38 GIPAGTAWADD32 pKa = 3.59 VPADD36 pKa = 4.09 WVCPDD41 pKa = 3.93 CGVGKK46 pKa = 10.34 DD47 pKa = 3.59 EE48 pKa = 5.08 FSMVEE53 pKa = 3.7 AA54 pKa = 5.08
Molecular weight: 5.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.808
IPC_protein 3.681
Toseland 3.49
ProMoST 3.872
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.414
Solomon 3.643
Lehninger 3.605
Nozaki 3.834
DTASelect 3.999
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.808
Patrickios 0.604
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>sp|A0L3L9|BIOC_MAGMM Malonyl-[acyl-carrier protein] O-methyltransferase OS=Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1) OX=156889 GN=bioC PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.41 IRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.09 RR14 pKa = 11.84 THH16 pKa = 6.0 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.13 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.15 GRR39 pKa = 11.84 KK40 pKa = 8.93 ALIPP44 pKa = 4.1
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.735
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.457
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3594
0
3594
1325866
37
15245
368.9
40.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.944 ± 0.063
0.947 ± 0.023
5.274 ± 0.089
6.135 ± 0.072
3.46 ± 0.032
7.817 ± 0.095
2.635 ± 0.043
4.991 ± 0.036
3.903 ± 0.06
10.975 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.832 ± 0.037
3.167 ± 0.038
4.791 ± 0.064
5.15 ± 0.057
5.989 ± 0.095
5.78 ± 0.099
5.294 ± 0.095
7.058 ± 0.047
1.38 ± 0.021
2.48 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here