Niabella soli DSM 19437
Average proteome isoelectric point is 7.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4110 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W0F6P7|W0F6P7_9BACT Uncharacterized protein OS=Niabella soli DSM 19437 OX=929713 GN=NIASO_08850 PE=4 SV=1
MM1 pKa = 8.03 DD2 pKa = 4.29 IEE4 pKa = 5.21 LYY6 pKa = 8.82 VHH8 pKa = 6.93 AVFVCSDD15 pKa = 3.25 AGNNDD20 pKa = 3.44 ILPSAAAGSGLPEE33 pKa = 5.27 DD34 pKa = 5.2 DD35 pKa = 4.92 GDD37 pKa = 4.71 IEE39 pKa = 4.29 EE40 pKa = 4.04 QSIRR44 pKa = 11.84 CSPTSPQPSPLNQPP58 pKa = 3.43
Molecular weight: 6.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.845
IPC2_protein 3.617
IPC_protein 3.49
Toseland 3.312
ProMoST 3.719
Dawson 3.516
Bjellqvist 3.694
Wikipedia 3.503
Rodwell 3.35
Grimsley 3.236
Solomon 3.452
Lehninger 3.414
Nozaki 3.681
DTASelect 3.834
Thurlkill 3.414
EMBOSS 3.503
Sillero 3.63
Patrickios 0.477
IPC_peptide 3.452
IPC2_peptide 3.592
IPC2.peptide.svr19 3.672
Protein with the highest isoelectric point:
>tr|W0F8Y2|W0F8Y2_9BACT Uncharacterized protein OS=Niabella soli DSM 19437 OX=929713 GN=NIASO_14900 PE=4 SV=1
MM1 pKa = 7.56 VFDD4 pKa = 5.34 EE5 pKa = 5.95 GPQQVQCWGFFLPIMLIMTNNAQLRR30 pKa = 11.84 LHH32 pKa = 7.05 KK33 pKa = 9.99 KK34 pKa = 10.03 RR35 pKa = 11.84 KK36 pKa = 8.13 IFEE39 pKa = 4.15 NRR41 pKa = 11.84 RR42 pKa = 11.84 SACNYY47 pKa = 8.95 ARR49 pKa = 11.84 NGFIWTGFDD58 pKa = 3.56 AVILRR63 pKa = 11.84 LPLQWQHH70 pKa = 5.87 RR71 pKa = 11.84 QGQITTVVAA80 pKa = 4.14
Molecular weight: 9.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.502
IPC_protein 10.145
Toseland 10.628
ProMoST 10.277
Dawson 10.716
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.935
Grimsley 10.745
Solomon 10.847
Lehninger 10.818
Nozaki 10.643
DTASelect 10.394
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.657
Patrickios 10.774
IPC_peptide 10.847
IPC2_peptide 9.663
IPC2.peptide.svr19 8.515
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4110
0
4110
1373064
29
2870
334.1
37.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.237 ± 0.039
0.846 ± 0.012
5.17 ± 0.022
5.311 ± 0.041
4.835 ± 0.027
7.184 ± 0.034
1.885 ± 0.018
6.791 ± 0.03
6.935 ± 0.033
9.423 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.311 ± 0.014
5.347 ± 0.038
4.164 ± 0.021
3.876 ± 0.02
4.242 ± 0.025
5.868 ± 0.028
5.799 ± 0.025
6.319 ± 0.024
1.371 ± 0.015
4.086 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here