Lichtheimia corymbifera JMRC:FSU:9682
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13257 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A068SC33|A0A068SC33_9FUNG Uncharacterized protein OS=Lichtheimia corymbifera JMRC:FSU:9682 OX=1263082 GN=LCOR_10216.1 PE=4 SV=1
MM1 pKa = 7.86 RR2 pKa = 11.84 LLILLLILALAAMVFAQDD20 pKa = 3.92 PGAGDD25 pKa = 3.54 SGTGDD30 pKa = 3.29 GVGGDD35 pKa = 3.71 TGGDD39 pKa = 3.32 TGSDD43 pKa = 3.28 GSTGGDD49 pKa = 3.24 GSTGAGDD56 pKa = 3.74 GTTGGDD62 pKa = 3.24 SGTGGGDD69 pKa = 3.09 TSTGGDD75 pKa = 3.57 GSTGGDD81 pKa = 3.44 GSTGGDD87 pKa = 3.44 GSTGGDD93 pKa = 3.41 GSTGGGDD100 pKa = 3.32 AGTGDD105 pKa = 4.11 GSTGGDD111 pKa = 3.44 TQQPSDD117 pKa = 4.85 DD118 pKa = 4.46 GSTTPNPTGDD128 pKa = 3.77 GTTSPSPSDD137 pKa = 4.99 DD138 pKa = 3.33 GTTDD142 pKa = 3.31 PQQPTDD148 pKa = 3.53 QPSDD152 pKa = 3.17 GGEE155 pKa = 3.98 QQQPSPTDD163 pKa = 3.47 PEE165 pKa = 4.32 TAPDD169 pKa = 3.76 ATPSGDD175 pKa = 3.75 TVDD178 pKa = 4.54 EE179 pKa = 4.55 AACQALKK186 pKa = 10.56 QIYY189 pKa = 10.16 NSLNGNSWTVSNGWSGDD206 pKa = 3.76 DD207 pKa = 3.75 MTSCCNGWFGVSCIMGSVSKK227 pKa = 10.71 IQLPYY232 pKa = 10.91 NNLAGEE238 pKa = 4.32 LPDD241 pKa = 4.17 VFGSLQSLTNLDD253 pKa = 3.89 LSHH256 pKa = 6.22 NQISGSLPGSLTNMPNLRR274 pKa = 11.84 FINVDD279 pKa = 3.41 VNNLSGGLIAFGQTPMLQSIHH300 pKa = 6.03 MKK302 pKa = 10.15 NNSFTGSIPDD312 pKa = 2.89 SWGNIGTLNDD322 pKa = 3.57 IYY324 pKa = 11.12 LSNNQLSGPFPMSILNLGQLTTLAIDD350 pKa = 3.35 NNQFNGIVPPQIGNLGSLKK369 pKa = 10.48 VLNIRR374 pKa = 11.84 QNHH377 pKa = 5.85 LLGNIPASIGNLTSLTNLLMSDD399 pKa = 3.18 MMLNGTIPEE408 pKa = 4.17 SLYY411 pKa = 11.21 GLSNLQYY418 pKa = 11.25 LDD420 pKa = 4.11 LSHH423 pKa = 6.78 NRR425 pKa = 11.84 LTGEE429 pKa = 3.91 ISDD432 pKa = 4.95 KK433 pKa = 10.96 LGQLVNVKK441 pKa = 10.52 KK442 pKa = 11.0 LMLGRR447 pKa = 11.84 NGLTGPIPDD456 pKa = 4.18 SLGALAQLEE465 pKa = 4.37 YY466 pKa = 11.26 VQLNYY471 pKa = 10.8 NQLNGQFPSFMAPRR485 pKa = 11.84 ALGYY489 pKa = 10.77 CYY491 pKa = 7.47 MTPNQFQLCPDD502 pKa = 3.64 DD503 pKa = 4.88 TIVHH507 pKa = 6.15 NPEE510 pKa = 3.77 SMAFQCATDD519 pKa = 4.03 CFIGTTRR526 pKa = 11.84 PGAGSSTRR534 pKa = 11.84 ANLFVTIALVLLALFFYY551 pKa = 10.94 CC552 pKa = 5.99
Molecular weight: 56.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.681
IPC_protein 3.745
Toseland 3.49
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.732
Rodwell 3.554
Grimsley 3.401
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.202
Thurlkill 3.554
EMBOSS 3.745
Sillero 3.872
Patrickios 0.846
IPC_peptide 3.745
IPC2_peptide 3.834
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A068RSL7|A0A068RSL7_9FUNG Uncharacterized protein OS=Lichtheimia corymbifera JMRC:FSU:9682 OX=1263082 GN=LCOR_04152.1 PE=4 SV=1
MM1 pKa = 7.26 HH2 pKa = 7.14 MKK4 pKa = 10.46 GKK6 pKa = 8.7 RR7 pKa = 11.84 TRR9 pKa = 11.84 TRR11 pKa = 11.84 TRR13 pKa = 11.84 TRR15 pKa = 11.84 TRR17 pKa = 11.84 THH19 pKa = 5.65 ICAHH23 pKa = 5.6 IRR25 pKa = 11.84 AHH27 pKa = 5.83 IRR29 pKa = 11.84 THH31 pKa = 4.77 IRR33 pKa = 11.84 TRR35 pKa = 11.84 TRR37 pKa = 11.84 TRR39 pKa = 11.84 TRR41 pKa = 11.84 TRR43 pKa = 11.84 THH45 pKa = 5.16 STHH48 pKa = 5.75 IRR50 pKa = 11.84 THH52 pKa = 5.33 IRR54 pKa = 11.84 THH56 pKa = 5.44 IRR58 pKa = 11.84 THH60 pKa = 4.82 IRR62 pKa = 11.84 TRR64 pKa = 11.84 TRR66 pKa = 11.84 TRR68 pKa = 11.84 TRR70 pKa = 11.84 TRR72 pKa = 11.84 THH74 pKa = 5.16 STHH77 pKa = 5.75 IRR79 pKa = 11.84 THH81 pKa = 5.33 IRR83 pKa = 11.84 THH85 pKa = 5.44 IRR87 pKa = 11.84 THH89 pKa = 4.82 IRR91 pKa = 11.84 TRR93 pKa = 11.84 TRR95 pKa = 11.84 TRR97 pKa = 11.84 TRR99 pKa = 11.84 TRR101 pKa = 11.84 TRR103 pKa = 11.84 TRR105 pKa = 11.84 SEE107 pKa = 3.71
Molecular weight: 13.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.852
Toseland 13.013
ProMoST 13.51
Dawson 13.013
Bjellqvist 13.013
Wikipedia 13.495
Rodwell 12.544
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 13.013
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.252
IPC_peptide 13.525
IPC2_peptide 12.501
IPC2.peptide.svr19 9.219
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12138
1119
13257
5717124
66
6893
431.3
48.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.451 ± 0.019
1.446 ± 0.01
6.051 ± 0.016
5.815 ± 0.023
3.633 ± 0.014
5.404 ± 0.021
3.02 ± 0.014
5.333 ± 0.015
5.15 ± 0.021
8.791 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.708 ± 0.009
4.241 ± 0.012
5.455 ± 0.025
4.967 ± 0.022
5.666 ± 0.018
8.255 ± 0.027
6.331 ± 0.018
5.878 ± 0.018
1.248 ± 0.007
3.154 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here