Cybaeus spider associated circular virus 1
Average proteome isoelectric point is 7.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPA9|A0A346BPA9_9VIRU Putative capsid protein OS=Cybaeus spider associated circular virus 1 OX=2293277 PE=4 SV=1
MM1 pKa = 7.73 NSRR4 pKa = 11.84 SKK6 pKa = 10.22 NWCFTSYY13 pKa = 10.77 NVEE16 pKa = 4.19 SEE18 pKa = 4.38 PFLQQRR24 pKa = 11.84 NKK26 pKa = 9.87 IQYY29 pKa = 8.53 LIWGRR34 pKa = 11.84 EE35 pKa = 3.86 TCPDD39 pKa = 3.34 TNRR42 pKa = 11.84 PHH44 pKa = 6.61 LQGFVAFKK52 pKa = 10.92 VRR54 pKa = 11.84 TKK56 pKa = 10.73 FSTLKK61 pKa = 10.47 NFEE64 pKa = 4.14 NFHH67 pKa = 5.77 FEE69 pKa = 4.0 KK70 pKa = 10.43 TKK72 pKa = 9.79 GTIHH76 pKa = 6.6 EE77 pKa = 4.16 AVEE80 pKa = 4.17 YY81 pKa = 10.16 CKK83 pKa = 10.56 KK84 pKa = 10.85 DD85 pKa = 3.2 GDD87 pKa = 3.86 FTEE90 pKa = 4.85 YY91 pKa = 11.05 GVLPSATGNNSAFKK105 pKa = 10.43 QVLAFAKK112 pKa = 9.97 KK113 pKa = 10.41 GEE115 pKa = 3.87 ISAIEE120 pKa = 4.3 DD121 pKa = 3.79 EE122 pKa = 4.86 HH123 pKa = 7.79 PGIYY127 pKa = 9.42 IRR129 pKa = 11.84 YY130 pKa = 8.5 KK131 pKa = 9.73 RR132 pKa = 11.84 TLEE135 pKa = 3.86 SLAVYY140 pKa = 7.73 NTSEE144 pKa = 4.57 LEE146 pKa = 4.04 NSCGVWIHH154 pKa = 6.14 GPPRR158 pKa = 11.84 CGKK161 pKa = 10.15 DD162 pKa = 3.23 YY163 pKa = 11.22 AVRR166 pKa = 11.84 QLGNVYY172 pKa = 10.55 NKK174 pKa = 10.2 GMNKK178 pKa = 8.32 WWDD181 pKa = 3.53 NYY183 pKa = 10.04 KK184 pKa = 10.86 GEE186 pKa = 4.83 PIVLLSDD193 pKa = 3.67 VQPDD197 pKa = 3.36 QAKK200 pKa = 9.28 WLGHH204 pKa = 5.63 FLKK207 pKa = 10.28 IWADD211 pKa = 3.78 RR212 pKa = 11.84 YY213 pKa = 11.07 AFNAEE218 pKa = 4.28 IKK220 pKa = 10.56 CGTIYY225 pKa = 10.25 IRR227 pKa = 11.84 PKK229 pKa = 10.03 QFYY232 pKa = 8.06 VTSNFSIDD240 pKa = 3.71 EE241 pKa = 4.04 IFEE244 pKa = 4.23 GKK246 pKa = 9.68 IKK248 pKa = 10.25 EE249 pKa = 4.34 AIAARR254 pKa = 11.84 FDD256 pKa = 3.73 EE257 pKa = 5.44 LDD259 pKa = 3.39 FSTDD263 pKa = 2.87 TNIIKK268 pKa = 10.34 NRR270 pKa = 11.84 LEE272 pKa = 4.34 SIAPRR277 pKa = 11.84 RR278 pKa = 11.84 VLACLLDD285 pKa = 3.73 RR286 pKa = 11.84 QNIVAPAAVEE296 pKa = 4.43 VTSDD300 pKa = 3.44 PQPFQLHH307 pKa = 7.01 DD308 pKa = 3.98 GFSSEE313 pKa = 4.23 MATAAATGVWSEE325 pKa = 4.92 DD326 pKa = 3.29 EE327 pKa = 5.4 DD328 pKa = 4.13 FVSPSFFTSQRR339 pKa = 11.84 KK340 pKa = 8.17 PKK342 pKa = 10.58 NKK344 pKa = 10.13
Molecular weight: 39.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.643
IPC2_protein 6.825
IPC_protein 6.898
Toseland 6.751
ProMoST 7.527
Dawson 7.512
Bjellqvist 7.571
Wikipedia 7.454
Rodwell 7.512
Grimsley 6.825
Solomon 7.585
Lehninger 7.6
Nozaki 7.878
DTASelect 7.688
Thurlkill 7.732
EMBOSS 7.79
Sillero 7.966
Patrickios 4.317
IPC_peptide 7.571
IPC2_peptide 7.088
IPC2.peptide.svr19 7.142
Protein with the highest isoelectric point:
>tr|A0A346BPA9|A0A346BPA9_9VIRU Putative capsid protein OS=Cybaeus spider associated circular virus 1 OX=2293277 PE=4 SV=1
MM1 pKa = 7.1 VFRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 WPRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 VFGRR17 pKa = 11.84 KK18 pKa = 7.48 MRR20 pKa = 11.84 TSYY23 pKa = 10.55 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 SLRR29 pKa = 11.84 RR30 pKa = 11.84 KK31 pKa = 9.86 GNRR34 pKa = 11.84 KK35 pKa = 9.06 INKK38 pKa = 8.5 CAQLNTVFFKK48 pKa = 10.81 DD49 pKa = 3.49 VQLFTMSLDD58 pKa = 3.81 KK59 pKa = 11.16 DD60 pKa = 4.16 DD61 pKa = 4.29 NTPGEE66 pKa = 4.12 LNAAHH71 pKa = 7.09 FLHH74 pKa = 6.97 EE75 pKa = 4.28 IKK77 pKa = 10.28 PRR79 pKa = 11.84 EE80 pKa = 3.6 FGTRR84 pKa = 11.84 YY85 pKa = 10.72 KK86 pKa = 11.11 NMCAKK91 pKa = 9.86 HH92 pKa = 6.39 RR93 pKa = 11.84 YY94 pKa = 8.95 VKK96 pKa = 10.01 FLKK99 pKa = 8.96 WRR101 pKa = 11.84 YY102 pKa = 8.77 FIKK105 pKa = 10.52 LFQVTYY111 pKa = 9.18 DD112 pKa = 3.5 TVLLKK117 pKa = 10.28 KK118 pKa = 10.51 DD119 pKa = 3.54 PKK121 pKa = 10.89 DD122 pKa = 3.41 PNNAFLATPGINSISNLPFRR142 pKa = 11.84 INWDD146 pKa = 3.48 LNHH149 pKa = 7.19 IYY151 pKa = 10.06 TKK153 pKa = 10.83 GVAISDD159 pKa = 3.75 QFDD162 pKa = 3.72 DD163 pKa = 4.41 PNFKK167 pKa = 9.34 TVHH170 pKa = 6.26 AGMKK174 pKa = 9.85 KK175 pKa = 9.91 AVQFTFNVPKK185 pKa = 10.44 HH186 pKa = 5.06 LQHH189 pKa = 6.5 WVDD192 pKa = 3.85 GPTIGAIDD200 pKa = 3.75 VKK202 pKa = 10.87 QSIGRR207 pKa = 11.84 YY208 pKa = 9.19 LEE210 pKa = 3.72 QATNSKK216 pKa = 10.27 NFAAPRR222 pKa = 11.84 YY223 pKa = 8.7 FVGTASDD230 pKa = 3.48 LMKK233 pKa = 10.6 NQNEE237 pKa = 4.39 IIYY240 pKa = 9.69 SDD242 pKa = 3.47 RR243 pKa = 11.84 KK244 pKa = 10.07 EE245 pKa = 3.93 PPLRR249 pKa = 11.84 YY250 pKa = 9.04 FLYY253 pKa = 8.55 IQSYY257 pKa = 10.05 AYY259 pKa = 9.09 CTFKK263 pKa = 11.13 GLTEE267 pKa = 4.24 TDD269 pKa = 3.26 TT270 pKa = 3.89
Molecular weight: 31.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.253
IPC2_protein 9.633
IPC_protein 9.867
Toseland 10.365
ProMoST 9.999
Dawson 10.526
Bjellqvist 10.175
Wikipedia 10.687
Rodwell 10.979
Grimsley 10.584
Solomon 10.555
Lehninger 10.526
Nozaki 10.35
DTASelect 10.175
Thurlkill 10.394
EMBOSS 10.76
Sillero 10.438
Patrickios 10.643
IPC_peptide 10.555
IPC2_peptide 8.873
IPC2.peptide.svr19 8.652
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
614
270
344
307.0
35.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.515 ± 0.607
1.629 ± 0.328
5.537 ± 0.246
5.212 ± 1.659
7.003 ± 0.256
5.049 ± 0.383
2.28 ± 0.198
5.7 ± 0.326
8.469 ± 0.5
6.352 ± 0.434
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.466 ± 0.479
6.352 ± 0.199
4.723 ± 0.058
3.746 ± 0.027
6.84 ± 1.532
5.537 ± 0.927
6.026 ± 0.406
5.375 ± 0.12
1.954 ± 0.3
4.235 ± 0.368
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here