Ustilaginoidea virens partitivirus 2
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S5M8T3|S5M8T3_9VIRU Uncharacterized protein OS=Ustilaginoidea virens partitivirus 2 OX=1415665 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 10.65 NEE4 pKa = 3.98 IAFQPGKK11 pKa = 9.65 FSPVIEE17 pKa = 4.17 VSQRR21 pKa = 11.84 QSGVPAPADD30 pKa = 3.37 TSFDD34 pKa = 3.48 GLEE37 pKa = 4.1 EE38 pKa = 4.19 VALTTLQVATSSARR52 pKa = 11.84 GSGAVIQDD60 pKa = 3.71 SVPLSKK66 pKa = 10.87 LSMVIGYY73 pKa = 9.11 LLAARR78 pKa = 11.84 NIAEE82 pKa = 3.96 QLLFIGVSTRR92 pKa = 11.84 MSTPLRR98 pKa = 11.84 APNGNFLEE106 pKa = 5.13 PIVRR110 pKa = 11.84 AYY112 pKa = 11.01 NFYY115 pKa = 11.14 GHH117 pKa = 6.84 FEE119 pKa = 4.33 HH120 pKa = 7.35 EE121 pKa = 4.6 SKK123 pKa = 9.93 TFVTRR128 pKa = 11.84 GLEE131 pKa = 4.09 TLFIRR136 pKa = 11.84 YY137 pKa = 8.79 LFGLRR142 pKa = 11.84 QFATTGVSDD151 pKa = 4.61 GSAHH155 pKa = 5.0 TVRR158 pKa = 11.84 VLSNQNHH165 pKa = 5.62 NFSDD169 pKa = 3.5 IDD171 pKa = 3.86 LDD173 pKa = 4.46 FYY175 pKa = 11.55 YY176 pKa = 10.64 LSEE179 pKa = 5.01 AGEE182 pKa = 3.99 IAFGKK187 pKa = 10.1 RR188 pKa = 11.84 YY189 pKa = 8.78 PLKK192 pKa = 10.01 EE193 pKa = 3.95 YY194 pKa = 10.48 LIHH197 pKa = 5.8 LVKK200 pKa = 10.64 YY201 pKa = 10.42 ASDD204 pKa = 3.57 FGDD207 pKa = 3.17 VDD209 pKa = 3.4 EE210 pKa = 6.36 RR211 pKa = 11.84 IPLLQRR217 pKa = 11.84 ILDD220 pKa = 3.76 VTTEE224 pKa = 4.11 DD225 pKa = 4.42 GLNQFLRR232 pKa = 11.84 YY233 pKa = 9.57 ARR235 pKa = 11.84 RR236 pKa = 11.84 LPDD239 pKa = 2.97 WQTPPRR245 pKa = 11.84 EE246 pKa = 4.16 FTPDD250 pKa = 3.22 PSDD253 pKa = 3.21 KK254 pKa = 10.59 HH255 pKa = 6.76 KK256 pKa = 11.32 SAMKK260 pKa = 9.87 EE261 pKa = 3.92 LFGAAYY267 pKa = 8.26 STAGQSIPVSKK278 pKa = 9.42 FTEE281 pKa = 4.71 SINLAYY287 pKa = 10.45 ALLRR291 pKa = 11.84 RR292 pKa = 11.84 VSLEE296 pKa = 3.48 RR297 pKa = 11.84 QYY299 pKa = 12.08 GMKK302 pKa = 9.41 TVQIPRR308 pKa = 11.84 YY309 pKa = 8.61 EE310 pKa = 4.43 GGSPSQMASYY320 pKa = 10.69 QDD322 pKa = 4.16 DD323 pKa = 4.1 VLFSTIPLSLADD335 pKa = 3.38 STAAVGFTTSYY346 pKa = 11.25 GSLARR351 pKa = 11.84 YY352 pKa = 7.09 TSAPGFEE359 pKa = 4.16 KK360 pKa = 10.91 DD361 pKa = 3.32 EE362 pKa = 4.27 LVRR365 pKa = 11.84 EE366 pKa = 4.33 LVSQSLVLRR375 pKa = 4.52
Molecular weight: 41.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.707
IPC2_protein 5.703
IPC_protein 5.715
Toseland 6.135
ProMoST 6.02
Dawson 5.919
Bjellqvist 5.944
Wikipedia 5.893
Rodwell 5.881
Grimsley 6.338
Solomon 5.919
Lehninger 5.893
Nozaki 6.148
DTASelect 6.338
Thurlkill 6.3
EMBOSS 6.275
Sillero 6.249
Patrickios 4.202
IPC_peptide 5.931
IPC2_peptide 6.237
IPC2.peptide.svr19 6.212
Protein with the highest isoelectric point:
>tr|S5M8T3|S5M8T3_9VIRU Uncharacterized protein OS=Ustilaginoidea virens partitivirus 2 OX=1415665 PE=4 SV=1
MM1 pKa = 7.69 NSFEE5 pKa = 4.45 NFGSFKK11 pKa = 10.93 LSADD15 pKa = 3.55 EE16 pKa = 4.4 LAATAVPPTPWNNVFRR32 pKa = 11.84 YY33 pKa = 8.37 ITDD36 pKa = 3.46 AKK38 pKa = 10.39 RR39 pKa = 11.84 FPGYY43 pKa = 10.11 KK44 pKa = 9.48 RR45 pKa = 11.84 GILRR49 pKa = 11.84 QTQLYY54 pKa = 9.83 DD55 pKa = 3.7 PYY57 pKa = 11.55 VNAALKK63 pKa = 10.55 SFSPEE68 pKa = 3.48 LHH70 pKa = 7.06 DD71 pKa = 5.46 SIKK74 pKa = 10.73 GYY76 pKa = 8.53 TRR78 pKa = 11.84 APGDD82 pKa = 3.09 EE83 pKa = 3.73 WDD85 pKa = 3.64 VYY87 pKa = 11.14 EE88 pKa = 5.59 RR89 pKa = 11.84 LTRR92 pKa = 11.84 YY93 pKa = 9.63 DD94 pKa = 3.87 KK95 pKa = 11.54 SPLAPVDD102 pKa = 3.38 NPRR105 pKa = 11.84 FKK107 pKa = 10.92 ACYY110 pKa = 9.41 DD111 pKa = 3.45 AALSDD116 pKa = 3.61 VMKK119 pKa = 10.23 EE120 pKa = 4.01 FKK122 pKa = 10.77 LRR124 pKa = 11.84 DD125 pKa = 3.48 PVVPHH130 pKa = 7.26 WILDD134 pKa = 3.42 VDD136 pKa = 4.35 LVKK139 pKa = 10.22 NTSSGFPHH147 pKa = 6.64 FTRR150 pKa = 11.84 KK151 pKa = 9.94 GDD153 pKa = 3.35 ILDD156 pKa = 3.89 QIRR159 pKa = 11.84 QEE161 pKa = 4.32 GRR163 pKa = 11.84 SHH165 pKa = 6.46 FHH167 pKa = 5.97 LLKK170 pKa = 10.56 RR171 pKa = 11.84 LPLWRR176 pKa = 11.84 VPLLPCTPATRR187 pKa = 11.84 GGLADD192 pKa = 3.06 ITEE195 pKa = 4.47 PKK197 pKa = 9.07 TRR199 pKa = 11.84 LVWMYY204 pKa = 8.82 PAAMTAVEE212 pKa = 4.79 AVFAQPLIDD221 pKa = 4.11 GLFSEE226 pKa = 4.64 KK227 pKa = 10.41 SEE229 pKa = 3.94 YY230 pKa = 10.82 LITGVDD236 pKa = 3.35 TKK238 pKa = 11.16 HH239 pKa = 6.82 RR240 pKa = 11.84 IQRR243 pKa = 11.84 YY244 pKa = 8.99 LSLLSEE250 pKa = 4.23 DD251 pKa = 3.44 TGRR254 pKa = 11.84 LGVGLDD260 pKa = 3.73 FKK262 pKa = 11.63 SFDD265 pKa = 3.58 TLRR268 pKa = 11.84 CNWLIRR274 pKa = 11.84 DD275 pKa = 3.82 AFDD278 pKa = 3.26 VLKK281 pKa = 10.97 QNVYY285 pKa = 10.23 FSGYY289 pKa = 10.28 YY290 pKa = 10.28 DD291 pKa = 3.93 DD292 pKa = 5.31 TNGLQTFGPGKK303 pKa = 8.11 TEE305 pKa = 3.93 RR306 pKa = 11.84 LEE308 pKa = 4.3 HH309 pKa = 5.8 AWSNIVEE316 pKa = 4.36 YY317 pKa = 10.07 FIHH320 pKa = 6.56 TPILLPNGRR329 pKa = 11.84 CVNKK333 pKa = 9.15 HH334 pKa = 5.06 TGVPSGSHH342 pKa = 4.96 FTNLIDD348 pKa = 5.71 SIICRR353 pKa = 11.84 ILIKK357 pKa = 10.0 TFSLYY362 pKa = 10.67 CSIPISNLRR371 pKa = 11.84 TNGDD375 pKa = 3.28 DD376 pKa = 3.77 SAFHH380 pKa = 6.83 VYY382 pKa = 10.22 EE383 pKa = 5.59 DD384 pKa = 3.92 YY385 pKa = 11.42 ASDD388 pKa = 3.95 IILRR392 pKa = 11.84 AAGFFKK398 pKa = 10.6 EE399 pKa = 4.32 FFGMTINTDD408 pKa = 3.36 KK409 pKa = 11.28 SCVAGSPSEE418 pKa = 3.93 MHH420 pKa = 6.3 VSGTRR425 pKa = 11.84 WTGLRR430 pKa = 11.84 PTRR433 pKa = 11.84 STQEE437 pKa = 3.45 WMMLAAYY444 pKa = 9.87 GEE446 pKa = 4.64 TYY448 pKa = 10.91 SRR450 pKa = 11.84 IPFDD454 pKa = 3.25 SFQRR458 pKa = 11.84 LLGLGLSGGFGDD470 pKa = 3.94 STFTRR475 pKa = 11.84 FFDD478 pKa = 3.97 YY479 pKa = 10.6 FQTGYY484 pKa = 10.96 DD485 pKa = 3.78 CRR487 pKa = 11.84 HH488 pKa = 6.07 GPNLLNWKK496 pKa = 9.6 KK497 pKa = 10.64 LRR499 pKa = 11.84 FLQQIFSIEE508 pKa = 4.12 EE509 pKa = 3.82 LPLVYY514 pKa = 10.25 KK515 pKa = 10.72 QGAKK519 pKa = 6.04 TTLRR523 pKa = 11.84 LRR525 pKa = 11.84 LLVTT529 pKa = 4.34
Molecular weight: 60.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.36
IPC2_protein 7.732
IPC_protein 7.6
Toseland 7.337
ProMoST 8.112
Dawson 8.302
Bjellqvist 8.58
Wikipedia 8.244
Rodwell 8.317
Grimsley 7.41
Solomon 8.375
Lehninger 8.39
Nozaki 8.697
DTASelect 8.361
Thurlkill 8.404
EMBOSS 8.492
Sillero 8.697
Patrickios 4.291
IPC_peptide 8.375
IPC2_peptide 7.541
IPC2.peptide.svr19 7.726
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
904
375
529
452.0
51.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.527 ± 0.918
0.885 ± 0.551
5.973 ± 0.565
4.867 ± 0.623
6.305 ± 0.273
6.858 ± 0.119
2.102 ± 0.313
4.867 ± 0.208
4.314 ± 0.528
10.73 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.549 ± 0.032
3.208 ± 0.337
5.531 ± 0.123
3.429 ± 0.688
6.637 ± 0.148
8.075 ± 1.116
6.858 ± 0.286
5.642 ± 0.639
1.217 ± 0.592
4.425 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here